SASDF76: Polyphosphate-targeting protein A (PptA)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDF76

試料

Polyphosphate-targeting protein A

• Polyphosphate-targeting protein A (protein), PptA, Streptomyces chartreusis

• 機能・相同性: CHAD domain superfamily / CHAD domain / CHAD / CHAD domain / CHAD domain profile. / CHAD domain protein
• 生物種: Streptomyces chartreusis (バクテリア)
• 引用: ジャーナル: FEBS Lett / 年: 2019; タイトル: Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold.; 著者: Sebastiaan Werten / Nils Hinnerk Rustmeier / Maximilian Gemmer / Marie-Joëlle Virolle / Winfried Hinrichs / ; 要旨: X-ray crystallographic analysis of a phosin (PptA) from Steptomyces chartreusis reveals a metal-associated, lozenge-shaped fold featuring a 5-10 Å wide, positively charged tunnel that traverses the protein core. Two distinct metal-binding sites were identified in which the predominant metal ion was Cu . In solution, PptA forms stable homodimers that bind with nanomolar affinity to polyphosphate, a stress-related biopolymer acting as a phosphate and energy reserve in conditions of nutrient depletion. A single protein dimer interacts with 14-15 consecutive phosphate moieties within the polymer. Our observations suggest that PptA plays a role in polyphosphate metabolism, mobilisation or sensing, possibly by acting in concert with polyphosphate kinase (Ppk). Like Ppk, phosins may influence antibiotic synthesis by streptomycetes.

登録者

• Sebastiaan Werten (Medical University of Innsbruck, Innsbruck, Austria)

モデル

• モデル #2974: ; タイプ: mix / ダミー原子の半径: 1.90 A / 対称性: p2 / カイ2乗値: 1.06 / P-value: 0.555714; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Polyphosphate-targeting protein A / 試料濃度: 1.31-5.48
• バッファ: 名称: 20 mM Tris-HCl 400 mM NaCl / pH: 7.4

要素 #1631

名称: PptA / タイプ: protein / 記述: Polyphosphate-targeting protein A / 分子量: 39.59 / 分子数: 2 / 由来: Streptomyces chartreusis / 参照: UniProt: A0A2N9BBV4
配列:

GHMAQRHLDP TDPLAGPSPT GDTLAGYLRA QATEFLRALR LHRETGSGAN GAEGPVEAAR ALRRSARRIS ATLHTFQSLL DTDWCEGMRP ELAWVSGTLA MEHAYTARLE RLLNALHRLS GSTALPSQTA DSAAGGRAAG AAPVPRTAAP RDAGLRTPPT STTERGNLTV GAAKAGALLD RQLTLARTRA HSTALQAMGS SRFHAIADKV AVLASEVPLT PAAATADLRP LATAAKDRLT DAVAALPLIT AGHPYNAAAL IHGLSPDTVP HPQDAPWHQV RLLLRLHRYA REAVSGPKGN AVVDLRLLSA GQALNRHRDA SEAAAAAAQA ARTPRIAPAT AYALGVLHAD QRHEVEAARF AFQQAWQKEA EAVSTR

実験情報

• ビーム: 設備名称: PETRA III EMBL P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.124 Å / スペクトロメータ・検出器間距離: 3.1 mm
• 検出器: 名称: Pilatus 2M

スキャン

タイトル: Polyphosphate-targeting protein A / 測定日: 2016年11月24日 / セル温度: 10 °C / 照射時間: 0.05 sec. / フレーム数: 20 / 単位: 1/nm /

	Min	Max
Q	0.0334	3.4982

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 1056 /

	Min	Max
Q	0.0792223	2.27864
P(R) point	1	1056
R	0	11.84

結果

カーブのタイプ: merged

	実験値	Standard	Porod
分子量	78.5 kDa	58.8 kDa	77.3 kDa
体積	-	-	123.74 nm3

	P(R)	Guinier	Guinier error
前方散乱 I0	4523	4530.06	11.91
慣性半径, Rg	3.536 nm	3.51 nm	0.28

	Min	Max
D	-	11.84
Guinier point	23	162