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- SASDEV7: Stator protein complex FlaG/FlaF-I96Y (Stator protein FlaG solubl... -

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Basic information

Entry
Database: SASBDB / ID: SASDEV7
SampleStator protein complex FlaG/FlaF-I96Y
  • Stator protein FlaG soluble domain (protein), sFlaG, Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
  • Conserved flagellar protein FlaF-I96Y soluble domain (protein), sFlaF-I96Y, Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
Function / homologyFlagellin, archaea / Archaebacterial flagellin / archaeal or bacterial-type flagellum-dependent cell motility / structural molecule activity / membrane / identical protein binding / Flagellar protein FlaG / Conserved flagellar protein F
Function and homology information
Biological speciesSulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770) (acidophilic)
Contact author
  • Chi-Lin Tsai (MD Anderson Cancer Center)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #2618
Type: mix / Comment: The His-tag is modeled by Modeller / Chi-square value: 10.0384318060461
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Stator protein complex FlaG/FlaF-I96Y / Specimen concentration: 1.50-3.00 / Entity id: 1381 / 1383
BufferName: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol
pH: 3
Entity #1381Name: sFlaG / Type: protein / Description: Stator protein FlaG soluble domain / Formula weight: 14.932 / Num. of mol.: 2
Source: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
References: UniProt: Q4J9K7
Sequence:
MGSSHHHHHH SQDPNSISTT MSSYSIQQSQ KMLTQLQIDY ATNTSSNTVV AYLHNVGETT ISYLQNSVVY FGPNGQLQPV GYNSGSSPYW TVTSNSLQPG SVVKIIIYLS SPLSSNQYYT IQIVTPNGYT VSYMF
Entity #1383Name: sFlaF-I96Y / Type: protein
Description: Conserved flagellar protein FlaF-I96Y soluble domain
Formula weight: 16.289 / Num. of mol.: 2
Source: Sulfolobus acidocaldarius (strain ATCC 33909 / DSM 639 / JCM 8929 / NBRC 15157 / NCIMB 11770)
References: UniProt: Q4J9K8
Sequence:
MGSSHHHHHH SQDPNSNQAQ ELNHELELEQ LETKITVSSV SLTGSTLNVV LENNGSTNLY DFQGFSVIVQ YYANISNYST FNLSLYNYTK NSNPSPYYWT INTPLLAPGS QATLTIILPY PPYPNTQATV VIVTNYGPSV IWRGSL

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Experimental information

BeamInstrument name: Advanced Light Source (ALS) 12.3.1 (SIBYLS)
City: Berkeley, CA / 国: USA / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.127 Å / Dist. spec. to detc.: 1.5 mm
DetectorName: Pilatus3 X 2M / Pixsize x: 172 mm
Scan
Title: Stator protein complex FlaG/FlaF-I96Y / Measurement date: Nov 10, 2016 / Cell temperature: 10 °C / Exposure time: 0.3 sec. / Number of frames: 33 / Unit: 1/A /
MinMax
Q0.0125 0.3698
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 476 /
MinMax
Q0.01338 0.5427
P(R) point1 476
R0 82
Result
Type of curve: merged /
ExperimentalPorod
MW50.85 kDa56 kDa
Volume-90 nm3

P(R)GuinierGuinier error
Forward scattering, I0208.5 208.48 0.22
Radius of gyration, Rg2.67 nm2.675 nm0.004

MinMax
D-8.2
Guinier point1 62

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