SASDCN7: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A ...

基本情報

登録情報

データベース: SASBDB / ID: SASDCN7

試料

Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein

• Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein (protein), Mus musculus

• 生物種: Mus musculus (ハツカネズミ)
• 引用: ジャーナル: Structure / 年: 2018; タイトル: Structural Dynamics Control Allosteric Activation of Cytohesin Family Arf GTPase Exchange Factors.; 著者: Andrew W Malaby / Sanchaita Das / Srinivas Chakravarthy / Thomas C Irving / Osman Bilsel / David G Lambright / ; 要旨: Membrane dynamic processes including vesicle biogenesis depend on Arf guanosine triphosphatase (GTPase) activation by guanine nucleotide exchange factors (GEFs) containing a catalytic Sec7 domain and a membrane-targeting module such as a pleckstrin homology (PH) domain. The catalytic output of cytohesin family Arf GEFs is controlled by autoinhibitory interactions that impede accessibility of the exchange site in the Sec7 domain. These restraints can be relieved through activator Arf-GTP binding to an allosteric site comprising the PH domain and proximal autoinhibitory elements (Sec7-PH linker and C-terminal helix). Small-angle X-ray scattering and negative-stain electron microscopy were used to investigate the structural organization and conformational dynamics of cytohesin-3 (Grp1) in autoinhibited and active states. The results support a model in which hinge dynamics in the autoinhibited state expose the activator site for Arf-GTP binding, while subsequent C-terminal helix unlatching and repositioning unleash conformational entropy in the Sec7-PH linker to drive exposure of the exchange site.

登録者

• David Lambright (UMASS Medical School)

モデル

試料

• 試料: 名称: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein; 試料濃度: 2.4 mg/ml
• バッファ: 名称: 20 mM Tris, 150 mM NaCl, 2 mM MgCl2, 0.1% 2-mercaptoethanol, 5% glycerol, 0.001 mM insitol 1,3,4,5-tetrakis phosphate; pH: 8

要素 #796

タイプ: protein / 記述: Grp1 63-399 E161A 6GS Arf6 Q67L His fusion protein / 分子量: 61.834 / 分子数: 1 / 由来: Mus musculus
配列:

MGHHHHHHGS TTQRNAQIAM GRKKFNMDPK KGIQFLIEND LLQSSPEDVA QFLYKGEGLN KTVIGDYLGE RDDFNIKVLQ AFVELHEFAD LNLVQALRQF LWSFRLPGEA QKIDRMMEAF ASRYCLCNPG VFQSTDTCYV LSFAIIMLNT SLHNHNVRDK PTAERFITMN RGINEGGDLP EELLRNLYES IKNEPFKIPE DDGNDLTHTF FNPDREGWLL KLGGRVKTWK RRWFILTDNC LYYFEYTTDK EPRGIIPLEN LSIREVEDPR KPNCFELYNP SHKGQVIKAC KTEADGRVVE GNHVVYRISA PSPEEKEEWM KSIKASISRD PFYDMLATRK RRIANKKGSG SGSGSGSGSG KVLSKIFGNK EMRILMLGLD AAGKTTILYK LKLGQSVTTI PTVGFNVETV TYKNVKFNVW DVGGLDKIRP LWRHYYTGTQ GLIFVVDCAD RDRIDEARQE LHRIINDREM RDAIILIFAN KQDLPDAMKP HEIQEKLGLT RIRDRNWYVQ PSCATSGDGL YEGLTWLTSN YNHHHHHH

実験情報

• ビーム: 設備名称: Advanced Photon Source (APS) BioCAT 18ID / 地域: Argonne, IL / 国: USA / 線源: X-ray synchrotron / 波長: 0.10332 Å / スペクトロメータ・検出器間距離: 2.5 mm
• 検出器: 名称: MAR 165 CCD

スキャン

タイトル: Truncated monomeric Cytohesin-3 (Grp1; amino acids 63-399) E161A 6GS Arf6 Q67L His6 fusion protein
測定日: 2013年11月15日 / セル温度: 20 °C / 照射時間: 1 sec. / フレーム数: 1 / 単位: 1/A /

	Min	Max
Q	0.0069	0.3162

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 76 /

	Min	Max
Q	0.00688375	0.316224
P(R) point	1	76
R	0	136

結果

カーブのタイプ: other /

	実験値	Porod
分子量	67 kDa	67 kDa
体積	-	100.5 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	1.01	0.001	1.01	0.001
慣性半径, Rg	3.43 nm	0.01	3.37 nm	0.008

	Min	Max	Error
D	-	13.6	0.1
Guinier point	1	29	-