SASDC22: Tandem LIM domains of the neuronal transcription factor homeobox ...

基本情報

• 登録情報: データベース: SASBDB / ID: SASDC22

試料

Tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7

• Homeobox protein CEH-14 (protein), CEH-14, Caenorhabditis elegans
• CeLIM-7 (protein), CeLIM-7, Caenorhabditis elegans

機能・相同性

分子機能:

excretion / pharynx development / gonad morphogenesis / body morphogenesis / nematode larval development / thermosensory behavior / neuron fate specification / head morphogenesis / locomotion / neuron development / cis-regulatory region sequence-specific DNA binding / axonogenesis / RNA polymerase II transcription regulatory region sequence-specific DNA binding / neuron differentiation / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor activity, RNA polymerase II-specific / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / nucleus / metal ion binding

ドメイン・相同性:

LIM zinc-binding domain signature. / LIM domain / Zinc-binding domain present in Lin-11, Isl-1, Mec-3. / Zinc finger, LIM-type / LIM domain profile. / Homeobox, conserved site / 'Homeobox' domain signature. / Homeodomain / 'Homeobox' domain profile. / Homeodomain / Homeobox domain / Homeobox-like domain superfamily

構成要素:

LIM/homeobox protein lim-7 / LIM/homeobox protein ceh-14

• 生物種: Caenorhabditis elegans (センチュウ)
• 引用: ジャーナル: Sci Rep / 年: 2017; タイトル: Interactions between LHX3- and ISL1-family LIM-homeodomain transcription factors are conserved in Caenorhabditis elegans.; 著者: Mugdha Bhati / Estelle Llamosas / David A Jacques / Cy M Jeffries / Siavoush Dastmalchi / Nina Ripin / Hannah R Nicholas / Jacqueline M Matthews / ; 要旨: LIM-Homeodomain (LIM-HD) transcription factors are highly conserved in animals where they are thought to act in a transcriptional 'LIM code' that specifies cell types, particularly in the central nervous system. In chick and mammals the interaction between two LIM-HD proteins, LHX3 and Islet1 (ISL1), is essential for the development of motor neurons. Using yeast two-hybrid analysis we showed that the Caenorhabditis elegans orthologs of LHX3 and ISL1, CEH-14 and LIM-7 can physically interact. Structural characterisation of a complex comprising the LIM domains from CEH-14 and a LIM-interaction domain from LIM-7 showed that these nematode proteins assemble to form a structure that closely resembles that of their vertebrate counterparts. However, mutagenic analysis across the interface indicates some differences in the mechanisms of binding. We also demonstrate, using fluorescent reporter constructs, that the two C. elegans proteins are co-expressed in a small subset of neurons. These data show that the propensity for LHX3 and Islet proteins to interact is conserved from C. elegans to mammals, raising the possibility that orthologous cell specific LIM-HD-containing transcription factor complexes play similar roles in the development of neuronal cells across diverse species.

登録者

• Cy M Jeffries (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #1139: ; タイプ: dummy / ダミー原子の半径: 2.25 A / カイ2乗値: 0.288 / P-value: 0.190200; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1140: ; タイプ: dummy / ダミー原子の半径: 2.25 A / カイ2乗値: 0.288 / P-value: 0.190200; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1141: ; タイプ: dummy / ダミー原子の半径: 1.90 A / カイ2乗値: 0.29 / P-value: 0.025700; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #1142: ; タイプ: atomic / ダミー原子の半径: 1.90 A / カイ2乗値: 0.295 / P-value: 0.192000; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7; 試料濃度: 5.2 mg/ml / Entity id: 593 / 594
• バッファ: 名称: 50 mM Tris, 100 mM NaCl, 5 mM TCEP, / pH: 8

要素 #593

名称: CEH-14 / タイプ: protein / 記述: Homeobox protein CEH-14 / 分子量: 15.693 / 分子数: 1 / 由来: Caenorhabditis elegans / 参照: UniProt: P20271
配列:

GSNNEEAICS LCDKKIRDRF VSKVNGRCYH SSCLRCSTCK DELGATCFLR EDSMYCRAHF YKKFGTKCSS CNEGIVPDHV VRKASNHVYH VECFQCFICK RSLETGEEFY LIADDARLVC KDDYEQARDG GSGGHMGSGG

要素 #594

名称: CeLIM-7 / タイプ: protein / 記述: CeLIM-7 / 分子量: 3.501 / 分子数: 1 / 由来: Caenorhabditis elegans / 参照: UniProt: G5EC36
配列:

GIGPLMVQPA TPHIDNTLGG PIDIQHFAQW EF

実験情報

• ビーム: 設備名称: University of Sydney Anton Paar SAXSess / 地域: Sydney / 国: Australia / 形状: Line collimation / 線源: X-ray in house / 波長: 0.1542 Å / スペクトロメータ・検出器間距離: 0.309 mm
• 検出器: 名称: Roper Scientific PI-SCX:4300 / タイプ: KAF 2084 x 2084 SCX CCD / Pixsize x: 24 mm

スキャン

タイトル: Tandem LIM domains of the neuronal transcription factor homeobox protein CEH-14 fused to the LIM interaction domain of ceLIM-7
測定日: 2009年4月7日 / セル温度: 10 °C / 照射時間: 900 sec. / フレーム数: 4 / 単位: 1/nm /

	Min	Max
Q	0.0674	3.3112

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 439 /

	Min	Max
Q	0.067406	3.31124
P(R) point	1	439
R	0	8.9

結果

カーブのタイプ: single_conc
コメント: The SAXS data displayed for this entry includes the beam-geometry corrected scattering profile (desmeared data, top, and homology model fit) as well as the fit of the ab initio models to the smeared SAXS data (i.e., smeared model-data fits). The original smeared data, prepared experimental beam profile and all individual DAMMIF and Gasbor models are included in the full entry zip archive.

	実験値	Standard	Standard error	Porod
分子量	17 kDa	16.7 kDa	0.9	16 kDa
体積	-	-	-	25.6 nm3

	P(R)	P(R) error	Guinier	Guinier error
前方散乱 I0	0.0833	0.002	0.083	0.0002
慣性半径, Rg	2.51 nm	0.09	2.4 nm	0.03

	Min	Max
D	-	8.9
Guinier point	1	61