SASDCD2: Rab family protein CtRoc-COR GppNHp (Rab family protein)

基本情報

登録情報

データベース: SASBDB / ID: SASDCD2

試料

Rab family protein CtRoc-COR GppNHp

• Rab family protein (protein), Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)

機能・相同性

分子機能:

GTP binding / identical protein binding

ドメイン・相同性:

C-terminal of Roc (COR) domain / C-terminal of Roc, COR, domain / Leucine rich repeat 4 / Leucine Rich repeats (2 copies) / Roc domain profile. / Roc domain / Leucine-rich repeat, typical subtype / Leucine-rich repeats, typical (most populated) subfamily / Leucine-rich repeat profile. / ロイシンリッチリピート / Leucine-rich repeat domain superfamily / Small GTP-binding protein domain / Winged helix-like DNA-binding domain superfamily / P-loop containing nucleoside triphosphate hydrolase

構成要素:

Rab family protein

• 生物種: Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS) (バクテリア)
• 引用: ジャーナル: Nat Commun / 年: 2017; タイトル: A homologue of the Parkinson's disease-associated protein LRRK2 undergoes a monomer-dimer transition during GTP turnover.; 著者: Egon Deyaert / Lina Wauters / Giambattista Guaitoli / Albert Konijnenberg / Margaux Leemans / Susanne Terheyden / Arsen Petrovic / Rodrigo Gallardo / Laura M Nederveen-Schippers / Panagiotis S Athanasopoulos / Henderikus Pots / Peter J M Van Haastert / Frank Sobott / Christian Johannes Gloeckner / Rouslan Efremov / Arjan Kortholt / Wim Versées / ; 要旨: Mutations in LRRK2 are a common cause of genetic Parkinson's disease (PD). LRRK2 is a multi-domain Roco protein, harbouring kinase and GTPase activity. In analogy with a bacterial homologue, LRRK2 was proposed to act as a GTPase activated by dimerization (GAD), while recent reports suggest LRRK2 to exist under a monomeric and dimeric form in vivo. It is however unknown how LRRK2 oligomerization is regulated. Here, we show that oligomerization of a homologous bacterial Roco protein depends on the nucleotide load. The protein is mainly dimeric in the nucleotide-free and GDP-bound states, while it forms monomers upon GTP binding, leading to a monomer-dimer cycle during GTP hydrolysis. An analogue of a PD-associated mutation stabilizes the dimer and decreases the GTPase activity. This work thus provides insights into the conformational cycle of Roco proteins and suggests a link between oligomerization and disease-associated mutations in LRRK2.

登録者

• Egon Deyaert (VUB, Vrije Universiteit Brussel, Pleinlaan 2 1050 Brussel)

モデル

試料

• 試料: 名称: Rab family protein CtRoc-COR GppNHp / 試料濃度: 1.00-2.00
• バッファ: 名称: 20 mM HEPES 150 mM NaCl 5 mM MgCl2 5% Glycerol 1 mM DTT 200µM GppNHp; pH: 7.5

要素 #605

タイプ: protein / 記述: Rab family protein / 分子量: 64.865 / 分子数: 2
由来: Chlorobium tepidum (strain ATCC 49652 / DSM 12025 / NBRC 103806 / TLS)
参照: UniProt: Q8KC98_CHL
配列:

MSYYHHHHHH DYDIPTTENL YFQGAMGSDN PLESPPPEIV KQGKEAVRQY FQSIEEARSK GEALVHLQEI KVHLIGDGMA GKTSLLKQLI GETFDPKESQ THGLNVVTKQ APNIKGLEND DELKECLFHF WDFGGQEIMH ASHQFFMTRS SVYMLLLDSR TDSNKHYWLR HIEKYGGKSP VIVVMNKIDE NPSYNIEQKK INERFPAIEN RFHRISCKNG DGVESIAKSL KSAVLHPDSI YGTPLAPSWI KVKEKLVEAT TAQRYLNRTE VEKICNDSGI TDPGERKTLL GYLNNLGIVL YFEALDLSEI YVLDPHWVTI GVYRIINSSK TKNGHLNTSA LGYILNEEQI RCDEYDPAKN NKFTYTLLEQ RYLLDIMKQF ELCYDEGKGL FIIPSNLPTQ IDNEPEITEG EPLRFIMKYD YLPSTIIPRL MIAMQHQILD RMQWRYGMVL KSQDHEGALA KVVAETKDST ITIAIQGEPR CKREYLSIIW YEIKKINANF TNLDVKEFIP LPGHPDELVE YKELLGLEKM GRDEYVSGKL EKVFSVSKML DSVISKEERN KER

実験情報

• ビーム: 設備名称: SOLEIL SWING / 地域: Saint-Aubin / 国: France / 線源: X-ray synchrotronシンクロトロン / 波長: 0.103 Å / スペクトロメータ・検出器間距離: 25 mm
• 検出器: 名称: AVIEX / タイプ: CCD

スキャン

タイトル: Rab family protein CtRoc-COR GppNHp / 測定日: 2017年2月1日 / 照射時間: 0.75 sec. / フレーム数: 31 / 単位: 1/A /

	Min	Max
Q	0.004	0.6134

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.6 / ポイント数: 475 /

	Min	Max
Q	0.01819	0.2875
P(R) point	26	500
R	0	105

結果

カーブのタイプ: other /

	実験値	Porod
分子量	78 kDa	90 kDa
体積	-	153.69 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.06889	3.0E-5	0.069238	5.0E-5
慣性半径, Rg	3.331 nm	0.002	3.36 nm	0.003

	Min	Max
D	-	10.5
Guinier point	26	61