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- SASDBJ2: Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDN... -

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Basic information

Entry
Database: SASBDB / ID: SASDBJ2
SampleWild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.
  • 40bp long dsDNA-Sa Oligonucleotide (DNA), dsDNA-Sa Oligo
  • Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family). (protein), ABfR1-WT, Sulfolobus acidocaldarius
Function / homologyTranscription regulator TrmB, N-terminal / Sugar-specific transcriptional regulator TrmB / Winged helix DNA-binding domain superfamily / Winged helix-like DNA-binding domain superfamily / Transcriptional regulator ArsR family
Function and homology information
Biological speciesSulfolobus acidocaldarius (acidophilic)
Contact author
  • Ankan Banerjee (Philipps-Universität Marburg, Marburg, Germany)

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Models

Model #400
Type: dummy / Radius of dummy atoms: 4.20 A / Chi-square value: 1.547 / P-value: 0.000002
Search similar-shape structures of this assembly by Omokage search (details)

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Sample

SampleName: Wild-type archaeal biofilm regulator 1 (ABfR1) bound to 40bp dsDNA-Sa oligonucleotide.
Specimen concentration: 3.5 mg/ml / Entity id: 262 / 263
BufferName: 0.5 x Tris/Borate/EDTA (TBE) / Concentration: 0.50 v/v
Entity #262Name: dsDNA-Sa Oligo / Type: DNA / Description: 40bp long dsDNA-Sa Oligonucleotide / Formula weight: 24.7 / Num. of mol.: 1
Sequence:
GTAGTAAGAT TTAAAAAAAC ATACTAAGAT ATAAATTAAC
Entity #263Name: ABfR1-WT / Type: protein
Description: Wild-type archaeal biofilm regulator 1 (ABfR1: Transcriptional regulator ArsR family).
Formula weight: 13.16 / Num. of mol.: 2 / Source: Sulfolobus acidocaldarius / References: UniProt: Q4JBH1
Sequence:
MSIEITEKYV ILKRFLSVAY GLSESEIDTF LRILESKEGK DIDAISAELK ISKSRTSIIL KKLSDAGLVD KEKGDNMKGG RPKYVYSVNK EELKNRLIAK SEELCKELNQ VIKSFI

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Experimental information

BeamInstrument name: ESRF BM29 / City: Grenoble / 国: France / Type of source: X-ray synchrotron / Wavelength: 0.93 Å / Dist. spec. to detc.: 2.43 mm
DetectorName: Pilatus 1M
Scan
Title: WT archaeal biofilm regulator 1 (ABfR1) + DNA / Measurement date: Aug 27, 2015 / Storage temperature: 4 °C / Cell temperature: 4 °C / Exposure time: 20 sec. / Number of frames: 10 / Unit: 1/nm /
MinMax
Q0.0327 4.9336
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 455 /
MinMax
Q0.291372 2.4267
P(R) point1 455
R0 11.76
Result
Type of curve: single_conc /
ExperimentalStandardPorod
MW51.3 kDa47.93 kDa41 kDa

P(R)Guinier
Forward scattering, I051.3 47.93
Radius of gyration, Rg3.35 nm3 nm

MinMax
D-11.76
Guinier point52 83

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