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- SASDE82: Ribonuclease E from Burkholderia pseudomallei (Endoribonuclease E... -

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Basic information

Entry
Database: SASBDB / ID: SASDE82
SampleRibonuclease E from Burkholderia pseudomallei
  • Endoribonuclease E (protein), RNase E, Burkholderia pseudomallei
Function / homology
Function and homology information


ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding / magnesium ion binding ...ribonuclease E / ribonuclease E activity / tRNA processing / mRNA catabolic process / RNA endonuclease activity / cytoplasmic side of plasma membrane / rRNA processing / tRNA binding / rRNA binding / magnesium ion binding / zinc ion binding / cytoplasm
Similarity search - Function
: / RNase E/G, Thioredoxin-like domain / Ribonuclease E/G / Ribonuclease E / RNA-binding protein AU-1/Ribonuclease E/G / Ribonuclease E/G family / S1 domain profile. / Ribosomal protein S1-like RNA-binding domain / S1 RNA binding domain / S1 domain / Nucleic acid-binding, OB-fold
Similarity search - Domain/homology
Biological speciesBurkholderia pseudomallei (bacteria)
CitationDate: 2019 Dec
Title: A structural and biochemical comparison of Ribonuclease E homologues from pathogenic bacteria highlights species-specific properties
Authors: Mardle C / Shakespeare T / Butt L / Goddard L / Gowers D / Atkins H / Vincent H
Contact author
  • A Callaghan (University of Portsmouth)

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Structure visualization

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Models

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Sample

SampleName: Ribonuclease E from Burkholderia pseudomallei / Specimen concentration: 6.41 mg/ml
BufferName: 10 mM DTT, 10 mM MgCl2, 0.5 M NaCl, 20 mM Tris / pH: 8
Entity #1206Name: RNase E / Type: protein / Description: Endoribonuclease E / Formula weight: 62.584 / Num. of mol.: 4 / Source: Burkholderia pseudomallei / References: UniProt: A0A0H3HN63
Sequence: HHHHHHHHHH SSGHIEGRHM KRMLFNATQQ EELRVAIVDG QKLIDIDIET AGREQRKGNI YKGVVTRIEP SLEACFVNYG EDRHGFLPFK EVARQYFKEG VDMRSARIQD ALREGQELIV QVEKEERGNK GAALTTFISL AGRYLVLMPN NPRGGGVSRR IEGDERQELR ...Sequence:
HHHHHHHHHH SSGHIEGRHM KRMLFNATQQ EELRVAIVDG QKLIDIDIET AGREQRKGNI YKGVVTRIEP SLEACFVNYG EDRHGFLPFK EVARQYFKEG VDMRSARIQD ALREGQELIV QVEKEERGNK GAALTTFISL AGRYLVLMPN NPRGGGVSRR IEGDERQELR ETMAQLQIPD GMSMIARTAG IGRSAEELQW DLNYLLQLWR AIEAASQSGH PGQPMLIYLE SSLVIRAIRD YFQPDIGEIL IDTTEIHDQA RAFMDIVMPD NVGKVKRYHD DVPLFSRFQI EHQIETAYSR TVPLPSGGAI VIDHTEALVA IDVNSARATK GADIEETATR TNLEAADEVA RQLRLRDLGG LIVIDFIDME SAKSQREVEQ RLKDALKHDR ARVQMGKISR FGLMELSRQR LRPALSEGSH VTCPRCNGTG HIRDTESSAL QVLRIIQEEA MKENTAAIHC QVPVEVTAFL LNEKRQEINK IESRFKVGVV LIPNKHLDTP HYKLERLRHD DARLDDPRAS WKMAEEAARE LEAETGYSKR AEETKPRQEA A

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 4.014 mm
DetectorName: Pilatus 2M
Scan
Title: Ribonuclease E from Burkholderia pseudomallei / Measurement date: Feb 11, 2017 / Storage temperature: 21 °C / Cell temperature: 15 °C / Exposure time: 3 sec. / Number of frames: 640 / Unit: 1/A /
MinMax
Q0.004 0.442
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 653 /
MinMax
Q0.00979812 0.167314
P(R) point1 653
R0 149
Result
Type of curve: single_conc /
ExperimentalPorod
MW257 kDa273 kDa
Volume-437 nm3

P(R)GuinierGuinier error
Forward scattering, I00.02609 0.02611 7.0E-5
Radius of gyration, Rg4.82 nm4.826 nm0.02

MinMax
D-14.9
Guinier point42 96

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