- SASDBC7: Human NEI like DNA glycosylase 1 (NEIL1) (Endonuclease 8-like 1) -
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Open data
ID or keywords:
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Basic information
Entry
Database: SASBDB / ID: SASDBC7
Sample
Human NEI like DNA glycosylase 1 (NEIL1)
Endonuclease 8-like 1 (protein), Homo sapiens
Function / homology
Function and homology information
negative regulation of nuclease activity / Defective Base Excision Repair Associated with NEIL1 / depyrimidination / DNA N-glycosylase activity / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / hydrolase activity, acting on glycosyl bonds / Hydrolases; Glycosylases; Hydrolysing N-glycosyl compounds / APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway / DNA-(apurinic or apyrimidinic site) endonuclease activity / Recognition and association of DNA glycosylase with site containing an affected pyrimidine ...negative regulation of nuclease activity / Defective Base Excision Repair Associated with NEIL1 / depyrimidination / DNA N-glycosylase activity / 8-oxo-7,8-dihydroguanine DNA N-glycosylase activity / hydrolase activity, acting on glycosyl bonds / Hydrolases; Glycosylases; Hydrolysing N-glycosyl compounds / APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway / DNA-(apurinic or apyrimidinic site) endonuclease activity / Recognition and association of DNA glycosylase with site containing an affected pyrimidine / Cleavage of the damaged pyrimidine / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / base-excision repair, gap-filling / base-excision repair / chromosome / response to oxidative stress / damaged DNA binding / centrosome / zinc ion binding / nucleoplasm / nucleus / cytoplasm / cytosol Similarity search - Function
Journal: Nucleic Acids Res / Year: 2017 Title: Destabilization of the PCNA trimer mediated by its interaction with the NEIL1 DNA glycosylase. Authors: Aishwarya Prakash / Kedar Moharana / Susan S Wallace / Sylvie Doublié / Abstract: The base excision repair (BER) pathway repairs oxidized lesions in the DNA that result from reactive oxygen species generated in cells. If left unrepaired, these damaged DNA bases can disrupt ...The base excision repair (BER) pathway repairs oxidized lesions in the DNA that result from reactive oxygen species generated in cells. If left unrepaired, these damaged DNA bases can disrupt cellular processes such as replication. NEIL1 is one of the 11 human DNA glycosylases that catalyze the first step of the BER pathway, i.e. recognition and excision of DNA lesions. NEIL1 interacts with essential replication proteins such as the ring-shaped homotrimeric proliferating cellular nuclear antigen (PCNA). We isolated a complex formed between NEIL1 and PCNA (±DNA) using size exclusion chromatography (SEC). This interaction was confirmed using native gel electrophoresis and mass spectrometry. Stokes radii measured by SEC hinted that PCNA in complex with NEIL1 (±DNA) was no longer a trimer. Height measurements and images obtained by atomic force microscopy also demonstrated the dissociation of the PCNA homotrimer in the presence of NEIL1 and DNA, while small-angle X-ray scattering analysis confirmed the NEIL1 mediated PCNA trimer dissociation and formation of a 1:1:1 NEIL1-DNA-PCNA(monomer) complex. Furthermore, ab initio shape reconstruction provides insights into the solution structure of this previously unreported complex. Together, these data point to a potential mechanistic switch between replication and BER.
Contact author
Kedar Moharana (University of Vermont, Burlington, VT, USA)
Instrument name: Advanced Photon Source (APS) BioCAT 18ID / City: Argonne, IL / 国: United States / Type of source: X-ray synchrotron / Wavelength: 0.1033 Å
Detector
Name: MAR 165 CCD
Scan
Title: Human NEI like DNA glycosylase 1 (NEIL1) / Measurement date: Mar 13, 2015 / Storage temperature: 4 °C / Cell temperature: 10 °C / Exposure time: 1 sec. / Number of frames: 14 / Unit: 1/A /
Min
Max
Q
0.005
0.3286
Distance distribution function P(R)
Sofotware P(R): GNOM 5.0 / Number of points: 1062 /
Min
Max
Q
0.0130078
0.328583
P(R) point
1
1062
R
0
150
Result
Type of curve: single_conc Comments: Experimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of ...Comments: Experimental MW reported here is calculated from Gnom based I(0) estimates and will be used for publication purpose in future. Guinier I(0) estimates are prone to presence of aggregates/radiation damage and that is why Gnom based I(0) was preferred to calculate the experimental MW.
Experimental
Standard
Standard error
Porod
MW
48.1 kDa
50.56 kDa
0.35
47.8 kDa
Volume
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-
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81.37 nm3
P(R)
P(R) error
Guinier
Guinier error
Forward scattering, I0
4.411
0.0441
4.31
0.03
Radius of gyration, Rg
3.83 nm
0.064
3.55 nm
0.331
Min
Max
Error
D
-
15
0.25
Guinier point
28
107
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