SASDBV2: Zebrafish Arpin (Zebrafish (Danio rerio) Arpin, Arpin)

基本情報

登録情報

データベース: SASBDB / ID: SASDBV2

試料

Zebrafish Arpin

• Zebrafish (Danio rerio) Arpin (protein), Arpin, Danio rerio

• 機能・相同性: Arpin / Arp2/3-interacting proteins Arpin / negative regulation of actin nucleation / directional locomotion / negative regulation of cell migration / lamellipodium / Arpin
• 生物種: Danio rerio (ゼブラフィッシュ)
• 引用: ジャーナル: Structure / 年: 2016; タイトル: Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.; 著者: Susan Fetics / Aurélien Thureau / Valérie Campanacci / Magali Aumont-Nicaise / Irène Dang / Alexis Gautreau / Javier Pérez / Jacqueline Cherfils / ; 要旨: Arpin is a newly discovered regulator of actin polymerization at the cell leading edge, which steers cell migration by exerting a negative control on the Arp2/3 complex. Arpin proteins have an acidic tail homologous to the acidic motif of the VCA domain of nucleation-promoting factors (NPFs). This tail is predicted to compete with the VCA of NPFs for binding to the Arp2/3 complex, thereby mitigating activation and/or tethering of the complex to sites of actin branching. Here, we investigated the structure of full-length Arpin using synchrotron small-angle X-ray scattering, and of its acidic tail in complex with an ankyrin repeats domain using X-ray crystallography. The data were combined in a hybrid model in which the acidic tail extends from the globular core as a linear peptide and forms a primary epitope that is readily accessible in unbound Arpin and suffices to tether Arpin to interacting proteins with high affinity.

登録者

• Aurélien Thureau (Soleil, Soleil Synchrotron, Saint-Aubin, France)

モデル

試料

• 試料: 名称: Zebrafish Arpin
• バッファ: 名称: 50 mM HEPES 100mM NaCl 1mM TCEP / 濃度: 50.00 mM / pH: 7.5 / 組成: 100mM NaCl, 1mM TCEP

要素 #276

名称: Arpin / タイプ: protein / 記述: Zebrafish (Danio rerio) Arpin / 分子量: 25.471 / 分子数: 1 / 由来: Danio rerio / 参照: UniProt: Q1LWJ6
配列:

GSRIYDNTAL LNKPVHNEKL SFTWDPIVHQ SGHGVILEGT VVDFSRHAIT DVKNRKERYN VLYIKPSRVH RRKYDSKGNE IEPNFSDTKK VNTGFLMSSF KVEAKGETDC LDERQLREIV NKEQLVKVTI KHCPREAFAF WISEAEMDKT ELEPGQEVRL KTKGDGPFIF SSAKLDSGTV TKCNFAGDEN AGASWTEKIM ANKSNQENTG KSAAQGEGAD DDEWDD

実験情報

• ビーム: 設備名称: SOLEIL SWING / 地域: Saint-Aubin / 国: France / 線源: X-ray synchrotron / 波長: 0.1 Å / スペクトロメータ・検出器間距離: 1.82 mm
• 検出器: 名称: AVIEX PCCD170170 / タイプ: CCD

スキャン

タイトル: Zebrafish Arpin / 測定日: 2014年12月4日 / 保管温度: 15 °C / セル温度: 15 °C / 照射時間: 1.5 sec. / 単位: 1/nm /

	Min	Max
Q	0.062	6.0833

距離分布関数 P(R)

ソフトウェア P(R): GNOM 5.0 / ポイント数: 591 /

	Min	Max
Q	0.112661	3.43208
P(R) point	1	591
R	0	13.8

結果

カーブのタイプ: single_conc
コメント: Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

	実験値	Porod
分子量	30.8 kDa	-
体積	-	46.6 nm3

	P(R)	Guinier	P(R) error
前方散乱 I0	0.04	0.041	-
慣性半径, Rg	2.79 nm	2.69 nm	0.04

	Min	Max
D	-	13.8
Guinier point	9	83