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Yorodumi- SASDBC9: GDP bound form of C-terminal deletion mutant of ObgE from E.coli ... -
+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDBC9 |
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Sample | GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP)
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Function / homology | Function and homology information guanyl ribonucleotide binding / dormancy process / negative regulation of ribosome biogenesis / guanosine tetraphosphate binding / ribosomal large subunit binding / ribosome assembly / chromosome segregation / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / GDP binding / rRNA binding ...guanyl ribonucleotide binding / dormancy process / negative regulation of ribosome biogenesis / guanosine tetraphosphate binding / ribosomal large subunit binding / ribosome assembly / chromosome segregation / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / GDP binding / rRNA binding / GTPase activity / GTP binding / magnesium ion binding / DNA binding / cytosol Similarity search - Function |
Biological species | Escherichia coli (strain K12) (bacteria) |
Citation | Date: 2017 Feb 21 Title: Structural and Biochemical Analysis of Escherichia coli ObgE, a Central Regulator of Bacterial Persistence Authors: Gkekas S / Singh R / Shkumatov A / Messens J / Fauvart M / Verstraeten N / Michiels J |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #1118 | Type: dummy / Software: DAMMIN (v2.7.2) / Radius of dummy atoms: 2.50 A / Symmetry: P1 Comment: Average model generated by DAMAVER and used as a starting model for refinement with DAMMIN (r7897M) Chi-square value: 0.606 / P-value: 0.531000 Search similar-shape structures of this assembly by Omokage search (details) |
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-Sample
Sample | Name: GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP) Specimen concentration: 1.00-8.00 |
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Buffer | Name: 20 mM Hepes, 300 mM NaCl, 250 mM imidazole, 5 mM MgCl2, 2 mM DTT, 400 uM GDP pH: 7.5 |
Entity #421 | Name: ObgE_340 / Type: protein / Description: GTPase ObgE/CgtA / Formula weight: 38.899 / Num. of mol.: 1 / Source: Escherichia coli (strain K12) / References: UniProt: P42641 Sequence: MGSSHHHHHH SSGLVPRGSH MKFVDEASIL VVAGDGGNGC VSFRREKYIP KGGPDGGDGG DGGDVWMEAD ENLNTLIDYR FEKSFRAERG QNGASRDCTG KRGKDVTIKV PVGTRVIDQG TGETMGDMTK HGQRLLVAKG GWHGLGNTRF KSSVNRTPRQ KTNGTPGDKR ...Sequence: MGSSHHHHHH SSGLVPRGSH MKFVDEASIL VVAGDGGNGC VSFRREKYIP KGGPDGGDGG DGGDVWMEAD ENLNTLIDYR FEKSFRAERG QNGASRDCTG KRGKDVTIKV PVGTRVIDQG TGETMGDMTK HGQRLLVAKG GWHGLGNTRF KSSVNRTPRQ KTNGTPGDKR ELLLELMLLA DVGMLGMPNA GKSTFIRAVS AAKPKVADYP FTTLVPSLGV VRMDNEKSFV VADIPGLIEG AAEGAGLGIR FLKHLERCRV LLHLIDIDPI DGTDPVENAR IIISELELYS QDLATKPRWL VFNKIDLLDK VEAEEKAKAI AEALGWEDKY YLISAASGLG VKDLCWDVMT FIIENPVA |
-Experimental information
Beam | Instrument name: Rigaku BioSAXS-2000 / City: Brussels / 国: Belgium / Type of source: X-ray in house / Wavelength: 1.54 Å / Dist. spec. to detc.: 1 mm | ||||||||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 100K / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||||||||
Scan | Title: GDP bound form of C-terminal deletion mutant of ObgE from E.coli (ObgE_340 with GDP) Measurement date: Feb 1, 2015 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 1800 sec. / Number of frames: 3 / Unit: 1/A /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.6 / Number of points: 461 /
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Result | Type of curve: merged /
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