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- SASDDT2: EspG3 chaperone from Mycobacterium tuberculosis (EspG3Mtb) -

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Basic information

Entry
Database: SASBDB / ID: SASDDT2
SampleEspG3 chaperone from Mycobacterium tuberculosis
  • EspG3 chaperone from Mycobacterium tuberculosis (protein), EspG3Mtb, Mycobacterium tuberculosis
Function / homologyEspG family / EspG family / cytoplasm / ESX-3 secretion-associated protein EspG3
Function and homology information
Biological speciesMycobacterium tuberculosis (bacteria)
CitationDate: 2018 Jan 25
Title: Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
Authors: Tuukkanen A / Freire D / Chan S / Arbing M / Reed R / Evans T / Zenkeviciutė G / Kim J / Kahng S / Sawaya M / Wilmanns M / Eisenberg D / Parret A
Contact author
  • Anne Tuukkanen (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

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Models

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Sample

SampleName: EspG3 chaperone from Mycobacterium tuberculosis / Specimen concentration: 1.1 mg/ml
BufferName: 20 mM HEPES pH 7.5, 150 mM NaCl / pH: 7.5
Entity #927Name: EspG3Mtb / Type: protein
Description: EspG3 chaperone from Mycobacterium tuberculosis
Formula weight: 33.6 / Source: Mycobacterium tuberculosis / References: UniProt: P9WJC6
Sequence: MHHHHHHENL YFQGSHMDAT PNAVELTVDN AWFIAETIGA GTFPWVLAIT MPYSDAAQRG AFVDRQRDEL TRMGLLSPQG VINPAVADWI KVVCFPDRWL DLRYVGPASA DGACELLRGI VALRTGTGKT SNKTGNGVVA LRNAQLVTFT AMDIDDPRAL VPILGVGLAH ...Sequence:
MHHHHHHENL YFQGSHMDAT PNAVELTVDN AWFIAETIGA GTFPWVLAIT MPYSDAAQRG AFVDRQRDEL TRMGLLSPQG VINPAVADWI KVVCFPDRWL DLRYVGPASA DGACELLRGI VALRTGTGKT SNKTGNGVVA LRNAQLVTFT AMDIDDPRAL VPILGVGLAH RPPARFDEFS LPTRVGARAD ERLRSGVPLG EVVDYLGIPA SARPVVESVF SGPRSYVEIV AGCNRDGRHT TTEVGLSIVD TSAGRVLVSP SRAFDGEWVS TFSPGTPFAI AVAIQTLTAC LPDGQWFPGQ RVSRDFSTQS S

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å
DetectorName: Pilatus 2M
Scan
Title: EspG3 chaperone from Mycobacterium tuberculosis / Measurement date: Mar 17, 2014 / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.1302 4.4504
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 938 /
MinMax
Q0.132787 3.14804
P(R) point1 938
R0 9
Result
Experimental MW: 23 kDa / Type of curve: single_conc
P(R)GuinierGuinier error
Forward scattering, I0579.6 582.72 5.04
Radius of gyration, Rg2.552 nm2.54 nm0.05

MinMax
D-9
Guinier point2 145

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