SASDGV6: Cysteine synthase A (CysK)

Basic information

• Entry: Database: SASBDB / ID: SASDGV6

Sample

Cysteine synthase A

• Cysteine synthase A (protein), CysK, Escherichia coli K-12

Function / homology

Function:

S-carboxymethylcysteine synthase / S-carboxymethylcysteine synthase activity / cysteine synthase complex / L-cysteine desulfhydrase activity / cysteine synthase / cysteine synthase activity / cysteine biosynthetic process from serine / amino acid biosynthetic process / pyridoxal phosphate binding / transferase activity / protein homodimerization activity / identical protein binding / cytoplasm / cytosol

Domain/homology:

Cysteine synthase CysK / Cysteine synthase / Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site / Cysteine synthase/cystathionine beta-synthase P-phosphate attachment site. / : / Pyridoxal-phosphate dependent enzyme / Pyridoxal-phosphate dependent enzyme / Tryptophan synthase beta subunit-like PLP-dependent enzyme

Component:

Cysteine synthase A

• Biological species: Escherichia coli K-12 (bacteria)
• Citation: Date: 2019 Oct 21; Title: Combination of SAXS and Protein Painting Discloses the Three-Dimensional Organization of the Bacterial Cysteine Synthase Complex, a Potential Target for Enhancers of Antibiotic Action; Authors: Rosa B / Marchetti M / Paredi G / Amenitsch H / Franko N / Benoni R / Giabbai B / De Marino M / Mozzarelli A / Ronda L / Storici P / Campanini B

Contact author

• Heinz Amenitsch (Graz University of Technology)

Models

• Model #3856: ; Type: dummy / Radius of dummy atoms: 2.25 A / Symmetry: P1 / Chi-square value: 1.873 / P-value: 0.047316; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Cysteine synthase A / Specimen concentration: 1.2 mg/ml
• Buffer: Name: 20 mM sodium phosphate, 85 mM NaCl, 2 mM EDTA, 10 mM 2-MCE; pH: 7.5

Entity #1926

Name: CysK / Type: protein / Description: Cysteine synthase A / Formula weight: 35.712 / Num. of mol.: 2 / Source: Escherichia coli K-12 / References: UniProt: P0ABK5
Sequence:

MGKIFEDNSL TIGHTPLVRL NRIGNGRILA KVESRNPSFS VKCRIGANMI WDAEKRGVLK PGVELVEPTS GNTGIALAYV AAARGYKLTL TMPETMSIER RKLLKALGAN LVLTEGAKGM KGAIQKAEEI VASNPEKYLL LQQFSNPANP EIHEKTTGPE IWEDTDGQVD VFIAGVGTGG TLTGVSRYIK GTKGKTDLIS VAVEPTDSPV IAQALAGEEI KPGPHKIQGI GAGFIPANLD LKLVDKVIGI TNEEAISTAR RLMEEEGILA GISSGAAVAA ALKLQEDESF TNKNIVVILP SSGERYLSTA LFADLFTEKE LQQTSLEHHH HHH

Experimental information

• Beam: Instrument name: ELETTRA - Sincrotrone Trieste Austrian SAXS beamline 5.2L; City: Trieste / 国: Italy / Type of source: X-ray synchrotron / Wavelength: 0.15 Å / Dist. spec. to detc.: 1.2324 mm
• Detector: Name: Dectris / Pilatus3 1M / Pixsize x: 0.172 mm

Scan

Title: Cysteine synthase A / Measurement date: Jun 1, 2016 / Storage temperature: 4 °C / Cell temperature: 20 °C / Exposure time: 10 sec. / Number of frames: 12 / Unit: 1/nm /

	Min	Max
Q	0.1554	4.0761

Distance distribution function P(R)

Sofotware P(R): GNOM 4.6 / Number of points: 308 /

	Min	Max
Q	0.2712	3.737
P(R) point	21	328
R	0	8.5

Result

Type of curve: single_conc /

	Experimental	Porod
MW	72 kDa	72.3 kDa
Volume	-	108.42 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	6.7	6.71	0.013
Radius of gyration, Rg	2.62 nm	2.64 nm	0.054

	Min	Max
D	-	8.5
Guinier point	8	60