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データを開く
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基本情報
登録情報 | データベース: SASBDB / ID: SASDBG8 |
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![]() | Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I)
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機能・相同性 | ![]() regulation of type III interferon production / RIG-I signaling pathway / positive regulation of myeloid dendritic cell cytokine production / OAS antiviral response / detection of virus / NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10 / positive regulation of response to cytokine stimulus / positive regulation of granulocyte macrophage colony-stimulating factor production / RSV-host interactions / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() 類似検索 - 分子機能 |
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![]() | ![]() タイトル: Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis. 著者: Neelam Shah / Simone A Beckham / Jacqueline A Wilce / Matthew C J Wilce / ![]() 要旨: RIG-I (retinoic acid inducible gene-I) is a cytosolic innate immune protein that senses viral dsRNA with a 5'-triphosphate overhang. Upon interaction with dsRNA a de-repression of the RIG-I CARD ...RIG-I (retinoic acid inducible gene-I) is a cytosolic innate immune protein that senses viral dsRNA with a 5'-triphosphate overhang. Upon interaction with dsRNA a de-repression of the RIG-I CARD domains takes place that ultimately leads to the production of type I interferons and pro-inflammatory cytokines. Here we investigate the RIG-I conformational rearrangement upon interaction with an activating 5'-triphosphate-10-base pair dsRNA hairpin loop (10bp) compared with a less active 5'-triphosphate-8-base pair dsRNA hairpin loop (8bp). We use size-exclusion chromatography-coupled small-angle X-ray scattering (SAXS) and limited tryptic digest experiments to show that that upon binding to 10 bp, but not 8 bp, RIG-I becomes extended and shows greater flexibility, reflecting the release of its CARDs. We also examined the effect of different ATP analogues on the conformational changes of RIG-I/dsRNA complexes. Of the analogues tested, the addition of ATP transition state analogue ADP-AlFx further assisted in the complete activation of RIG-I in complex with 10bp and also to some extent RIG-I bound to 8bp. Together these data provide solution-based evidence for the molecular mechanism of innate immune signaling by RIG-I as stimulated by short hairpin RNA and ATP. |
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構造の表示
構造ビューア | 分子: ![]() ![]() |
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ダウンロードとリンク
-モデル
モデル #930 | ![]() タイプ: mix / ソフトウェア: (v8.1) / 対称性 ![]() ![]() |
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モデル #935 | ![]() タイプ: dummy / ソフトウェア: (v1.1.2) / ダミー原子の半径: 3.50 A / 対称性 ![]() ![]() |
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試料
![]() | 名称: Probable ATP-dependent RNA helicase DDX58 without CARDs (Delta-CARDs RIG-I) |
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バッファ | 名称: 25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT pH: 7.4 |
要素 #547 | 名称: DC-RIG-I / タイプ: protein 記述: Probable ATP-dependent RNA helicase DDX58 (without CARDs) 分子量: 79.77 / 分子数: 1 / 由来: Homo sapiens / 参照: UniProt: O95786 配列: MHHHHHHAAA SPFKPRNYQL ELALPAMKGK NTIICAPTGC GKTFVSLLIC EHHLKKFPQG QKGKVVFFAN QIPVYEQQKS VFSKYFERHG YRVTGISGAT AENVPVEQIV ENNDIIILTP QILVNNLKKG TIPSLSIFTL MIFDECHNTS KQHPYNMIMF NYLDQKLGGS ...配列: MHHHHHHAAA SPFKPRNYQL ELALPAMKGK NTIICAPTGC GKTFVSLLIC EHHLKKFPQG QKGKVVFFAN QIPVYEQQKS VFSKYFERHG YRVTGISGAT AENVPVEQIV ENNDIIILTP QILVNNLKKG TIPSLSIFTL MIFDECHNTS KQHPYNMIMF NYLDQKLGGS SGPLPQVIGL TASVGVGDAK NTDEALDYIC KLCASLDASV IATVKHNLEE LEQVVYKPQK FFRKVESRIS DKFKYIIAQL MRDTESLAKR ICKDLENLSQ IQNREFGTQK YEQWIVTVQK ACMVFQMPDK DEESRICKAL FLYTSHLRKY NDALIISEHA RMKDALDYLK DFFSNVRAAG FDEIEQDLTQ RFEEKLQELE SVSRDPSNEN PKLEDLCFIL QEEYHLNPET ITILFVKTRA LVDALKNWIE GNPKLSFLKP GILTGRGKTN QNTGMTLPAQ KCILDAFKAS GDHNILIATS VADEGIDIAQ CNLVILYEYV GNVIKMIQTR GRGRARGSKC FLLTSNAGVI EKEQINMYKE KMMNDSILRL QTWDEAVFRE KILHIQTHEK FIRDSQEKPK PVPDKENKKL LCRKCKALAC YTADVRVIEE CHYTVLGDAF KECFVSRPHP KPKQFSSFEK RAKIFCARQN CSHDWGIHVK YKTFEIPVIK IESFVVEDIA TGVQTLYSKW KDFHFEKIPF DPAEMSK |
-実験情報
ビーム | 設備名称: Australian Synchrotron SAXS/WAXS / 地域: Melbourne / 国: Australia ![]() ![]() ![]() | |||||||||||||||||||||||||||
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検出器 | 名称: Pilatus 1M | |||||||||||||||||||||||||||
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距離分布関数 P(R) |
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結果 |
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