+Open data
-Basic information
Entry | Database: SASBDB / ID: SASDAC4 |
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Sample | FilaminC 23-24
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Biological species | Escherichia coli (E. coli) |
Citation | Journal: J Mol Biol / Year: 2007 Title: Crystal structure of human filamin C domain 23 and small angle scattering model for filamin C 23-24 dimer. Authors: Ljiljana Sjekloća / Regina Pudas / Björn Sjöblom / Peter Konarev / Oliviero Carugo / Vladimir Rybin / Tiila-Riikka Kiema / Dmitri Svergun / Jari Ylänne / Kristina Djinović Carugo / Abstract: Filamin C is a dimeric, actin-binding protein involved in organization of cortical cytoskeleton and of the sarcomere. We performed crystallographic, small-angle X-ray scattering and analytical ...Filamin C is a dimeric, actin-binding protein involved in organization of cortical cytoskeleton and of the sarcomere. We performed crystallographic, small-angle X-ray scattering and analytical ultracentrifugation experiments on the constructs containing carboxy-terminal domains of the protein (domains 23-24 and 19-21). The crystal structure of domain 23 of filamin C showed that the protein adopts the expected immunoglobulin (Ig)-like fold. Small-angle X-ray scattering experiments performed on filamin C tandem Ig-like domains 23 and 24 reveal a dimer that is formed by domain 24 and that domain 23 has little interactions with itself or with domain 24, while the analytical ultracentrifugation experiments showed that the filamin C domains 19-21 form elongated monomers in diluted solutions. |
Contact author |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Models
Model #55 | Type: dummy / Software: Dammin / Symmetry: P2 / Chi-square value: 9.972964 Search similar-shape structures of this assembly by Omokage search (details) |
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Model #56 | Type: mix / Software: Bunch / Radius of dummy atoms: 1.90 A / Symmetry: P2 / Chi-square value: 19.7136 Search similar-shape structures of this assembly by Omokage search (details) |
-Sample
Sample | Name: FilaminC 23-24 / Sample MW: 40 kDa / Specimen concentration: 5.00-21.00 |
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Buffer | Name: 20 mM Tris-HCl / pH: 8 / Composition: NaCl 50.000 mM |
Entity #52 | Name: FilaminC 23-24 / Type: protein / Description: Filamin C 23-24 / Formula weight: 20 / Num. of mol.: 2 / Source: Escherichia coli Sequence: AGDPGLVSAY GPGLEGGTTG VSSEFIVNTL NAGSGALSVT IDGPSKVQLD CRECPEGHVV TYTPMAPGNY LIAIKYGGPQ HIVGSPFKAK VTGPRLSGGH SLHETSTVLV ETVTKSSSSR GSSYSSIPKF SAMGSDASKV VTRGPGLSQA FVGQKNSFTV DCSKAGTNMM ...Sequence: AGDPGLVSAY GPGLEGGTTG VSSEFIVNTL NAGSGALSVT IDGPSKVQLD CRECPEGHVV TYTPMAPGNY LIAIKYGGPQ HIVGSPFKAK VTGPRLSGGH SLHETSTVLV ETVTKSSSSR GSSYSSIPKF SAMGSDASKV VTRGPGLSQA FVGQKNSFTV DCSKAGTNMM MVGVHGPKTP CEEVYVKHMG NRVYNVTYTV KEKGDYILIV KWGDESVPGS PFKVKVP |
-Experimental information
Beam | Instrument name: DORIS III X33 / City: Hamburg / 国: Germany / Type of source: X-ray synchrotron | |||||||||||||||||||||||||||
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Detector | Name: MAR 345 Image Plate | |||||||||||||||||||||||||||
Scan | Title: Filamin C 23-24 / Measurement date: Jun 17, 2005 / Storage temperature: 10 °C / Cell temperature: 10 °C / Exposure time: 120 sec. / Number of frames: 2 / Unit: 1/nm /
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Distance distribution function P(R) | Sofotware P(R): GNOM 4.5a / Number of points: 480 /
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Result | Type of curve: merged /
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