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- SASDE72: Ribonuclease E from Francisella tularensis (Endoribonuclease E, R... -

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Basic information

Entry
Database: SASBDB / ID: SASDE72
SampleRibonuclease E from Francisella tularensis
  • Endoribonuclease E (protein), RNase E, Francisella tularensis
Function / homology
Function and homology information


ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding / magnesium ion binding ...ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding / magnesium ion binding / zinc ion binding / cytoplasm
Similarity search - Function
Ribonuclease E/G / RNA-binding protein AU-1/Ribonuclease E/G / Ribonuclease E / Ribonuclease E/G family / RNA-binding domain, S1 / S1 domain profile. / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / S1 domain / Nucleic acid-binding, OB-fold
Similarity search - Domain/homology
Biological speciesFrancisella tularensis (bacteria)
CitationDate: 2019 Dec
Title: A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties
Authors: Mardle C / Shakespeare T / Butt L / Goddard L / Gowers D / Atkins H / Vincent H
Contact author
  • A Callaghan (University of Portsmouth)

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Structure visualization

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Models

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Sample

SampleName: Ribonuclease E from Francisella tularensis / Specimen concentration: 6.41 mg/ml
BufferName: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris / pH: 8
Entity #1205Name: RNase E / Type: protein / Description: Endoribonuclease E / Formula weight: 64.088 / Num. of mol.: 4 / Source: Francisella tularensis / References: UniProt: Q5NFK7
Sequence: HHHHHHHHHH SSGHIEGRHM KRILINSKSG EETRIATLDN GKLIDLDIES VDREQKKANI YKGYISRIEP SLNAIFVNYG EEKNGFLPFK EVSEYYLKDV PNGENIAPLL SEGQELIVQI DKEERGDKGA ALTTFITLAG SYMVLLPNNP EGGGISRRVE GEDRERLKKY ...Sequence:
HHHHHHHHHH SSGHIEGRHM KRILINSKSG EETRIATLDN GKLIDLDIES VDREQKKANI YKGYISRIEP SLNAIFVNYG EEKNGFLPFK EVSEYYLKDV PNGENIAPLL SEGQELIVQI DKEERGDKGA ALTTFITLAG SYMVLLPNNP EGGGISRRVE GEDRERLKKY LKELNIPKNM SVIARTACVE CSFEELKHDF DTLVELWGSI SQAYHRIKKP ALLHKESDII VRTVRDHLKE DVKEIIVDSK ECFEDVKRQL SLLRQSFDIN KVKLYNEELP LFAQFGIDQQ IENAYKREIR LPSGGSIVID TTEALVAIDV NSSRANKAED VETTAFKTNL EAAEEVARQL RIRDLGGLVI VDFIDMSFYP NRKQVEEKLM EALQQDRARI QMSRISKLGL VEISRQRLSS SINESVMQKC PRCEGHGFIK TTQATALTIL RKIRAEAIKE DTNEIRVQVP VDIAAYILNE KRDSIVEIER ISQVKVMIIP NFNMESPKFQ MQRIWGTSYK SNRTSSELIE DVYNIEIPKA GKKKIAAVDL NQAIENQAAS NTQEQKPQTD AQ

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.014 mm
DetectorName: Pilatus 2M
Scan
Title: Ribonuclease E from Francisella tularensis / Measurement date: Feb 11, 2017 / Storage temperature: 21 °C / Cell temperature: 15 °C / Exposure time: 3 sec. / Number of frames: 640 / Unit: 1/A /
MinMax
Q0.004 0.442
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 624 /
MinMax
Q0.00689906 0.157409
P(R) point1 624
R0 172.3
Result
Type of curve: single_conc /
ExperimentalPorod
MW290 kDa306 kDa
Volume-491 nm3

P(R)GuinierGuinier error
Forward scattering, I00.02788 0.02792 6.0E-5
Radius of gyration, Rg5.07 nm5.084 nm0.02

MinMax
D-17.23
Guinier point13 90

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