+データを開く
-基本情報
登録情報 | データベース: SASBDB / ID: SASDC46 |
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試料 | PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210
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機能・相同性 | 機能・相同性情報 negative regulation of respiratory burst / negative regulation of cellular extravasation / negative regulation of macrophage migration / negative regulation of blood vessel remodeling / negative regulation of neutrophil degranulation / macrophage migration / neutrophil degranulation / intracellular protein transmembrane transport / renal system process / regulation of vascular permeability ...negative regulation of respiratory burst / negative regulation of cellular extravasation / negative regulation of macrophage migration / negative regulation of blood vessel remodeling / negative regulation of neutrophil degranulation / macrophage migration / neutrophil degranulation / intracellular protein transmembrane transport / renal system process / regulation of vascular permeability / regulation of Rho protein signal transduction / focal adhesion assembly / definitive hemopoiesis / Signaling by cytosolic FGFR1 fusion mutants / activation of GTPase activity / regulation of small GTPase mediated signal transduction / inner ear morphogenesis / RHOB GTPase cycle / small GTPase-mediated signal transduction / RHOC GTPase cycle / CDC42 GTPase cycle / neuromuscular process controlling balance / homeostasis of number of cells / RHOA GTPase cycle / negative regulation of reactive oxygen species metabolic process / RAC2 GTPase cycle / RAC3 GTPase cycle / phagocytosis / positive regulation of phagocytosis / keratinocyte differentiation / RAC1 GTPase cycle / Signaling by FGFR1 in disease / GTPase activator activity / guanyl-nucleotide exchange factor activity / negative regulation of inflammatory response / modulation of chemical synaptic transmission / brain development / Schaffer collateral - CA1 synapse / protein tyrosine kinase activity / actin cytoskeleton organization / cellular response to lipopolysaccharide / dendritic spine / postsynaptic density / regulation of cell cycle / non-specific serine/threonine protein kinase / protein phosphorylation / axon / protein serine kinase activity / protein serine/threonine kinase activity / glutamatergic synapse / signal transduction / protein-containing complex / extracellular exosome / ATP binding / membrane / plasma membrane / cytosol 類似検索 - 分子機能 |
生物種 | Homo sapiens (ヒト) |
引用 | ジャーナル: Nat Commun / 年: 2017 タイトル: Structural and functional dissection of the DH and PH domains of oncogenic Bcr-Abl tyrosine kinase. 著者: Sina Reckel / Charlotte Gehin / Delphine Tardivon / Sandrine Georgeon / Tim Kükenshöner / Frank Löhr / Akiko Koide / Lena Buchner / Alejandro Panjkovich / Aline Reynaud / Sara Pinho / ...著者: Sina Reckel / Charlotte Gehin / Delphine Tardivon / Sandrine Georgeon / Tim Kükenshöner / Frank Löhr / Akiko Koide / Lena Buchner / Alejandro Panjkovich / Aline Reynaud / Sara Pinho / Barbara Gerig / Dmitri Svergun / Florence Pojer / Peter Güntert / Volker Dötsch / Shohei Koide / Anne-Claude Gavin / Oliver Hantschel / 要旨: The two isoforms of the Bcr-Abl tyrosine kinase, p210 and p190, are associated with different leukemias and have a dramatically different signaling network, despite similar kinase activity. To ...The two isoforms of the Bcr-Abl tyrosine kinase, p210 and p190, are associated with different leukemias and have a dramatically different signaling network, despite similar kinase activity. To provide a molecular rationale for these observations, we study the Dbl-homology (DH) and Pleckstrin-homology (PH) domains of Bcr-Abl p210, which constitute the only structural differences to p190. Here we report high-resolution structures of the DH and PH domains and characterize conformations of the DH-PH unit in solution. Our structural and functional analyses show no evidence that the DH domain acts as a guanine nucleotide exchange factor, whereas the PH domain binds to various phosphatidylinositol-phosphates. PH-domain mutants alter subcellular localization and result in decreased interactions with p210-selective interaction partners. Hence, the PH domain, but not the DH domain, plays an important role in the formation of the differential p210 and p190 Bcr-Abl signaling networks. |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-Data source
SASBDBのページ | SASDC46 |
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-関連構造データ
-外部リンク
「今月の分子」の関連する項目 |
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-モデル
モデル #1387 | タイプ: atomic / ソフトウェア: (2.8.1) / ダミー原子の半径: 1.90 A / カイ2乗値: 1.062 Omokage検索でこの集合体の類似形状データを探す (詳細) |
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-試料
試料 | 名称: PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210 試料濃度: 1.00-9.14 |
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バッファ | 名称: 25 mM Tris-HCl, 150 mM NaCl, 5% Glycerol, 1 mM DTT / pH: 7.5 |
要素 #740 | タイプ: protein / 記述: BCR-ABL p210 fusion protein (PH domain) / 分子量: 22.413 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: P11274 配列: GAMGEHRQLL KDSFMVELVE GARKLRHVFL FTDLLLCTKL KKQSGGKTQQ YDCKWYIPLT DLSFQMVDEL EAVPNIPLVP DEELDALKIK ISQIKNDIQR EKRANKGSKA TERLKKKLSE QESLLLLMSP SMAFRVHSRN GKSYTFLISS DYERAEWREN IREQQKKCFR SFSLTSVELQ MLTNSCVKLQ TVH |
-実験情報
ビーム | 設備名称: PETRA III P12 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron / 波長: 0.124 Å / スペクトロメータ・検出器間距離: 3 mm | ||||||||||||||||||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 2M | ||||||||||||||||||||||||||||||||||||||||||
スキャン | タイトル: PH - Pleckstrin-homology domain of Bcr-Abl tyrosine kinase p210 測定日: 2016年11月10日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 0.045 sec. / フレーム数: 20 / 単位: 1/A /
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距離分布関数 P(R) | ソフトウェア P(R): GNOM 5.0 / ポイント数: 1500 /
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結果 | カーブのタイプ: merged コメント: The homology model is derived from PDB ID: 2z0q. The PDB entry for BCR-ABL p210 fusion protein PH domain is 5oc7 (has an internal deletion).
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