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Yorodumi- PDB-8z9z: Cryo-EM structure of the insect olfactory receptor OR5-Orco heter... -
+Open data
-Basic information
Entry | Database: PDB / ID: 8z9z | ||||||
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Title | Cryo-EM structure of the insect olfactory receptor OR5-Orco heterocomplex from Acyrthosiphon pisum | ||||||
Components |
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Keywords | MEMBRANE PROTEIN | ||||||
Function / homology | Olfactory receptor, insect / 7tm Odorant receptor / olfactory receptor activity / odorant binding / signal transduction / plasma membrane / 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / Odorant receptor / Odorant receptor Function and homology information | ||||||
Biological species | Acyrthosiphon pisum (pea aphid) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.5 Å | ||||||
Authors | Wang, Y.D. / Qiu, L. / Guan, Z.Y. / Wang, Q. / Wang, G.R. / Yin, P. | ||||||
Funding support | China, 1items
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Citation | Journal: Science / Year: 2024 Title: Structural basis for ligand modulation of insect olfactory receptor OR-Orco heterocomplex Authors: Wang, Y.D. / Yin, P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8z9z.cif.gz | 281.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8z9z.ent.gz | 228.7 KB | Display | PDB format |
PDBx/mmJSON format | 8z9z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8z9z_validation.pdf.gz | 1.5 MB | Display | wwPDB validaton report |
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Full document | 8z9z_full_validation.pdf.gz | 1.5 MB | Display | |
Data in XML | 8z9z_validation.xml.gz | 53 KB | Display | |
Data in CIF | 8z9z_validation.cif.gz | 75.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z9/8z9z ftp://data.pdbj.org/pub/pdb/validation_reports/z9/8z9z | HTTPS FTP |
-Related structure data
Related structure data | 39873MC 8z9aC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 52916.766 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acyrthosiphon pisum (pea aphid) / Gene: Orco / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: A0A1S6J137 #2: Protein | | Mass: 42454.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Acyrthosiphon pisum (pea aphid) / Gene: OR5 / Cell line (production host): HEK293 / Production host: Homo sapiens (human) / References: UniProt: A0A1S6J146 #3: Chemical | ChemComp-PC1 / Has ligand of interest | Y | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: OR-Orco heterocomplex / Type: COMPLEX / Entity ID: #1-#2 / Source: RECOMBINANT |
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Source (natural) | Organism: Acyrthosiphon pisum (pea aphid) |
Source (recombinant) | Organism: Homo sapiens (human) |
Buffer solution | pH: 8 |
Specimen | Conc.: 7.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2200 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
EM software | Name: cryoSPARC / Category: classification | ||||||||||||||||||||||||
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CTF correction | Type: NONE | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.5 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 278564 / Symmetry type: POINT | ||||||||||||||||||||||||
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