SASDAN4: Calmodulin:peptide complex (Calmodulin + C-terminal region of hum...

Basic information

• Entry: Database: SASBDB / ID: SASDAN4

Sample

Calmodulin:peptide complex

• Calmodulin (protein), Homo sapiens
• C-terminal region of human myelin basic protein (protein), peptide, Homo sapiens

Function / homology

Function:

: / : / : / : / : / positive regulation of protein autophosphorylation / negative regulation of peptidyl-threonine phosphorylation / establishment of protein localization to mitochondrial membrane / type 3 metabotropic glutamate receptor binding / positive regulation of peptidyl-threonine phosphorylation / positive regulation of DNA binding / response to corticosterone / negative regulation of high voltage-gated calcium channel activity / negative regulation of ryanodine-sensitive calcium-release channel activity / negative regulation of calcium ion export across plasma membrane / regulation of cardiac muscle cell action potential / nitric-oxide synthase binding / regulation of cell communication by electrical coupling involved in cardiac conduction / regulation of synaptic vesicle exocytosis / adenylate cyclase binding / protein phosphatase activator activity / positive regulation of protein serine/threonine kinase activity / catalytic complex / detection of calcium ion / regulation of synaptic vesicle endocytosis / regulation of cardiac muscle contraction / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / activation of adenylate cyclase activity / phosphatidylinositol 3-kinase binding / positive regulation of nitric-oxide synthase activity / regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum / enzyme regulator activity / titin binding / regulation of calcium-mediated signaling / voltage-gated potassium channel complex / calcium channel complex / substantia nigra development / regulation of heart rate / response to amphetamine / adenylate cyclase activator activity / sarcomere / protein serine/threonine kinase activator activity / nitric-oxide synthase regulator activity / regulation of cytokinesis / spindle microtubule / response to calcium ion / mitochondrial membrane / G2/M transition of mitotic cell cycle / spindle pole / disordered domain specific binding / calcium-dependent protein binding / synaptic vesicle membrane / myelin sheath / growth cone / vesicle / transmembrane transporter binding / G protein-coupled receptor signaling pathway / protein domain specific binding / calcium ion binding / centrosome / protein kinase binding / protein-containing complex / nucleus / plasma membrane / cytoplasm

Domain/homology:

EF-hand domain pair / EF-hand, calcium binding motif / EF-Hand 1, calcium-binding site / EF-hand calcium-binding domain. / EF-hand calcium-binding domain profile. / EF-hand domain / EF-hand domain pair

Component:

Calmodulin-3

• Biological species: Homo sapiens (human)
• Citation: Journal: BMC Struct Biol / Year: 2008; Title: Interaction between the C-terminal region of human myelin basic protein and calmodulin: analysis of complex formation and solution structure.; Authors: Viivi Majava / Maxim V Petoukhov / Nobuhiro Hayashi / Päivi Pirilä / Dmitri I Svergun / Petri Kursula / ; Abstract: BACKGROUND: The myelin sheath is a multilamellar membrane structure wrapped around the axon, enabling the saltatory conduction of nerve impulses in vertebrates. Myelin basic protein, one of the most abundant myelin-specific proteins, is an intrinsically disordered protein that has been shown to bind calmodulin. In this study, we focus on a 19-mer synthetic peptide from the predicted calmodulin-binding segment near the C-terminus of human myelin basic protein.; RESULTS: The interaction of native human myelin basic protein with calmodulin was confirmed by affinity chromatography. The binding of the myelin basic protein peptide to calmodulin was tested with isothermal titration calorimetry (ITC) in different temperatures, and Kd was observed to be in the low muM range, as previously observed for full-length myelin basic protein. Surface plasmon resonance showed that the peptide bound to calmodulin, and binding was accompanied by a conformational change; furthermore, gel filtration chromatography indicated a decrease in the hydrodynamic radius of calmodulin in the presence of the peptide. NMR spectroscopy was used to map the binding area to reside mainly within the hydrophobic pocket of the C-terminal lobe of calmodulin. The solution structure obtained by small-angle X-ray scattering indicates binding of the myelin basic protein peptide into the interlobal groove of calmodulin, while calmodulin remains in an extended conformation.; CONCLUSION: Taken together, our results give a detailed structural insight into the interaction of calmodulin with a C-terminal segment of a major myelin protein, the myelin basic protein. The used 19-mer peptide interacts mainly with the C-terminal lobe of calmodulin, and a conformational change accompanies binding, suggesting a novel mode of calmodulin-target protein interaction. Calmodulin does not collapse and wrap around the peptide tightly; instead, it remains in an extended conformation in the solution structure. The observed affinity can be physiologically relevant, given the high abundance of both binding partners in the nervous system.

Contact author

• Maxim Petoukhov (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

Models

• Model #63: ; Type: atomic / Software: Sasref / Chi-square value: 4.7524; Search similar-shape structures of this assembly by Omokage search (details)

Sample

• Sample: Name: Calmodulin:peptide complex / Sample MW: 18.84 kDa / Specimen concentration: 5.30-20.00 / Entity id: 58 / 59
• Buffer: Name: Tris75 / Concentration: 25.00 mM / pH: 7.5 / Composition: NaCl 200.000 mM

Entity #58

Type: protein / Description: Calmodulin / Formula weight: 16.84 / Num. of mol.: 1 / Source: Homo sapiens / References: UniProt: P62158
Sequence:

MADQLTEEQI AEFKEAFSLF DKDGDGTITT KELGTVMRSL GQNPTEAELQ DMINEVDADG NGTIDFPEFL TMMARKMKDT DSEEEIREAF RVFDKDGNGY ISAAELRHVM TNLGEKLTDE EVDEMIREAD IDGDGQVNYE EFVQMMTAK

Entity #59

Name: peptide / Type: protein
Description: C-terminal region of human myelin basic protein
Formula weight: 2 / Num. of mol.: 1 / Source: Homo sapiens
Sequence:

HKGFKGVDAQ GTLSKIFKL

Experimental information

• Beam: Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron
• Detector: Name: MAR 345 Image Plate

Scan

Title: CaM:Peptide / Measurement date: Nov 28, 2006 / Unit: 1/nm /

	Min	Max
Q	0.3198	5.0097

Distance distribution function P(R)

Sofotware P(R): GNOM 4.5a / Number of points: 512 /

	Min	Max
Q	0.3233	4.995
P(R) point	1	512
R	0	7

Result

I0 from Guinier: 107.587 / Rg from Guinier: 2.1 nm / Type of curve: merged /

	Experimental	Porod
MW	20 kDa	-
Volume	-	36 nm3

/

	Min	Max
D	-	7
Guinier point	1	85