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- SASDCC7: Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactiv... -

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Basic information

Entry
Database: SASBDB / ID: SASDCC7
SampleThermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)
  • T.maritima PDE (protein), TmPDE D80N D154N, Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
Function / homologyDDH domain / DHH family / DHH phosphoesterase superfamily / DHHA1 domain / DHHA1 domain / nucleic acid binding / Uncharacterized protein
Function and homology information
Biological speciesThermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099) (bacteria)
CitationDate: 2017 Oct
Title: Structural and Biophysical Analysis of the Soluble DHH/DHHA1-Type Phosphodiesterase TM1595 from Thermotoga maritima
Authors: Drexler D / Müller M / Rojas-Cordova C / Bandera A
Contact author
  • Gregor Witte (Ludwig-Maximilians-Universität München)

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Structure visualization

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Models

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Sample

SampleName: Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)
BufferName: 25mM Tris 500mM NaCl 3% (v/v) glycerol 2mM MgCl2 / pH: 8
Entity #758Name: TmPDE D80N D154N / Type: protein / Description: T.maritima PDE / Formula weight: 37.821 / Num. of mol.: 2
Source: Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
References: UniProt: Q9X1T1
Sequence: GSGSGMDEIV KVLSQHDRIL VVGHIMPDGD CVSSVLSLTL GLEKLGKEVK AAVDYKIPYV FEKFPYIDKI EENPNFDPEL LVVVNASSPD RIGKFQDLLD KVPSVVIDHH STNTNFGNWN WVDPSFAATA QMIFRINKAL GVEYDSNLAT LNYLGIATNT GFFRHSNADV ...Sequence:
GSGSGMDEIV KVLSQHDRIL VVGHIMPDGD CVSSVLSLTL GLEKLGKEVK AAVDYKIPYV FEKFPYIDKI EENPNFDPEL LVVVNASSPD RIGKFQDLLD KVPSVVIDHH STNTNFGNWN WVDPSFAATA QMIFRINKAL GVEYDSNLAT LNYLGIATNT GFFRHSNADV RVFEDAYKLV KMGADAHFVA KEILENKRFE QFKLFAEVLE RLQLLENGKI AYSYIDYDTY LRHNCTDEDS AGFVGELRSI RGVEVAVLFM EFPRGKIHVS MRSKDWFNVN EVAFELGGGG HPRAAGVTFE GKKIEEVIPR VINHLLKKFK EGVESESEKI PEGDVLGG

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Experimental information

BeamInstrument name: PETRA III P12 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.124 Å / Dist. spec. to detc.: 3 mm
DetectorName: Pilatus 2M
Scan
Title: Thermotoga martitima phosphodiesterase TM1595 D80N D154N (inactive mutant)
Measurement date: Jun 17, 2016 / Storage temperature: 20 °C / Cell temperature: 20 °C / Exposure time: 0.995 sec. / Unit: 1/nm /
MinMax
Q0 2.9267
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1310 /
MinMax
Q0.213746 2.92667
P(R) point1 1310
R0 7.86
Result
Type of curve: single_conc /
ExperimentalPorod
MW72 kDa69.2 kDa
Volume-115.38 nm3

P(R)GuinierGuinier error
Forward scattering, I09314 9293.71 6.68
Radius of gyration, Rg2.78 nm2.79 nm0.03

MinMax
D-7.9
Guinier point54 175

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