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- SASDDU2: EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled) -

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Basic information

Entry
Database: SASBDB / ID: SASDDU2
SampleEspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)
  • EspG3 chaperone from Mycobacterium smegmatis (protein), EspG3Msm, Mycobacterium smegmatis
Function / homologyEspG family / EspG family / cytoplasm / ESX-3 secretion-associated protein EspG3
Function and homology information
Biological speciesMycobacterium smegmatis (bacteria)
CitationDate: 2018 Jan 25
Title: Structural variability of EspG chaperones from mycobacterial ESX-1, ESX-3 and ESX-5 type VII secretion systems
Authors: Tuukkanen A / Freire D / Chan S / Arbing M / Reed R / Evans T / Zenkeviciutė G / Kim J / Kahng S / Sawaya M / Wilmanns M / Eisenberg D / Parret A
Contact author
  • Anne Tuukkanen (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

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Models

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Sample

SampleName: EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)
Specimen concentration: 3.79 mg/ml
BufferName: 20 mM HEPES pH 7.5, 150 mM NaCl / pH: 7.5
Entity #926Name: EspG3Msm / Type: protein / Description: EspG3 chaperone from Mycobacterium smegmatis / Formula weight: 31.8 / Num. of mol.: 1 / Source: Mycobacterium smegmatis / References: UniProt: A0QQ45
Sequence: GHMGPNAVEL TTDQAWCLAD VLGAGSYPWV LAITPPYSDH SQRSAFLAAQ SAELTRMGVV NSAGAVDPRV AQWITTVCRA TQWLDLRFVS GPGDLLRGMV ARRSEETVVA LRNAQLVTFT AMDIGHQHAL VPVLTAGLSG RKPARFDDFA LPAAAGARAD EQIRNGAPLA ...Sequence:
GHMGPNAVEL TTDQAWCLAD VLGAGSYPWV LAITPPYSDH SQRSAFLAAQ SAELTRMGVV NSAGAVDPRV AQWITTVCRA TQWLDLRFVS GPGDLLRGMV ARRSEETVVA LRNAQLVTFT AMDIGHQHAL VPVLTAGLSG RKPARFDDFA LPAAAGARAD EQIRNGAPLA EVLEFLGVPP SARPLVESVF DGRRTYVEIV AGEHRDGHRV TTEVGVSIID TPHGRILVHP TKAFDGEWIS TFTPGSADAI AMAVERLTAS LPSGSWFPDQ PLTRDFDEDA ATHREPVLQR RTQKA

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron / Wavelength: 0.124 Å
DetectorName: Pilatus 2M
Scan
Title: EspG3 chaperone from Mycobacterium smegmatis (Sel-Met labelled)
Measurement date: Mar 17, 2014 / Exposure time: 0.05 sec. / Number of frames: 20 / Unit: 1/nm /
MinMax
Q0.0779 4.0114
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1089 /
MinMax
Q0.0889351 3.07953
P(R) point1 1089
R0 9.2
Result
Experimental MW: 24.3 kDa / Type of curve: single_conc
P(R)GuinierGuinier error
Forward scattering, I00.001931 0.001932 3.2E-6
Radius of gyration, Rg2.623 nm2.6 nm0.06

MinMax
D-9.2
Guinier point5 150

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