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- PDB-9zrg: Structure of naked mole-rat ribosome (non-rotated) -

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Basic information

Entry
Database: PDB / ID: 9zrg
TitleStructure of naked mole-rat ribosome (non-rotated)
Components
  • (40S ribosomal protein ...) x 16
  • (60S ribosomal protein ...) x 27
  • (Large ribosomal subunit protein ...) x 13
  • (Ribosomal protein ...) x 2
  • (Small ribosomal subunit protein ...) x 15
  • (Ubiquitin-ribosomal protein ...) x 2
  • 18S ribosomal RNA
  • 28S ribosomal RNA
  • 5.8S ribosomal RNA
  • 5S ribosomal RNA
  • E-tRNA
  • Ubiquitin-like protein FUBI
KeywordsRIBOSOME / Naked mole rat ribosome
Function / homology
Function and homology information


regulation of G1 to G0 transition / protein-DNA complex disassembly / G1 to G0 transition / neural crest cell differentiation / laminin receptor activity / cellular response to actinomycin D / TOR signaling / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of ubiquitin-dependent protein catabolic process / ubiquitin ligase inhibitor activity ...regulation of G1 to G0 transition / protein-DNA complex disassembly / G1 to G0 transition / neural crest cell differentiation / laminin receptor activity / cellular response to actinomycin D / TOR signaling / positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator / negative regulation of ubiquitin-dependent protein catabolic process / ubiquitin ligase inhibitor activity / 90S preribosome / positive regulation of signal transduction by p53 class mediator / protein-RNA complex assembly / positive regulation of cell cycle / laminin binding / rough endoplasmic reticulum / translation regulator activity / gastrulation / cytosolic ribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / innate immune response in mucosa / mRNA 3'-UTR binding / neural tube closure / small-subunit processome / mRNA 5'-UTR binding / transcription coactivator binding / response to virus / cytoplasmic ribonucleoprotein granule / rRNA processing / kinase activity / antimicrobial humoral immune response mediated by antimicrobial peptide / large ribosomal subunit / antibacterial humoral response / ribosome binding / cell body / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / cell differentiation / rRNA binding / protein stabilization / defense response to Gram-positive bacterium / structural constituent of ribosome / positive regulation of apoptotic process / ribosome / translation / ribonucleoprotein complex / mRNA binding / positive regulation of cell population proliferation / ubiquitin protein ligase binding / synapse / dendrite / positive regulation of gene expression / centrosome / protein kinase binding / negative regulation of apoptotic process / nucleolus / negative regulation of transcription by RNA polymerase II / endoplasmic reticulum / extracellular space / RNA binding / zinc ion binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Trappin protein transglutaminase-binding repeat / Trappin protein transglutaminase binding domain / : / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / metallochaperone-like domain ...Trappin protein transglutaminase-binding repeat / Trappin protein transglutaminase binding domain / : / 40S ribosomal protein SA / 40S ribosomal protein SA, C-terminal domain / 40S ribosomal protein SA C-terminus / Ubiquitin-like protein FUBI / Ribosomal protein L23 / Ribosomal protein L2, archaeal-type / metallochaperone-like domain / TRASH domain / Ribosomal protein L41 / Ribosomal protein L41 / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein L29e / Ribosomal L29e protein family / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / Ribosomal protein S2, eukaryotic / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / S27a-like superfamily / Ribosomal protein L44e signature. / 40S Ribosomal protein S10 / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein S30 / Ribosomal protein S30 / : / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / : / Ribosomal L40e family / Ribosomal protein S7e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein L36e signature. / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein L44e / Ribosomal protein L44 / Ribosomal_L40e / Ribosomal protein L40e / Ribosomal protein L40e superfamily / Ribosomal protein S17e, conserved site / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein S17e signature. / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein S3Ae, conserved site / Ribosomal protein S3Ae signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / : / Ribosomal protein L34Ae / Ribosomal protein L34e / Ribosomal protein S27e signature. / Ribosomal protein S6, eukaryotic / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein S19e / Ribosomal protein S19e / Ribosomal_S19e / Ribosomal protein L31e, conserved site / Ribosomal protein L31e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L37ae / Ribosomal L37ae protein family / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein L39e / Ribosomal protein L39e domain superfamily
Similarity search - Domain/homology
: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein eL31 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 ...: / RNA / RNA (> 10) / RNA (> 100) / RNA (> 1000) / Large ribosomal subunit protein eL31 / Small ribosomal subunit protein uS13 / Large ribosomal subunit protein eL38 / Small ribosomal subunit protein eS10 / Small ribosomal subunit protein uS17 / Small ribosomal subunit protein uS12 / 40S ribosomal protein S6 / Small ribosomal subunit protein RACK1 / Large ribosomal subunit protein uL2 / 40S ribosomal protein S25 / 60S ribosomal protein L41 / Small ribosomal subunit protein uS14 / Ribosomal protein L37 / Small ribosomal subunit protein uS2 / Large ribosomal subunit protein eL39 / 40S ribosomal protein S24 / Large ribosomal subunit protein uL5 / Large ribosomal subunit protein eL43 / 40S ribosomal protein S27 / Large ribosomal subunit protein eL34 / Large ribosomal subunit protein eL21 / Large ribosomal subunit protein eL29 / Small ribosomal subunit protein uS8 / Small ribosomal subunit protein eS19 / Small ribosomal subunit protein uS15 / Small ribosomal subunit protein eS17 / Ubiquitin-like protein FUBI / Small ribosomal subunit protein uS7 / Ubiquitin-ribosomal protein eS31 fusion protein / Ribosomal protein L15 / Small ribosomal subunit protein uS9 / Large ribosomal subunit protein uL14 / 60S ribosomal protein L27 / Large ribosomal subunit protein uL23 / 60S ribosomal protein L36a / 60S ribosomal protein L32 / Large ribosomal subunit protein eL24 / 60S ribosomal protein L36 / Small ribosomal subunit protein eS1 / Large ribosomal subunit protein eL22 / Ubiquitin-ribosomal protein eL40 fusion protein / 40S ribosomal protein S7 / 40S ribosomal protein S21 / Small ribosomal subunit protein uS4
Similarity search - Component
Biological speciesHeterocephalus glaber (naked mole-rat)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.1 Å
AuthorsGutierrez-Vargas, C. / De, S. / Maji, S. / Liu, Z. / Nieb, M. / Seluanov, A. / Gorbunova, V. / Frank, J.
Funding support United States, 3items
OrganizationGrant numberCountry
National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS)R35 GM139453 United States
National Institutes of Health/National Institute on Aging (NIH/NIA)P01 AG047200 United States
National Science Foundation (NSF, United States)DGE-1644869 United States
Citation
Journal: Nucleic Acids Res / Year: 2026
Title: Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?
Authors: Cristina Gutierrez-Vargas / Swastik De / Suvrajit Maji / Zheng Liu / Zhonghe Ke / Martina Nieß / Andrei Seluanov / Vera Gorbunova / Joachim Frank /
Abstract: Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, ...Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, display fragmented 28S ribosomal RNA (rRNA), coupled with high translational fidelity and long lifespan. The unusual ribosomal architecture in the NMR and tuco-tuco has been speculated to be linked to high translational fidelity. Here, we show, by single-particle cryo-electron microscopy, that despite the fragmentation of their rRNA, NMR and tuco-tuco ribosomes retain their core functional architecture. Compared to ribosomes of the guinea pig, a phylogenetically related rodent without 28S rRNA fragmentation, ribosomes of NMR and tuco-tuco exhibit poorly resolved density for certain expansion segments. In contrast, the structure of the guinea pig ribosome shows high similarity to the human ribosome. Enhanced translational fidelity in the NMR and tuco-tuco may stem from subtle, allosteric effects in dynamics, linked to rRNA fragmentation.
#1: Journal: Nucleic Acids Res / Year: 2026
Title: Structures of naked mole-rat, tuco-tuco, and guinea pig ribosomes-is rRNA fragmentation linked to translational fidelity?
Authors: Cristina Gutierrez-Vargas / Swastik De / Suvrajit Maji / Zheng Liu / Zhonghe Ke / Martina Nieß / Andrei Seluanov / Vera Gorbunova / Joachim Frank /
Abstract: Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, ...Ribosomes are central to protein synthesis in all organisms. In mammals, the ribosome functional core is highly conserved. Remarkably, two rodent species, the naked mole-rat (NMR) and tuco-tuco, display fragmented 28S ribosomal RNA (rRNA), coupled with high translational fidelity and long lifespan. The unusual ribosomal architecture in the NMR and tuco-tuco has been speculated to be linked to high translational fidelity. Here, we show, by single-particle cryo-electron microscopy, that despite the fragmentation of their rRNA, NMR and tuco-tuco ribosomes retain their core functional architecture. Compared to ribosomes of the guinea pig, a phylogenetically related rodent without 28S rRNA fragmentation, ribosomes of NMR and tuco-tuco exhibit poorly resolved density for certain expansion segments. In contrast, the structure of the guinea pig ribosome shows high similarity to the human ribosome. Enhanced translational fidelity in the NMR and tuco-tuco may stem from subtle, allosteric effects in dynamics, linked to rRNA fragmentation.
History
DepositionDec 19, 2025Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 18, 2026Provider: repository / Type: Initial release
Revision 1.0Feb 18, 2026Data content type: EM metadata / Data content type: EM metadata / Provider: repository / Type: Initial release
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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
AO: 40S ribosomal protein S14
AX: Small ribosomal subunit protein uS12
AN: Small ribosomal subunit protein uS15
AL: Small ribosomal subunit protein uS17
AB: Small ribosomal subunit protein eS1
AA: Small ribosomal subunit protein uS2
AV: 40S ribosomal protein S21
AY: 40S ribosomal protein S24
Aa: 40S ribosomal protein S26
Ab: 40S ribosomal protein S27
Ae: Ubiquitin-like protein FUBI
AJ: Small ribosomal subunit protein uS4
AE: 40S ribosomal protein S4, X isoform
AC: 40S ribosomal protein S2
AG: 40S ribosomal protein S6
AH: 40S ribosomal protein S7
AW: Small ribosomal subunit protein uS8
AI: 40S ribosomal protein S8
B2: 18S ribosomal RNA
CR: 60S ribosomal protein L19
CW: Large ribosomal subunit protein eL24
Ag: Small ribosomal subunit protein RACK1
AU: 40S ribosomal protein S20
AK: Small ribosomal subunit protein eS10
AM: 40S ribosomal protein S12
AS: Small ribosomal subunit protein uS13
Ad: Small ribosomal subunit protein uS14
AR: Small ribosomal subunit protein eS17
AP: 40S ribosomal protein S15
AT: Small ribosomal subunit protein eS19
AZ: 40S ribosomal protein S25
Ac: 40S ribosomal protein S28
AD: 40S ribosomal protein S3
Af: Ubiquitin-ribosomal protein eS31 fusion protein
AF: Small ribosomal subunit protein uS7
AQ: Small ribosomal subunit protein uS9
Cz: 60S ribosomal protein L10a
CO: 60S ribosomal protein L13a
CL: 60S ribosomal protein L13
CV: Large ribosomal subunit protein uL14
CM: 60S ribosomal protein L14
Ca: 60S ribosomal protein L27a
CN: Ribosomal protein L15
CI: 60S ribosomal protein L10-like
CD: 60S ribosomal protein L5
CQ: 60S ribosomal protein L18
CA: Large ribosomal subunit protein uL2
CS: 60S ribosomal protein L18a
CT: Large ribosomal subunit protein eL21
CP: 60S ribosomal protein L17
CU: Large ribosomal subunit protein eL22
CX: Large ribosomal subunit protein uL23
CY: 60S ribosomal protein L26
CZ: 60S ribosomal protein L27
Cr: 60S ribosomal protein L28
Ch: 60S ribosomal protein L35
Cb: Large ribosomal subunit protein eL29
CB: 60S ribosomal protein L3
CF: 60S ribosomal protein L7
Cc: 60S ribosomal protein L30
Cd: Large ribosomal subunit protein eL31
Ce: 60S ribosomal protein L32
Cf: 60S ribosomal protein L35a
Cg: Large ribosomal subunit protein eL34
Ci: 60S ribosomal protein L36
Cj: Ribosomal protein L37
Ck: Large ribosomal subunit protein eL38
Cl: Large ribosomal subunit protein eL39
CC: 60S ribosomal protein L4
Cm: Ubiquitin-ribosomal protein eL40 fusion protein
Cn: 60S ribosomal protein L41
Cp: Large ribosomal subunit protein eL43
Co: 60S ribosomal protein L36a
CJ: Large ribosomal subunit protein uL5
CH: 60S ribosomal protein L9
CE: 60S ribosomal protein L6
CG: 60S ribosomal protein L7a
A5: 28S ribosomal RNA
A7: 5S ribosomal RNA
A8: 5.8S ribosomal RNA
BC: E-tRNA
A6: 28S ribosomal RNA


Theoretical massNumber of molelcules
Total (without water)4,576,63982
Polymers4,576,63982
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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40S ribosomal protein ... , 16 types, 16 molecules AOAVAYAaAbAEACAGAHAIAUAMAPAZAcAD

#1: Protein 40S ribosomal protein S14


Mass: 14544.659 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#7: Protein 40S ribosomal protein S21


Mass: 8977.214 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps21, GW7_09751 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C8F1
#8: Protein 40S ribosomal protein S24


Mass: 14604.264 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps24 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0AAX6T3G5
#9: Protein 40S ribosomal protein S26


Mass: 12110.308 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#10: Protein 40S ribosomal protein S27


Mass: 9480.186 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps27, GW7_11176 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: E3VX74
#13: Protein 40S ribosomal protein S4, X isoform


Mass: 29654.869 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#14: Protein 40S ribosomal protein S2


Mass: 24961.395 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#15: Protein 40S ribosomal protein S6


Mass: 27471.535 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS6, Rps6 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J125
#16: Protein 40S ribosomal protein S7


Mass: 21716.387 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps7, RPS7, GW7_02294 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C7L2
#18: Protein 40S ribosomal protein S8


Mass: 24003.012 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#23: Protein 40S ribosomal protein S20


Mass: 11765.890 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#25: Protein 40S ribosomal protein S12


Mass: 13776.030 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#29: Protein 40S ribosomal protein S15


Mass: 15178.974 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#31: Protein 40S ribosomal protein S25


Mass: 8526.119 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS25 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J730
#32: Protein 40S ribosomal protein S28


Mass: 7263.394 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#33: Protein 40S ribosomal protein S3


Mass: 25172.561 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)

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Small ribosomal subunit protein ... , 15 types, 15 molecules AXANALABAAAJAWAgAKASAdARATAFAQ

#2: Protein Small ribosomal subunit protein uS12 / 40S ribosomal protein S23


Mass: 15757.587 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS23, Rps23 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J0V6
#3: Protein Small ribosomal subunit protein uS15 / 40S ribosomal protein S13


Mass: 17128.191 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps13, RPS13, GW7_16844 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5B0I4
#4: Protein Small ribosomal subunit protein uS17 / 40S ribosomal protein S11


Mass: 18468.826 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS11, Rps11 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J0Q6
#5: Protein Small ribosomal subunit protein eS1


Mass: 25017.506 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS3A, GW7_01435 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C1K3
#6: Protein Small ribosomal subunit protein uS2 / 37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin ...37 kDa laminin receptor precursor / 37LRP / 37/67 kDa laminin receptor / LRP/LR / 67 kDa laminin receptor / 67LR / Laminin receptor 1 / LamR / Laminin-binding protein precursor p40 / LBP/p40


Mass: 23360.791 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPSA, Rpsa / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6K1L6
#12: Protein Small ribosomal subunit protein uS4 / 40S ribosomal protein S9


Mass: 21266.166 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps9, RPS9, GW7_20173 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5CBG2
#17: Protein Small ribosomal subunit protein uS8 / 40S ribosomal protein S15a


Mass: 14734.357 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS15A, Rps15a, GW7_06692 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5AY31
#22: Protein Small ribosomal subunit protein RACK1 / Receptor for activated C kinase / Receptor of activated protein C kinase 1 / Receptor of activated ...Receptor for activated C kinase / Receptor of activated protein C kinase 1 / Receptor of activated protein kinase C 1


Mass: 34669.113 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: GNB2L1, Rack1 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J231
#24: Protein Small ribosomal subunit protein eS10 / 40S ribosomal protein S10


Mass: 11773.953 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS10, Rps10 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6IZJ7
#26: Protein Small ribosomal subunit protein uS13 / 40S ribosomal protein S18


Mass: 16170.774 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS18, Rps18 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0N8ESM4
#27: Protein Small ribosomal subunit protein uS14 / 40S ribosomal protein S29


Mass: 6364.426 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS29, Rps29 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6JEN2
#28: Protein Small ribosomal subunit protein eS17 / 40S ribosomal protein S17


Mass: 14578.988 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS17, Rps17, GW7_09434 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5B1R5
#30: Protein Small ribosomal subunit protein eS19 / 40S ribosomal protein S19


Mass: 15668.050 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps19, RPS19, GW7_15419 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5AZQ1
#35: Protein Small ribosomal subunit protein uS7 / 40S ribosomal protein S5


Mass: 21525.941 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps5, GW7_05598 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5B7K7
#36: Protein Small ribosomal subunit protein uS9 / 40S ribosomal protein S16


Mass: 15975.753 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPS16, Rps16, GW7_14974 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BJD7

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Protein , 1 types, 1 molecules Ae

#11: Protein Ubiquitin-like protein FUBI


Mass: 6668.938 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Fau, FAU, GW7_20638 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5B6L6

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RNA chain , 5 types, 6 molecules B2A5A6A7A8BC

#19: RNA chain 18S ribosomal RNA


Mass: 600151.188 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#78: RNA chain 28S ribosomal RNA


Mass: 1259862.750 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#79: RNA chain 5S ribosomal RNA


Mass: 38998.078 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat) / References: GenBank: 2261261469
#80: RNA chain 5.8S ribosomal RNA


Mass: 50449.812 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#81: RNA chain E-tRNA


Mass: 24231.510 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)

+
60S ribosomal protein ... , 27 types, 27 molecules CRCzCOCLCMCaCICDCQCSCPCYCZCrChCBCFCcCeCfCiCCCnCoCHCECG

#20: Protein 60S ribosomal protein L19


Mass: 18006.602 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#37: Protein 60S ribosomal protein L10a


Mass: 24879.422 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#38: Protein 60S ribosomal protein L13a


Mass: 23502.217 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#39: Protein 60S ribosomal protein L13


Mass: 24190.484 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#41: Protein 60S ribosomal protein L14


Mass: 16254.391 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#42: Protein 60S ribosomal protein L27a


Mass: 16473.340 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#44: Protein 60S ribosomal protein L10-like


Mass: 24439.754 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#45: Protein 60S ribosomal protein L5


Mass: 33535.625 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#46: Protein 60S ribosomal protein L18


Mass: 21687.676 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#48: Protein 60S ribosomal protein L18a


Mass: 20677.316 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#50: Protein 60S ribosomal protein L17


Mass: 17628.514 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#53: Protein 60S ribosomal protein L26


Mass: 15760.589 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#54: Protein 60S ribosomal protein L27


Mass: 15704.635 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL27, Rpl27, GW7_07845 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BN91
#55: Protein 60S ribosomal protein L28


Mass: 15784.622 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#56: Protein 60S ribosomal protein L35


Mass: 14593.624 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#58: Protein 60S ribosomal protein L3


Mass: 45564.379 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#59: Protein 60S ribosomal protein L7


Mass: 27163.475 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#60: Protein 60S ribosomal protein L30


Mass: 11141.092 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#62: Protein 60S ribosomal protein L32


Mass: 15655.716 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl32, GW7_07994 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BYD8
#63: Protein 60S ribosomal protein L35a


Mass: 12433.548 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#65: Protein 60S ribosomal protein L36


Mass: 12046.592 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl36, RPL36, GW7_20493 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C0Q6
#69: Protein 60S ribosomal protein L4


Mass: 41683.070 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#71: Protein/peptide 60S ribosomal protein L41


Mass: 3473.451 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL41 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J950
#73: Protein 60S ribosomal protein L36a / 60S ribosomal protein L36a-like


Mass: 12345.776 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl36al, LOC101699122, RPL36AL, GW7_12199 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BW65
#75: Protein 60S ribosomal protein L9


Mass: 21770.355 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#76: Protein 60S ribosomal protein L6


Mass: 30020.932 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)
#77: Protein 60S ribosomal protein L7a


Mass: 28039.182 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Production host: Heterocephalus glaber (naked mole-rat)

-
Large ribosomal subunit protein ... , 13 types, 13 molecules CWCVCACTCUCXCbCdCgCkClCpCJ

#21: Protein Large ribosomal subunit protein eL24 / 60S ribosomal protein L24


Mass: 14476.975 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl24, RPL24, GW7_00838 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C020
#40: Protein Large ribosomal subunit protein uL14 / 60S ribosomal protein L23


Mass: 14116.556 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl23, RPL23, GW7_13069 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BK67
#47: Protein Large ribosomal subunit protein uL2 / 60S ribosomal protein L8


Mass: 27843.564 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL8 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6J3A4
#49: Protein Large ribosomal subunit protein eL21 / 60S ribosomal protein L21


Mass: 18478.791 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL21, Rpl21, GW7_12528 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5ATV5
#51: Protein Large ribosomal subunit protein eL22 / 60S ribosomal protein L22


Mass: 13098.869 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: GW7_09159 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C569
#52: Protein Large ribosomal subunit protein uL23 / 60S ribosomal protein L23a


Mass: 14114.720 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: GW7_08185 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BVI3
#57: Protein Large ribosomal subunit protein eL29 / 60S ribosomal protein L29


Mass: 9077.759 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: GW7_07753 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5AXN2
#61: Protein Large ribosomal subunit protein eL31 / 60S ribosomal protein L31


Mass: 13250.433 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL31, Rpl31 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0N8ESF6
#64: Protein Large ribosomal subunit protein eL34 / 60S ribosomal protein L34


Mass: 12994.621 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl34, RPL34, GW7_17859 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5AQL2
#67: Protein Large ribosomal subunit protein eL38 / 60S ribosomal protein L38


Mass: 8107.752 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL38, Rpl38 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6IYP5
#68: Protein/peptide Large ribosomal subunit protein eL39 / 60S ribosomal protein L39


Mass: 6295.562 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: LOC101707773, Rpl39 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0AAX6NYH3
#72: Protein Large ribosomal subunit protein eL43 / 60S ribosomal protein L37a


Mass: 10097.075 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl37a, GW7_00966 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: E3VX70
#74: Protein Large ribosomal subunit protein uL5 / 60S ribosomal protein L11


Mass: 19213.266 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl11 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0AAX6TFX7

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Ubiquitin-ribosomal protein ... , 2 types, 2 molecules AfCm

#34: Protein Ubiquitin-ribosomal protein eS31 fusion protein / Ubiquitin carboxyl extension protein 80


Mass: 8358.903 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rps27a, RPS27A, GW7_15259 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5B8W4
#70: Protein Ubiquitin-ribosomal protein eL40 fusion protein / Ubiquitin A-52 residue ribosomal protein fusion product 1


Mass: 6199.574 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Uba52, GW7_11663 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5C5A6

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Ribosomal protein ... , 2 types, 2 molecules CNCj

#43: Protein Ribosomal protein L15


Mass: 24076.088 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: Rpl15, RPL15, GW7_01043 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: G5BFP9
#66: Protein Ribosomal protein L37


Mass: 10489.371 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Details: Homo sapiens (9606) / Source: (gene. exp.) Heterocephalus glaber (naked mole-rat) / Gene: RPL37, Rpl37 / Production host: Heterocephalus glaber (naked mole-rat) / References: UniProt: A0A0P6JT91

-
Details

Has protein modificationY

-
Experimental details

-
Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

-
Sample preparation

ComponentName: Naked mole-rat Ribosome / Type: RIBOSOME / Entity ID: all / Source: NATURAL
Source (natural)Organism: Heterocephalus glaber (naked mole-rat)
Buffer solutionpH: 7.4
Buffer component
IDConc.NameFormulaBuffer-ID
120 mMHEPESC8H18N2O4S1
2100 mMPotassium acetateCH3CO2K1
310 mMMagnesium acetateC4H6MgO41
42 mMDithiothreitolC4H10O2S21
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
Specimen supportGrid material: COPPER / Grid type: Quantifoil R2/2
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE-PROPANE / Humidity: 100 % / Chamber temperature: 277.15 K

-
Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyModel: TFS KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 3000 nm / Nominal defocus min: 1000 nm
Image recordingElectron dose: 40 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON II (4k x 4k) / Num. of real images: 6147

-
Processing

EM software
IDNameVersionCategory
1EMAN2particle selection
2PHENIX1.21.2_5419model refinement
13RELION3D reconstruction
CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Particle selectionNum. of particles selected: 396782
3D reconstructionResolution: 3.1 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 86927 / Symmetry type: POINT
Atomic model buildingSpace: REAL
Atomic model buildingPDB-ID: 4V6X

4v6x


Accession code: 4V6X / Source name: PDB / Type: experimental model
RefinementCross valid method: NONE
Stereochemistry target values: GeoStd + Monomer Library + CDL v1.2
Displacement parametersBiso mean: 103.89 Å2
Refine LS restraints
Refine-IDTypeDev idealNumber
ELECTRON MICROSCOPYf_bond_d0.0095235572
ELECTRON MICROSCOPYf_angle_d1.1629345936
ELECTRON MICROSCOPYf_chiral_restr0.053742979
ELECTRON MICROSCOPYf_plane_restr0.009321958
ELECTRON MICROSCOPYf_dihedral_angle_d24.5523101892

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