SASDBT2: Ankyrin repeat domains from human Tankyrase-2 (489-649)

Basic information

Entry

Database: SASBDB / ID: SASDBT2

Sample

Ankyrin repeat domains from human Tankyrase-2 (489-649)

• Ankyrin repeat domains from Tankyrase 2 (protein), Tankyrase-2(489-649), Homo sapiens

Function / homology

Function:

XAV939 stabilizes AXIN / NAD+ ADP-ribosyltransferase / protein localization to chromosome, telomeric region / negative regulation of telomere maintenance via telomere lengthening / protein auto-ADP-ribosylation / protein poly-ADP-ribosylation / pericentriolar material / positive regulation of telomere capping / NAD+-protein ADP-ribosyltransferase activity / NAD+ ADP-ribosyltransferase activity / Transferases; Glycosyltransferases; Pentosyltransferases / positive regulation of telomere maintenance via telomerase / nucleotidyltransferase activity / TCF dependent signaling in response to WNT / Degradation of AXIN / Wnt signaling pathway / Regulation of PTEN stability and activity / protein polyubiquitination / positive regulation of canonical Wnt signaling pathway / nuclear envelope / chromosome, telomeric region / Ub-specific processing proteases / Golgi membrane / perinuclear region of cytoplasm / enzyme binding / metal ion binding / nucleus / cytosol / cytoplasm

Domain/homology:

Ankyrin repeat / Ankyrin repeats (many copies) / Poly(ADP-ribose) polymerase catalytic domain / Poly(ADP-ribose) polymerase, catalytic domain / PARP catalytic domain profile. / SAM domain (Sterile alpha motif) / SAM domain profile. / Sterile alpha motif. / Sterile alpha motif domain / Sterile alpha motif/pointed domain superfamily / Ankyrin repeat / Ankyrin repeats (3 copies) / Ankyrin repeat profile. / Ankyrin repeat region circular profile. / ankyrin repeats / Ankyrin repeat / Ankyrin repeat-containing domain superfamily

Component:

Poly [ADP-ribose] polymerase tankyrase-2

• Biological species: Homo sapiens (human)
• Citation: Journal: Structure / Year: 2016; Title: Hybrid Structural Analysis of the Arp2/3 Regulator Arpin Identifies Its Acidic Tail as a Primary Binding Epitope.; Authors: Susan Fetics / Aurélien Thureau / Valérie Campanacci / Magali Aumont-Nicaise / Irène Dang / Alexis Gautreau / Javier Pérez / Jacqueline Cherfils / ; Abstract: Arpin is a newly discovered regulator of actin polymerization at the cell leading edge, which steers cell migration by exerting a negative control on the Arp2/3 complex. Arpin proteins have an acidic tail homologous to the acidic motif of the VCA domain of nucleation-promoting factors (NPFs). This tail is predicted to compete with the VCA of NPFs for binding to the Arp2/3 complex, thereby mitigating activation and/or tethering of the complex to sites of actin branching. Here, we investigated the structure of full-length Arpin using synchrotron small-angle X-ray scattering, and of its acidic tail in complex with an ankyrin repeats domain using X-ray crystallography. The data were combined in a hybrid model in which the acidic tail extends from the globular core as a linear peptide and forms a primary epitope that is readily accessible in unbound Arpin and suffices to tether Arpin to interacting proteins with high affinity.

Contact author

• Aurélien Thureau (Soleil, Soleil Synchrotron, Saint-Aubin, France)

Models

Sample

• Sample: Name: Ankyrin repeat domains from human Tankyrase-2 (489-649)
• Buffer: Name: 50 mM HEPES 100mM NaCl 1mM TCEP / Concentration: 50.00 mM / pH: 7.5 / Composition: 100mM NaCl, 1mM TCEP

Entity #274

Name: Tankyrase-2(489-649) / Type: protein / Description: Ankyrin repeat domains from Tankyrase 2 / Formula weight: 17.565 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: Q9H2K2
Sequence:

GNSEADRQLL EAAKAGDVET VKKLCTVQSV NCRDIEGRQS TPLHFAAGYN RVSVVEYLLQ HGADVHAKDK GGLVPLHNAC SYGHYEVAEL LVKHGAVVNV ADLWKFTPLH EAAAKGKYEI CKLLLQHGAD PTKKNRDGNT PLDLVKDGDT DIQDLLRGDA AL

Experimental information

• Beam: Instrument name: SOLEIL SWING / City: Saint-Aubin / 国: France / Type of source: X-ray synchrotronSynchrotron / Wavelength: 0.1 Å / Dist. spec. to detc.: 1.82 mm
• Detector: Name: AVIEX PCCD170170 / Type: CCD

Scan

Title: Ankyrin repeat domains from human Tankyrase-2 / Measurement date: Dec 4, 2014 / Storage temperature: 15 °C / Exposure time: 1.5 sec. / Unit: 1/nm /

	Min	Max
Q	0.062	6.0833

Distance distribution function P(R)

Sofotware P(R): GNOM 5.0 / Number of points: 928 /

	Min	Max
Q	0.185891	5.3918
P(R) point	1	928
R	0	6.25

Result

Type of curve: single_conc

	Experimental	Standard	Porod
MW	17.1 kDa	17.1 kDa	15 kDa
Volume	-	-	23.6 nm3

	P(R)	P(R) error	Guinier	Guinier error
Forward scattering, I0	0.0444	2.0E-5	0.044	3.0E-5
Radius of gyration, Rg	1.84 nm	0.001	1.77 nm	0.01

	Min	Max
D	-	6.25
Guinier point	23	111