- SASDF76: Polyphosphate-targeting protein A (PptA) -
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Open data
ID or keywords:
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Basic information
Entry
Database: SASBDB / ID: SASDF76
Sample
Polyphosphate-targeting protein A
Polyphosphate-targeting protein A (protein), PptA, Streptomyces chartreusis
Function / homology
CHAD domain superfamily / CHAD domain / CHAD / CHAD domain / CHAD domain profile. / CHAD domain protein
Function and homology information
Biological species
Streptomyces chartreusis (bacteria)
Citation
Journal: FEBS Lett / Year: 2019 Title: Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold. Authors: Sebastiaan Werten / Nils Hinnerk Rustmeier / Maximilian Gemmer / Marie-Joëlle Virolle / Winfried Hinrichs / Abstract: X-ray crystallographic analysis of a phosin (PptA) from Steptomyces chartreusis reveals a metal-associated, lozenge-shaped fold featuring a 5-10 Å wide, positively charged tunnel that traverses the ...X-ray crystallographic analysis of a phosin (PptA) from Steptomyces chartreusis reveals a metal-associated, lozenge-shaped fold featuring a 5-10 Å wide, positively charged tunnel that traverses the protein core. Two distinct metal-binding sites were identified in which the predominant metal ion was Cu . In solution, PptA forms stable homodimers that bind with nanomolar affinity to polyphosphate, a stress-related biopolymer acting as a phosphate and energy reserve in conditions of nutrient depletion. A single protein dimer interacts with 14-15 consecutive phosphate moieties within the polymer. Our observations suggest that PptA plays a role in polyphosphate metabolism, mobilisation or sensing, possibly by acting in concert with polyphosphate kinase (Ppk). Like Ppk, phosins may influence antibiotic synthesis by streptomycetes.
Contact author
Sebastiaan Werten (Medical University of Innsbruck, Innsbruck, Austria)
Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
Detector
Name: Pilatus 2M
Scan
Title: Polyphosphate-targeting protein A / Measurement date: Nov 24, 2016 / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
Min
Max
Q
0.0334
3.4982
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1056 /
Min
Max
Q
0.0792223
2.27864
P(R) point
1
1056
R
0
11.84
Result
Type of curve: merged
Experimental
Standard
Porod
MW
78.5 kDa
58.8 kDa
77.3 kDa
Volume
-
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123.74 nm3
P(R)
Guinier
Guinier error
Forward scattering, I0
4523
4530.06
11.91
Radius of gyration, Rg
3.536 nm
3.51 nm
0.28
Min
Max
D
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11.84
Guinier point
23
162
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