SASDAU3: Fila22-23 (immunoglobulin- like filamin two-domain fragment 22-23)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDAU3

試料

Fila22-23

• immunoglobulin- like filamin two-domain fragment 22-23 (protein), Fila22-23, Escherichia coli

• 生物種: Escherichia coli (大腸菌)
• 引用: ジャーナル: J Biol Chem / 年: 2009; タイトル: Atomic structures of two novel immunoglobulin-like domain pairs in the actin cross-linking protein filamin.; 著者: Outi K Heikkinen / Salla Ruskamo / Peter V Konarev / Dmitri I Svergun / Tatu Iivanainen / Sami M Heikkinen / Perttu Permi / Harri Koskela / Ilkka Kilpeläinen / Jari Ylänne / ; 要旨: Filamins are actin filament cross-linking proteins composed of an N-terminal actin-binding domain and 24 immunoglobulin-like domains (IgFLNs). Filamins interact with numerous proteins, including the cytoplasmic domains of plasma membrane signaling and cell adhesion receptors. Thereby filamins mechanically and functionally link the cell membrane to the cytoskeleton. Most of the interactions have been mapped to the C-terminal IgFLNs 16-24. Similarly, as with the previously known compact domain pair of IgFLNa20-21, the two-domain fragments IgFLNa16-17 and IgFLNa18-19 were more compact in small angle x-ray scattering analysis than would be expected for two independent domains. Solution state NMR structures revealed that the domain packing in IgFLNa18-19 resembles the structure of IgFLNa20-21. In both domain pairs the integrin-binding site is masked, although the details of the domain-domain interaction are partly distinct. The structure of IgFLNa16-17 revealed a new domain packing mode where the adhesion receptor binding site of domain 17 is not masked. Sequence comparison suggests that similar packing of three tandem filamin domain pairs is present throughout the animal kingdom, and we propose that this packing is involved in the regulation of filamin interactions through a mechanosensor mechanism.

登録者

• Petr Konarev (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #39: ; タイプ: dummy / ソフトウェア: Gasbor and Damaver / カイ2乗値: 3.1684; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Fila22-23 / Sample MW: 18.7 kDa
• バッファ: 名称: 100 mM NaCl, 10 mM dithiothreitol, 20 mM Tris / pH: 8

要素 #36

名称: Fila22-23 / タイプ: protein
記述: immunoglobulin- like filamin two-domain fragment 22-23
分子量: 18.7 / 分子数: 1 / 由来: Escherichia coli
配列:

PAADPEKSYA EGPGLDGGEC FQPSKFKIHA VDPDGVDGGD GFVVTIEGPA PVDPVMVDNG DGTYDVEFEP KEAGDYVINL TLDGDNVNGF PKTVTVKPAP SAEHSYAEGE GLVKVFDNAP AEFTIFAVDT KGVARTDGGD PFEVAINGPD GLVVDAKVTD NNDGTYGVVY DAPVEGNYNV NVTLRGNPIK NMPIDVKCIE

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron
• 検出器: 名称: MAR 345 Image Plate

スキャン

タイトル: Fila22-23 / 測定日: 2007年6月18日 / 単位: 1/nm /

	Min	Max
Q	0.235	5.0544

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 474 /

	Min	Max
Q	0.2733	2.464
P(R) point	32	505
R	0	9

結果

D max: 9 / カーブのタイプ: single_conc /

	実験値	Porod	Estimated
分子量	24 kDa	-	-
体積	-	32 nm3	32

	P(R)	Guinier
前方散乱 I0	34	34.3
慣性半径, Rg	2.77 nm	2.76 nm