SASDF76: Polyphosphate-targeting protein A (PptA)

Basic information

• Entry: Database: SASBDB / ID: SASDF76

Sample

Polyphosphate-targeting protein A

• Polyphosphate-targeting protein A (protein), PptA, Streptomyces chartreusis

• Function / homology: CHAD domain superfamily / CHAD domain / CHAD / CHAD domain / CHAD domain profile. / CHAD domain protein
• Biological species: Streptomyces chartreusis (bacteria)
• Citation: Journal: FEBS Lett / Year: 2019; Title: Structural and biochemical analysis of a phosin from Streptomyces chartreusis reveals a combined polyphosphate- and metal-binding fold.; Authors: Sebastiaan Werten / Nils Hinnerk Rustmeier / Maximilian Gemmer / Marie-Joëlle Virolle / Winfried Hinrichs / ; Abstract: X-ray crystallographic analysis of a phosin (PptA) from Steptomyces chartreusis reveals a metal-associated, lozenge-shaped fold featuring a 5-10 Å wide, positively charged tunnel that traverses the protein core. Two distinct metal-binding sites were identified in which the predominant metal ion was Cu . In solution, PptA forms stable homodimers that bind with nanomolar affinity to polyphosphate, a stress-related biopolymer acting as a phosphate and energy reserve in conditions of nutrient depletion. A single protein dimer interacts with 14-15 consecutive phosphate moieties within the polymer. Our observations suggest that PptA plays a role in polyphosphate metabolism, mobilisation or sensing, possibly by acting in concert with polyphosphate kinase (Ppk). Like Ppk, phosins may influence antibiotic synthesis by streptomycetes.

Contact author

• Sebastiaan Werten (Medical University of Innsbruck, Innsbruck, Austria)

Models

• Model #2974: ; Type: mix / Radius of dummy atoms: 1.90 A / Symmetry: p2 / Chi-square value: 1.06 / P-value: 0.555714; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Polyphosphate-targeting protein A / Specimen concentration: 1.31-5.48
• Buffer: Name: 20 mM Tris-HCl 400 mM NaCl / pH: 7.4

Entity #1631

Name: PptA / Type: protein / Description: Polyphosphate-targeting protein A / Formula weight: 39.59 / Num. of mol.: 2 / Source: Streptomyces chartreusis / References: UniProt: A0A2N9BBV4
Sequence:

GHMAQRHLDP TDPLAGPSPT GDTLAGYLRA QATEFLRALR LHRETGSGAN GAEGPVEAAR ALRRSARRIS ATLHTFQSLL DTDWCEGMRP ELAWVSGTLA MEHAYTARLE RLLNALHRLS GSTALPSQTA DSAAGGRAAG AAPVPRTAAP RDAGLRTPPT STTERGNLTV GAAKAGALLD RQLTLARTRA HSTALQAMGS SRFHAIADKV AVLASEVPLT PAAATADLRP LATAAKDRLT DAVAALPLIT AGHPYNAAAL IHGLSPDTVP HPQDAPWHQV RLLLRLHRYA REAVSGPKGN AVVDLRLLSA GQALNRHRDA SEAAAAAAQA ARTPRIAPAT AYALGVLHAD QRHEVEAARF AFQQAWQKEA EAVSTR

Experimental information

• Beam: Instrument name: PETRA III EMBL P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3.1 mm
• Detector: Name: Pilatus 2M

Scan

Title: Polyphosphate-targeting protein A / Measurement date: Nov 24, 2016 / Cell temperature: 10 °C / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /

	Min	Max
Q	0.0334	3.4982

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 1056 /

	Min	Max
Q	0.0792223	2.27864
P(R) point	1	1056
R	0	11.84

Result

Type of curve: merged

	Experimental	Standard	Porod
MW	78.5 kDa	58.8 kDa	77.3 kDa
Volume	-	-	123.74 nm3

	P(R)	Guinier	Guinier error
Forward scattering, I0	4523	4530.06	11.91
Radius of gyration, Rg	3.536 nm	3.51 nm	0.28

	Min	Max
D	-	11.84
Guinier point	23	162