SASDBU2: Human Arpin (isoform 1) (Human Arpin)

Basic information

Entry

Database: SASBDB / ID: SASDBU2

Sample

Human Arpin (isoform 1)

• Human Arpin (protein), Human Arpin, Homo sapiens

• Function / homology: Arpin / Arp2/3-interacting proteins Arpin / negative regulation of lamellipodium morphogenesis / negative regulation of actin nucleation / directional locomotion / negative regulation of cell migration / lamellipodium / Arpin
• Biological species: Homo sapiens (human)
• Citation: Journal: Structure / Year: 2016; Title: Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.; Authors: Susan Fetics / Aurélien Thureau / Valérie Campanacci / Magali Aumont-Nicaise / Irène Dang / Alexis Gautreau / Javier Pérez / Jacqueline Cherfils / ; Abstract: Arpin is a newly discovered regulator of actin polymerization at the cell leading edge, which steers cell migration by exerting a negative control on the Arp2/3 complex. Arpin proteins have an acidic tail homologous to the acidic motif of the VCA domain of nucleation-promoting factors (NPFs). This tail is predicted to compete with the VCA of NPFs for binding to the Arp2/3 complex, thereby mitigating activation and/or tethering of the complex to sites of actin branching. Here, we investigated the structure of full-length Arpin using synchrotron small-angle X-ray scattering, and of its acidic tail in complex with an ankyrin repeats domain using X-ray crystallography. The data were combined in a hybrid model in which the acidic tail extends from the globular core as a linear peptide and forms a primary epitope that is readily accessible in unbound Arpin and suffices to tether Arpin to interacting proteins with high affinity.

Contact author

• Aurélien Thureau (Soleil, Soleil Synchrotron, Saint-Aubin, France)

Models

Sample

• Sample: Name: Human Arpin (isoform 1)
• Buffer: Name: 50 mM HEPES 100mM NaCl 1mM TCEP / Concentration: 50.00 mM / pH: 7.5 / Composition: 100mM NaCl, 1mM TCEP

Entity #275

Name: Human Arpin / Type: protein / Description: Human Arpin / Formula weight: 24.869 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q7Z6K5
Sequence:

GSRIYHDGAL RNKAVQSVRL PGAWDPAAHQ GGNGVLLEGE LIDVSRHSIL DTHGRKERYY VLYIRPSHIH RRKFDAKGNE IEPNFSATRK VNTGFLMSSY KVEAKGDTDR LTPEALKGLV NKPELLALTE SLTPDHTVAF WMPESEMEVM ELELGAGVRL KTRGDGPFLD SLAKLEAGTV TKCNFTGDGK TGASWTDNIM AQKCSKGAAA EIREQGDGAE DEEWDD

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm
• Detector: Name: AVIEX PCCD170170 / Type: CCD

Scan

Title: Human Arpin (isoform 1) / Measurement date: Dec 4, 2014 / Storage temperature: 15 °C / Cell temperature: 15 °C / Exposure time: 1.5 sec. / Unit: 1/nm /

	Min	Max
Q	0.062	6.0833

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 791 /

	Min	Max
Q	0.112661	4.5532
P(R) point	1	791
R	0	13.2

Result

Type of curve: single_conc
Comments: Due to high concentration at the elution peak, the HPLC UV detector was saturated and the concentrations of the sample was consequently underestimated. In this case, the molecular mass is overestimated when assessing this parameter from I(0) and concentration.

	Experimental	Standard	Porod
MW	27.4 kDa	27.4 kDa	27.8 kDa
Volume	-	-	47.23 nm3

	P(R)	Guinier	P(R) error
Forward scattering, I0	0.05	0.05	-
Radius of gyration, Rg	2.77 nm	2.6 nm	0.05

	Min	Max
D	-	13.2
Guinier point	10	82