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- SASDE62: Ribonuclease E from Yersinia pestis (Endoribonuclease E, RNase E) -

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Open data


ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDE62
SampleRibonuclease E from Yersinia pestis
  • Endoribonuclease E (protein), RNase E, Yersinia pestis
Function / homology
Function and homology information


ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA nuclease activity / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding ...ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA nuclease activity / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding / magnesium ion binding / zinc ion binding / cytoplasm
Similarity search - Function
Polyribonucleotide phosphorylase C-terminal / Polyribonucleotide phosphorylase C terminal / : / RNase E/G, Thioredoxin-like domain / Ribonuclease E/G / Ribonuclease E / RNA-binding protein AU-1/Ribonuclease E/G / Ribonuclease E/G family / S1 domain profile. / Ribosomal protein S1-like RNA-binding domain ...Polyribonucleotide phosphorylase C-terminal / Polyribonucleotide phosphorylase C terminal / : / RNase E/G, Thioredoxin-like domain / Ribonuclease E/G / Ribonuclease E / RNA-binding protein AU-1/Ribonuclease E/G / Ribonuclease E/G family / S1 domain profile. / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / S1 domain / Nucleic acid-binding, OB-fold
Similarity search - Domain/homology
Biological speciesYersinia pestis (bacteria)
CitationDate: 2019 Dec
Title: A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties
Authors: Mardle C / Shakespeare T / Butt L / Goddard L / Gowers D / Atkins H / Vincent H
Contact author
  • A Callaghan (University of Portsmouth)

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Structure visualization

Downloads & links

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Models

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Sample

SampleName: Ribonuclease E from Yersinia pestis / Specimen concentration: 6.33 mg/ml
BufferName: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris / pH: 8
Entity #1204Name: RNase E / Type: protein / Description: Endoribonuclease E / Formula weight: 61.892 / Num. of mol.: 4 / Source: Yersinia pestis / References: UniProt: Q74TC3
Sequence: HHHHHHHHHH SGSSGHIEGR HMKRMLINAT QQEELRVALV DGQRLYDLDI ESPGHEQKKA NIYKGKITRI EPSLEAAFVD YGAERHGFLP LKEISREYFP NNYSSHGRPN IKDVLREGQE VIVQVDKEER GNKGAALTTF ISLAGSYLVL MPNNPRAGGI SRRIEGDDRT ...Sequence:
HHHHHHHHHH SGSSGHIEGR HMKRMLINAT QQEELRVALV DGQRLYDLDI ESPGHEQKKA NIYKGKITRI EPSLEAAFVD YGAERHGFLP LKEISREYFP NNYSSHGRPN IKDVLREGQE VIVQVDKEER GNKGAALTTF ISLAGSYLVL MPNNPRAGGI SRRIEGDDRT ELKEALSSLQ LPDGMGLIVR TAGVGKSADA LQWDLSFRLK HWDAIKKAAE GRPAPFLIHQ ESNVIVRAFR DYLRPDIGEI LIDNPKVLEL AKEHIAALGR PDFSSKIKLY SGEIPLFSHY QIESQIESAF QREVRLPSGG SIVIDTTEAL TAIDINSARA TRGGDIEETA FNTNLEAADE IARQLRLRDL GGLIVIDFID MTPVRHQREV ENRLRDAVRQ DRARIQIGRI SRFGLLEMSR QRLSPSLGES SHHVCPRCSG TGTVRDNESL SLSILRLIEE EALKENTHEV HAIVPVQIAS YLLNEKRESV NAIEKRQGGV RAVIVPNDQM QTPHYSVLRV RKGEEVPSLS YLLPQLHEAE MAQPLEEATI ERKRPEQPAL

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.014 mm
DetectorName: Pilatus 2M
Scan
Title: Ribonuclease E from Yersinia pestis / Measurement date: Feb 11, 2017 / Storage temperature: 21 °C / Cell temperature: 15 °C / Exposure time: 3 sec. / Number of frames: 640 / Unit: 1/A /
MinMax
Q0.004 0.442
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 621 /
MinMax
Q0.00834859 0.158134
P(R) point1 621
R0 164.4
Result
Type of curve: single_conc /
ExperimentalPorod
MW277 kDa294 kDa
Volume-470 nm3

P(R)GuinierGuinier error
Forward scattering, I00.02336 0.02333 5.7E-5
Radius of gyration, Rg5.07 nm5.059 nm0.01

MinMax
D-16.44
Guinier point19 90

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