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- EMDB-0197: Multi-body refined map of the rabbit 80S collided with a stalled 80S -
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Open data
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Basic information
Entry | Database: EMDB / ID: EMD-0197 | |||||||||||||||
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Title | Multi-body refined map of the rabbit 80S collided with a stalled 80S | |||||||||||||||
![]() | Multi-body refined rabbit 80S ribosome collided with the stalled 80S ribosome | |||||||||||||||
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![]() | Translation / Quality Control / Ribosome | |||||||||||||||
Function / homology | ![]() ribosomal subunit / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / phagocytic cup / 90S preribosome / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding ...ribosomal subunit / positive regulation of signal transduction by p53 class mediator / ubiquitin ligase inhibitor activity / phagocytic cup / 90S preribosome / ribosomal small subunit export from nucleus / rough endoplasmic reticulum / translation regulator activity / gastrulation / MDM2/MDM4 family protein binding / cytosolic ribosome / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / ribosomal large subunit biogenesis / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / positive regulation of apoptotic signaling pathway / maturation of SSU-rRNA / small-subunit processome / spindle / mRNA 5'-UTR binding / antimicrobial humoral immune response mediated by antimicrobial peptide / rRNA processing / rhythmic process / positive regulation of canonical Wnt signaling pathway / regulation of translation / large ribosomal subunit / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / 5S rRNA binding / ribosomal large subunit assembly / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / small ribosomal subunit rRNA binding / defense response to Gram-negative bacterium / killing of cells of another organism / perikaryon / cytosolic large ribosomal subunit / cytoplasmic translation / cell differentiation / tRNA binding / mitochondrial inner membrane / postsynaptic density / rRNA binding / ribosome / structural constituent of ribosome / translation / ribonucleoprotein complex / cell division / DNA repair / mRNA binding / apoptotic process / synapse / centrosome / dendrite / negative regulation of apoptotic process / nucleolus / perinuclear region of cytoplasm / endoplasmic reticulum / Golgi apparatus / DNA binding / RNA binding / zinc ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | single particle reconstruction / cryo EM / Resolution: 6.5 Å | |||||||||||||||
![]() | Juszkiewicz S / Chandrasekaran V | |||||||||||||||
Funding support | ![]() ![]()
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![]() | ![]() Title: ZNF598 Is a Quality Control Sensor of Collided Ribosomes. Authors: Szymon Juszkiewicz / Viswanathan Chandrasekaran / Zhewang Lin / Sebastian Kraatz / V Ramakrishnan / Ramanujan S Hegde / ![]() Abstract: Aberrantly slow translation elicits quality control pathways initiated by the ubiquitin ligase ZNF598. How ZNF598 discriminates physiologic from pathologic translation complexes and ubiquitinates ...Aberrantly slow translation elicits quality control pathways initiated by the ubiquitin ligase ZNF598. How ZNF598 discriminates physiologic from pathologic translation complexes and ubiquitinates stalled ribosomes selectively is unclear. Here, we find that the minimal unit engaged by ZNF598 is the collided di-ribosome, a molecular species that arises when a trailing ribosome encounters a slower leading ribosome. The collided di-ribosome structure reveals an extensive 40S-40S interface in which the ubiquitination targets of ZNF598 reside. The paucity of 60S interactions allows for different ribosome rotation states, explaining why ZNF598 recognition is indifferent to how the leading ribosome has stalled. The use of ribosome collisions as a proxy for stalling allows the degree of tolerable slowdown to be tuned by the initiation rate on that mRNA; hence, the threshold for triggering quality control is substrate specific. These findings illustrate how higher-order ribosome architecture can be exploited by cellular factors to monitor translation status. | |||||||||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 23.7 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 100 KB 100 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 17.6 KB | Display | ![]() |
Images | ![]() | 157.7 KB | ||
Masks | ![]() | 476.8 MB | ![]() | |
Filedesc metadata | ![]() | 19.6 KB | ||
Others | ![]() ![]() | 325.9 MB 324.6 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 755.4 KB | Display | ![]() |
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Full document | ![]() | 755 KB | Display | |
Data in XML | ![]() | 25.2 KB | Display | |
Data in CIF | ![]() | 34.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6hcqMC ![]() 0192C ![]() 0194C ![]() 0195C ![]() 6hcfC ![]() 6hcjC ![]() 6hcmC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | Multi-body refined rabbit 80S ribosome collided with the stalled 80S ribosome | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 2.14 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
-Mask #1
File | ![]() | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: Body2 half1 map
File | emd_0197_half_map_1.map | ||||||||||||
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Annotation | Body2 half1 map | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Body2 half1 map
File | emd_0197_half_map_2.map | ||||||||||||
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Annotation | Body2 half1 map | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
+Entire : Rabbit 80S on globin mRNA in the rotated state with A/P and P/E tRNAs
+Supramolecule #1: Rabbit 80S on globin mRNA in the rotated state with A/P and P/E tRNAs
+Macromolecule #1: 28S ribosomal RNA
+Macromolecule #2: 5S ribosomal RNA
+Macromolecule #3: 5.8S ribosomal RNA
+Macromolecule #4: 18S ribosomal RNA
+Macromolecule #79: A/P tRNA
+Macromolecule #82: mRNA
+Macromolecule #83: P/E tRNA
+Macromolecule #5: uS2
+Macromolecule #6: 40S ribosomal protein S3a
+Macromolecule #7: eS1
+Macromolecule #8: uS3
+Macromolecule #9: eS4
+Macromolecule #10: Ribosomal protein S5
+Macromolecule #11: 40S ribosomal protein S6
+Macromolecule #12: 40S ribosomal protein S7
+Macromolecule #13: eS8
+Macromolecule #14: Ribosomal protein S9 (Predicted)
+Macromolecule #15: eS10
+Macromolecule #16: Ribosomal protein S11
+Macromolecule #17: 40S ribosomal protein S12
+Macromolecule #18: uS15
+Macromolecule #19: uS11
+Macromolecule #20: uS19
+Macromolecule #21: Ribosomal protein S16
+Macromolecule #22: eS17
+Macromolecule #23: uS13
+Macromolecule #24: eS19
+Macromolecule #25: uS10
+Macromolecule #26: eS21
+Macromolecule #27: Ribosomal protein S15a
+Macromolecule #28: uS12
+Macromolecule #29: eS24
+Macromolecule #30: eS25
+Macromolecule #31: eS26
+Macromolecule #32: 40S ribosomal protein S27
+Macromolecule #33: Ribosomal protein S28
+Macromolecule #34: uS14
+Macromolecule #35: 40S ribosomal protein S30
+Macromolecule #36: Ribosomal protein S27a
+Macromolecule #37: RACK1
+Macromolecule #38: Ribosomal protein L8
+Macromolecule #39: uL3
+Macromolecule #40: uL4
+Macromolecule #41: 60S ribosomal protein L5
+Macromolecule #42: 60S ribosomal protein L6
+Macromolecule #43: uL30
+Macromolecule #44: eL8
+Macromolecule #45: uL6
+Macromolecule #46: 60S ribosomal protein L10
+Macromolecule #47: Ribosomal protein L11
+Macromolecule #48: eL13
+Macromolecule #49: Ribosomal protein L14
+Macromolecule #50: Ribosomal protein L15
+Macromolecule #51: uL13
+Macromolecule #52: uL22
+Macromolecule #53: eL18
+Macromolecule #54: eL19
+Macromolecule #55: eL20
+Macromolecule #56: eL21
+Macromolecule #57: eL22
+Macromolecule #58: Ribosomal protein L23
+Macromolecule #59: uL23
+Macromolecule #60: Ribosomal protein L26
+Macromolecule #61: 60S ribosomal protein L27
+Macromolecule #62: uL15
+Macromolecule #63: eL29
+Macromolecule #64: eL30
+Macromolecule #65: eL31
+Macromolecule #66: eL32
+Macromolecule #67: eL33
+Macromolecule #68: eL34
+Macromolecule #69: uL29
+Macromolecule #70: 60S ribosomal protein L36
+Macromolecule #71: Ribosomal protein L37
+Macromolecule #72: eL38
+Macromolecule #73: eL39
+Macromolecule #74: eL40
+Macromolecule #75: eL41
+Macromolecule #76: eL42
+Macromolecule #77: eL43
+Macromolecule #78: eL28
+Macromolecule #80: uL10
+Macromolecule #81: Ribosomal protein L12
+Macromolecule #84: Ribosomal protein
+Macromolecule #85: nascent chain
+Macromolecule #86: MAGNESIUM ION
+Macromolecule #87: ZINC ION
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON III (4k x 4k) / Detector mode: INTEGRATING / Average electron dose: 1.79 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |