SASDA94: Der p21 (Der p21, allegren, Derp21)

基本情報

• 登録情報: データベース: SASBDB / ID: SASDA94

試料

Der p21

• Der p21, allegren (protein), Derp21, Escherichia coli

• 生物種: Escherichia coli (大腸菌)
• 引用: ジャーナル: Allergy / 年: 2008; タイトル: Characterization of Der p 21, a new important allergen derived from the gut of house dust mites.; 著者: M Weghofer / Y Dall'Antonia / M Grote / A Stöcklinger / M Kneidinger / N Balic / M-T Krauth / E Fernández-Caldas / W R Thomas / M van Hage / S Vieths / S Spitzauer / F Horak / D I Svergun / P V Konarev / P Valent / J Thalhamer / W Keller / R Valenta / S Vrtala / ; 要旨: BACKGROUND: The house dust mite (HDM) Dermatophagoides pteronyssinus is a major allergen source eliciting allergic asthma. The aim of the study was to identify new important HDM allergens associated with allergic asthma.; 手法: A cDNA coding for a new mite allergen, designated Der p 21, was isolated using immunoglobulin E (IgE) antibodies from patients with allergic asthma out of a D. pteronyssinus expression cDNA library and expressed in Escherichia coli.; RESULTS: Circular dichroism analysis of the purified allergen showed that rDer p 21 (14 726 Da) is one of the few mite allergens with an alpha-helical secondary structure. The protein exhibited high thermal stability and refolding capacity, and, as determined by small angle X-ray scattering, formed a dimer consisting of two flat triangles. rDer p 21 bound high levels of patients' IgE antibodies and showed high allergenic activity in basophil activation experiments. Rabbit anti-Der p 21 IgG antibodies inhibited mite-allergic patients' IgE binding and allowed the ultrastructural localization of the allergen in the midgut (epithelium, lumen and faeces) of D. pteronyssinus by immunogold electron microscopy. Der p 21 revealed sequence homology with group 5 mite allergens, but IgE and IgG reactivity data and cross-inhibition studies identified it as a new mite allergen.; CONCLUSIONS: Der p 21 is a new important mite allergen which is liberated into the environment via faecal particles and hence may be associated with allergic asthma.

登録者

• Petr Konarev (EMBL-Hamburg, European Molecular Biology Laboratory (EMBL) - Hamburg outstation, Notkestraße 85, Geb. 25A, 22607 Hamburg, Deutschland, Germany)

モデル

• モデル #49: ; タイプ: dummy / ソフトウェア: dammin / 対称性: P2 / カイ2乗値: 3.493161; Omokage検索でこの集合体の類似形状データを探す (詳細)
• モデル #50: ; タイプ: mix / ソフトウェア: Bunch / ダミー原子の半径: 1.90 A / 対称性: P2 / カイ2乗値: 3.0625; Omokage検索でこの集合体の類似形状データを探す (詳細)

試料

• 試料: 名称: Der p21 / Sample MW: 26 kDa / 試料濃度: 4.7 mg/ml
• バッファ: 名称: 10 mM Hepes / pH: 7

要素 #49

名称: Derp21 / タイプ: protein / 記述: Der p21, allegren / 分子量: 13 / 分子数: 2 / 由来: Escherichia coli
配列:

MFIVGDKKED EWRMAFDRLM MEELETKIDQ VEKGLLHLSE QYKELEKTKS KELKEQILRE LTIGENFMKG ALKFFEMEAK RTDLNMFERY NYEFALESIK LLIKKLDELA KKVKAVNPDE YY

実験情報

• ビーム: 設備名称: DORIS III X33 / 地域: Hamburg / 国: Germany / 線源: X-ray synchrotron
• 検出器: 名称: MAR 345 Image Plate

スキャン

タイトル: der p21 / 測定日: 2006年7月24日 / 保管温度: 10 °C / セル温度: 10 °C / 照射時間: 120 sec. / フレーム数: 2 / 単位: 1/nm /

	Min	Max
Q	0.2005	4.9899

距離分布関数 P(R)

ソフトウェア P(R): GNOM 4.5a / ポイント数: 512 /

	Min	Max
Q	0.2039	4.866
P(R) point	46	557
R	0	8.5

結果

カーブのタイプ: single_conc /

	実験値	Porod
分子量	26 kDa	-
体積	-	50 nm3

	P(R)	Guinier
前方散乱 I0	134	98.1
慣性半径, Rg	2.75 nm	2.65 nm

	Min	Max
D	-	8.5
Guinier point	1	108