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- SASDCS6: Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS ... -

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ID or keywords:

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Basic information

Entry
Database: SASBDB / ID: SASDCS6
SampleRap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP
  • Rap guanine nucleotide exchange factor 3 (protein), EPAC1B, Homo sapiens
  • RAS related protein 1b (protein), Rap1b, Mus musculus
Function / homology
Function and homology information


cellular response to gonadotropin-releasing hormone / cellular response to xenobiotic stimulus => GO:0071466 / Rap protein signal transduction / regulation of cell junction assembly / positive regulation of integrin activation / response to carbohydrate / establishment of endothelial barrier / regulation of establishment of cell polarity / cellular response to cAMP / lipid droplet ...cellular response to gonadotropin-releasing hormone / cellular response to xenobiotic stimulus => GO:0071466 / Rap protein signal transduction / regulation of cell junction assembly / positive regulation of integrin activation / response to carbohydrate / establishment of endothelial barrier / regulation of establishment of cell polarity / cellular response to cAMP / lipid droplet / GDP binding / cell-cell junction / GTPase activity / protein-containing complex binding / GTP binding / membrane / cytosol
Similarity search - Function
Ras-related protein Rap1 / Small GTPase, Ras-type / small GTPase Ras family profile. / Small GTPase / Ras family / Small GTP-binding protein domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Isoform 3 of Rap guanine nucleotide exchange factor 3 / :
Similarity search - Component
Biological speciesHomo sapiens (human)
Mus musculus (house mouse)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Models

Model #1144
Type: atomic / Radius of dummy atoms: 1.90 A / Symmetry: P1 / Comment: Combined remodelled 3CF6 with 4F7Z and SwissModel / Chi-square value: 0.81 / P-value: 0.022800
Search similar-shape structures of this assembly by Omokage search (details)
Model #1145
Type: dummy / Radius of dummy atoms: 3.75 A / Symmetry: P1 / Comment: SAXNS_DAMMIF / Chi-square value: 0.588289 / P-value: 0.008000
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP
Specimen concentration: 1.00-4.00 / Entity id: 595 / 596
BufferName: 1mM EDTA, 10mM DTT, 500mM NaCl, 1mM cAMP, and 10mM Tris
pH: 9
Entity #595Name: EPAC1B / Type: protein / Description: Rap guanine nucleotide exchange factor 3 / Formula weight: 100.001 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: O95398-3
Sequence: GSPGIRVLVL RRMHRPRSCS YQLLLEHQRP SCIQGLRWTP LTNSEESLDF SESLEQASTE RVLRAGRQLH RHLLATCPNL IRDRKYHLRL YRQCCSGREL VDGILALGLG VHSRSQVVGI CQVLLDEGAL CHVKHDWAFQ DRDAQFYRFP GPEPEPVGTH EMEEELAEAV ...Sequence:
GSPGIRVLVL RRMHRPRSCS YQLLLEHQRP SCIQGLRWTP LTNSEESLDF SESLEQASTE RVLRAGRQLH RHLLATCPNL IRDRKYHLRL YRQCCSGREL VDGILALGLG VHSRSQVVGI CQVLLDEGAL CHVKHDWAFQ DRDAQFYRFP GPEPEPVGTH EMEEELAEAV ALLSQRGPDA LLTVALRKPP GQRTDEELDL IFEELLHIKA VAHLSNSVKR ELAAVLLFEP HSKAGTVLFS QGDKGTSWYI IWKGSVNVVT HGKGLVTTLH EGDDFGQLAL VNDAPRAATI ILREDNCHFL RVDKQDFNRI IKDVEAKTMR LEEHGKVVLV LERASQGAGP SRPPTPGRNR YTVMSGTPEK ILELLLEAMG PDSSAHDPTE TFLSDFLLTH RVFMPSAQLC AALLHHFHVE PAGGSEQERS TYVCNKRQQI LRLVSQWVAL YGSMLHTDPV ATSFLQKLSD LVGRDTRLSN LLREQWPERR RCHRLENGCG NASPQMKARN LPVWLPNQDE PLPGSSCAIQ VGDKVPYDIC RPDHSVLTLQ LPVTASVREV MAALAQEDGW TKGQVLVKVN SAGDAIGLQP DARGVATSLG LNERLFVVNP QEVHELIPHP DQLGPTVGSA EGLDLVSAKD LAGQLTDHDW SLFNSIHQVE LIHYVLGPQH LRDVTTANLE RFMRRFNELQ YWVATELCLC PVPGPRAQLL RKFIKLAAHL KEQKNLNSFF AVMFGLSNSA ISRLAHTWER LPHKVRKLYS ALERLLDPSW NHRVYRLALA KLSPPVIPFM PLLLKDMTFI HEGNHTLVEN LINFEKMRMM ARAARMLHHC RSHNPVPLSP LRSRVSHLHE DSQVARISTC SEQSLSTRSP ASTWAYVQQL KVIDNQRELS RLSRELEP
Entity #596Name: Rap1b / Type: protein / Description: RAS related protein 1b / Formula weight: 18 / Num. of mol.: 1 / Source: Mus musculus / References: UniProt: q52l50
Sequence:
MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDAQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTDDVP MILVGNKCDL EDERVVGKEQ GQNLARQWNN CAFLESSAKS KINVNEIFYD LVRQINR

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Experimental information

BeamInstrument name: Sealy Center For Structural Biology, UTMB-G Rigaku BioSAXS-1000
City: Galveston, TX / 国: USA / Type of source: X-ray in house / Wavelength: 0.15418 Å / Dist. spec. to detc.: 0.489 mm
DetectorName: Rigaku BioSAXS-1000 / Type: Kratky-2D / Pixsize x: 0.001 mm
Scan
Title: Rap guanine nucleotide exchange factor 3 (isoform3) bound to RAS related protein 1b - with cAMP
Measurement date: Apr 1, 2013 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1800 sec. / Number of frames: 5 / Unit: 1/A /
MinMax
Q0.0123 0.6788
Distance distribution function P(R)
Sofotware P(R): GNOM 4.5a / Number of points: 129 /
MinMax
Q0.0137 0.1964
P(R) point2 130
R0 141.7
Result
Type of curve: single_conc /
ExperimentalPorod
MW119 kDa124 kDa
Volume-207 nm3

P(R)P(R) errorGuinierGuinier error
Forward scattering, I087670 763 85780 1523
Radius of gyration, Rg4.27 nm0.03 4.05 nm0.09

MinMax
D-14.2
Guinier point2 14

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