- EMDB-0630: The axonemal doublet microtubule focusing on the inner junction r... -
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基本情報
登録情報
データベース: EMDB / ID: EMD-0630
タイトル
The axonemal doublet microtubule focusing on the inner junction region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas pacrg; fap20 double mutant cilia
マップデータ
The axonemal doublet microtubule focusing on the inner junction region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas pacrg; fap20 double mutant cilia
試料
細胞器官・細胞要素: The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas pacrg; fap20 double mutant cilia
National Institutes of Health/National Institute of General Medical Sciences
R01GM112050
米国
National Institutes of Health/National Institute of General Medical Sciences
R01GM055667
米国
National Institutes of Health/National Institute of General Medical Sciences
R01GM083122
米国
引用
ジャーナル: Mol Biol Cell / 年: 2019 タイトル: PACRG and FAP20 form the inner junction of axonemal doublet microtubules and regulate ciliary motility. 著者: Erin E Dymek / Jianfeng Lin / Gang Fu / Mary E Porter / Daniela Nicastro / Elizabeth F Smith / 要旨: We previously demonstrated that PACRG plays a role in regulating dynein-driven microtubule sliding in motile cilia. To expand our understanding of the role of PACRG in ciliary assembly and motility, ...We previously demonstrated that PACRG plays a role in regulating dynein-driven microtubule sliding in motile cilia. To expand our understanding of the role of PACRG in ciliary assembly and motility, we used a combination of functional and structural studies, including newly identified mutants. Using cryo-electron tomography we show that PACRG and FAP20 form the inner junction between the A- and B-tubule along the length of all nine ciliary doublet microtubules. The lack of PACRG and FAP20 also results in reduced assembly of inner-arm dynein IDA and the beak-MIP structures. In addition, our functional studies reveal that loss of PACRG and/or FAP20 causes severe cell motility defects and reduced in vitro microtubule sliding velocities. Interestingly, the addition of exogenous PACRG and/or FAP20 protein to isolated mutant axonemes restores microtubule sliding velocities, but not ciliary beating. Taken together, these studies show that PACRG and FAP20 comprise the inner junction bridge that serves as a hub for both directly modulating dynein-driven microtubule sliding, as well as for the assembly of additional ciliary components that play essential roles in generating coordinated ciliary beating.
ダウンロード / ファイル: emd_0630.map.gz / 形式: CCP4 / 大きさ: 2 MB / タイプ: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
注釈
The axonemal doublet microtubule focusing on the inner junction region extracted from the cryo-electron tomography and subtomographic average of isolated Chlamydomonas pacrg; fap20 double mutant cilia
ボクセルのサイズ
X=Y=Z: 5.523 Å
密度
表面レベル
登録者による: 118 / ムービー #1: 118
最小 - 最大
109.69882 - 125.704895
平均 (標準偏差)
116.70518 (±3.4906275)
対称性
空間群: 1
詳細
EMDB XML:
マップ形状
Axis order
X
Y
Z
Origin
-73
-14
-21
サイズ
70
80
95
Spacing
80
70
95
セル
A: 441.83997 Å / B: 386.61 Å / C: 524.685 Å α=β=γ: 90.0 °
CCP4マップ ヘッダ情報:
mode
Image stored as Reals
Å/pix. X/Y/Z
5.523
5.523
5.523
M x/y/z
80
70
95
origin x/y/z
0.000
0.000
0.000
length x/y/z
441.840
386.610
524.685
α/β/γ
90.000
90.000
90.000
MAP C/R/S
1
2
3
start NC/NR/NS
-14
-73
-21
NC/NR/NS
80
70
95
D min/max/mean
109.699
125.705
116.705
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添付データ
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試料の構成要素
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全体 : The inner junction region of axonemal doublet microtubule average...
全体
名称: The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas pacrg; fap20 double mutant cilia
要素
細胞器官・細胞要素: The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas pacrg; fap20 double mutant cilia
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超分子 #1: The inner junction region of axonemal doublet microtubule average...
超分子
名称: The inner junction region of axonemal doublet microtubule averaged from Chlamydomonas pacrg; fap20 double mutant cilia タイプ: organelle_or_cellular_component / ID: 1 / 親要素: 0