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- SASDGV6: Cysteine synthase A (CysK) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDGV6
SampleCysteine synthase A
  • Cysteine synthase A (protein), CysK, Escherichia coli K-12
Function / homology
Function and homology information


S-carboxymethylcysteine synthase / S-carboxymethylcysteine synthase activity / cysteine synthase complex / cystathionine beta-synthase activity / cysteine synthase / L-cysteine desulfhydrase activity / cysteine synthase activity / cysteine biosynthetic process from serine / amino acid biosynthetic process / pyridoxal phosphate binding ...S-carboxymethylcysteine synthase / S-carboxymethylcysteine synthase activity / cysteine synthase complex / cystathionine beta-synthase activity / cysteine synthase / L-cysteine desulfhydrase activity / cysteine synthase activity / cysteine biosynthetic process from serine / amino acid biosynthetic process / pyridoxal phosphate binding / transferase activity / protein homodimerization activity / identical protein binding / cytosol / cytoplasm
Similarity search - Function
Cysteine synthase CysK / Cysteine synthase / Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site / Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. / Pyridoxal-phosphate dependent enzyme / Tryptophan synthase beta subunit-like PLP-dependent enzyme / Pyridoxal-phosphate dependent enzyme
Similarity search - Domain/homology
Biological speciesEscherichia coli K-12 (bacteria)
CitationDate: 2019 Oct 21
Title: Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action
Authors: Rosa B / Marchetti M / Paredi G / Amenitsch H / Franko N / Benoni R / Giabbai B / De Marino M / Mozzarelli A / Ronda L / Storici P / Campanini B
Contact author
  • Heinz Amenitsch (Graz University of Technology)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #3856
Type: dummy / Radius of dummy atoms: 2.25 A / Symmetry: P1 / Chi-square value: 1.873 / P-value: 0.047316
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Cysteine synthase A / Specimen concentration: 1.2 mg/ml
BufferName: 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE
pH: 7.5
Entity #1926Name: CysK / Type: protein / Description: Cysteine synthase A / Formula weight: 35.712 / Num. of mol.: 2 / Source: Escherichia coli K-12 / References: UniProt: P0ABK5
Sequence: MGKIFEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASNPEKYLL LQQFSNPANP EIHEKTTGPE IWEDTDGQVD ...Sequence:
MGKIFEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASNPEKYLL LQQFSNPANP EIHEKTTGPE IWEDTDGQVD VFIAGVGTGG TLTGVSRYIK GTKGKTDLIS VAVEPTDSPV IAQALAGEEI KPGPHKIQGI GAGFIPANLD LKLVDKVIGI TNEEAISTAR RLMEEEGILA GISSGAAVAA ALKLQEDESF TNKNIVVILP SSGERYLSTA LFADLFTEKE LQQTSLEHHH HHH

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Experimental information

BeamInstrument name: ELETTRA - Sincrotrone Trieste Austrian SAXS beamline 5.2L
City: Trieste / 国: Italy / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 1.2324 mm
DetectorName: Dectris / Pilatus3 1M / Pixsize x: 0.172 mm
Scan
Title: Cysteine synthase A / Measurement date: Jun 1, 2016 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 10 sec. / Number of frames: 12 / Unit: 1/nm /
MinMax
Q0.1554 4.0761
Distance distribution function P(R)
Sofotware P(R): GNOM 4.6 / Number of points: 308 /
MinMax
Q0.2712 3.737
P(R) point21 328
R0 8.5
Result
Type of curve: single_conc /
ExperimentalPorod
MW72 kDa72.3 kDa
Volume-108.42 nm3

P(R)GuinierGuinier error
Forward scattering, I06.7 6.71 0.013
Radius of gyration, Rg2.62 nm2.64 nm0.054

MinMax
D-8.5
Guinier point8 60

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