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- SASDDX2: EspG3-PE5/PPE4 complex from M. tuberculosis (EspG3 chaperone from... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDDX2
SampleEspG3-PE5/PPE4 complex from M. tuberculosis
  • EspG3 chaperone from Mycobacterium tuberculosis (protein), EspG3Mtb, Mycobacterium tuberculosis
  • PE5 from Mycobacterium tuberculosis (protein), PE5, Mycobacterium tuberculosis
  • PPE4 from Mycobacterium tuberculosis (protein), PPE4, Mycobacterium tuberculosis
Function / homology
Function and homology information


response to host immune response / cell envelope / cell surface / extracellular region / plasma membrane / cytoplasm
Similarity search - Function
PPE-PPW subfamily, C-terminal / PPE-PPW subfamily C-terminal region / PPE family / PPE family / PE-PGRS family, N-terminal / PE family / EspG family / EspG family / PPE superfamily
Similarity search - Domain/homology
PE family immunomodulator PE5 / PPE family protein PPE4 / ESX-3 secretion-associated protein EspG3
Similarity search - Component
Biological speciesMycobacterium tuberculosis (bacteria)
CitationDate: 2018 Jan 25
Title: Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
Authors: Tuukkanen A / Freire D / Chan S / Arbing M / Reed R / Evans T / Zenkeviciutė G / Kim J / Kahng S / Sawaya M / Wilmanns M / Eisenberg D / Parret A
Contact author
  • Anne Tuukkanen (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Structure visualization

Downloads & links

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Models

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Sample

SampleName: EspG3-PE5/PPE4 complex from M. tuberculosis / Specimen concentration: 1.22 mg/ml / Entity id: 927 / 941 / 942
BufferName: 20 mM HEPES pH 7.5, 150 mM NaCl / pH: 7.5
Entity #927Name: EspG3Mtb / Type: protein
Description: EspG3 chaperone from Mycobacterium tuberculosis
Formula weight: 33.6 / Source: Mycobacterium tuberculosis / References: UniProt: P9WJC6
Sequence: MHHHHHHENL YFQGSHMDAT PNAVELTVDN AWFIAETIGA GTFPWVLAIT MPYSDAAQRG AFVDRQRDEL TRMGLLSPQG VINPAVADWI KVVCFPDRWL DLRYVGPASA DGACELLRGI VALRTGTGKT SNKTGNGVVA LRNAQLVTFT AMDIDDPRAL VPILGVGLAH ...Sequence:
MHHHHHHENL YFQGSHMDAT PNAVELTVDN AWFIAETIGA GTFPWVLAIT MPYSDAAQRG AFVDRQRDEL TRMGLLSPQG VINPAVADWI KVVCFPDRWL DLRYVGPASA DGACELLRGI VALRTGTGKT SNKTGNGVVA LRNAQLVTFT AMDIDDPRAL VPILGVGLAH RPPARFDEFS LPTRVGARAD ERLRSGVPLG EVVDYLGIPA SARPVVESVF SGPRSYVEIV AGCNRDGRHT TTEVGLSIVD TSAGRVLVSP SRAFDGEWVS TFSPGTPFAI AVAIQTLTAC LPDGQWFPGQ RVSRDFSTQS S
Entity #941Name: PE5 / Type: protein / Description: PE5 from Mycobacterium tuberculosis / Formula weight: 9.6 / Num. of mol.: 1 / Source: Mycobacterium tuberculosis / References: UniProt: L7N695
Sequence:
MTLRVVPEGL AAASAAVEAL TARLAAAHAS AAPVITAVVP PAADPVSLQT AAGFSAQGVE HAVVTAEGVE ELGRAGVGVG ESGASYLAGD AAAAATYGVV GG
Entity #942Name: PPE4 / Type: protein / Description: PPE4 from Mycobacterium tuberculosis / Formula weight: 51.8 / Num. of mol.: 1 / Source: Mycobacterium tuberculosis / References: UniProt: P9WI43
Sequence: MAAPIWMASP PEVHSALLSN GPGPGSLVAA ATAWSQLSAE YASTAAELSG LLGAVPGWAW QGPSAEWYVA AHLPYVAWLT QASADAAGAA AQHEAAAAAY TTALAAMPTL AELAANHVIH TVLVATNFFG INTIPITLNE ADYVRMWLQA AAVMGLYQAA SGAALASAPR ...Sequence:
MAAPIWMASP PEVHSALLSN GPGPGSLVAA ATAWSQLSAE YASTAAELSG LLGAVPGWAW QGPSAEWYVA AHLPYVAWLT QASADAAGAA AQHEAAAAAY TTALAAMPTL AELAANHVIH TVLVATNFFG INTIPITLNE ADYVRMWLQA AAVMGLYQAA SGAALASAPR TVPAPTVMNP GGGAASTVGA VNPWQWLLAL LQQLWNAYTG FYGWMLQLIW QFLQDPIGNS IKIIIAFLTN PIQALITYGP LLFALGYQIF FNLVGWPTWG MILSSPFLLP AGLGLGLAAI AFLPIVLAPA VIPPASTPLA AAAVAAGSVW PAVSMAVTGA GTAGAATPAA GAAPSAGAAP APAAPATASF AYAVGGSGDW GPSLGPTVGG RGGIKAPAAT VPAAAAAAAT RGQSRARRRR RSELRDYGDE FLDMDSDSGF GPSTGDHGAQ ASERGAGTLG FAGTATKERR VRAVGLTALA GDEFGNGPRM PMVPGTWEQG SNEPEAPDGS GRGGGDGLPH DSK

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Experimental information

BeamInstrument name: Diamond Light Source B21 / City: Oxfordshire / 国: UK / Shape: 1 x 5 mm / Type of source: X-ray synchrotron
DetectorName: Pilatus 2M
Scan
Title: EspG3-PE5/PPE4 complex from M. tuberculosis / Measurement date: Jun 15, 2015 / Unit: 1/nm /
MinMax
Q0.0834 4.0114
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 698 /
MinMax
Q0.105427 2.02128
P(R) point1 698
R0 14.2
Result
Experimental MW: 83 kDa / Type of curve: single_conc
P(R)GuinierGuinier error
Forward scattering, I00.003034 0.00299 0.00015
Radius of gyration, Rg4.197 nm3.98 nm0.03

MinMax
D-14.2
Guinier point9 90

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