+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 8xoi | ||||||
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タイトル | Cryo-EM structure of GPR30-Gq complex structure in the presence of fulvestrant | ||||||
要素 |
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キーワード | MEMBRANE PROTEIN/IMMUNE SYSTEM / GPCR / estrogen / GPR30 / Gq / MEMBRANE PROTEIN / MEMBRANE PROTEIN-IMMUNE SYSTEM complex | ||||||
機能・相同性 | 機能・相同性情報 negative regulation of leukocyte activation / nuclear fragmentation involved in apoptotic nuclear change / positive regulation of cardiac vascular smooth muscle cell differentiation / negative regulation of cell cycle process / positive regulation of inositol trisphosphate biosynthetic process / keratin filament / positive regulation of uterine smooth muscle contraction / negative regulation of lipid biosynthetic process / apoptotic chromosome condensation / steroid hormone binding ...negative regulation of leukocyte activation / nuclear fragmentation involved in apoptotic nuclear change / positive regulation of cardiac vascular smooth muscle cell differentiation / negative regulation of cell cycle process / positive regulation of inositol trisphosphate biosynthetic process / keratin filament / positive regulation of uterine smooth muscle contraction / negative regulation of lipid biosynthetic process / apoptotic chromosome condensation / steroid hormone binding / positive regulation of G protein-coupled receptor signaling pathway / cellular response to mineralocorticoid stimulus / positive regulation of endothelial cell apoptotic process / positive regulation of extrinsic apoptotic signaling pathway / dendritic spine membrane / positive regulation of neurotransmitter secretion / cellular response to peptide hormone stimulus / positive regulation of neurogenesis / positive regulation of epidermal growth factor receptor signaling pathway / G protein-coupled estrogen receptor activity / negative regulation of fat cell differentiation / dendritic spine head / nuclear estrogen receptor activity / presynaptic active zone / negative regulation of vascular associated smooth muscle cell proliferation / positive regulation of release of cytochrome c from mitochondria / positive regulation of cysteine-type endopeptidase activity involved in apoptotic process / steroid hormone receptor signaling pathway / neuronal action potential / negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / nuclear receptor-mediated steroid hormone signaling pathway / axon terminus / regulation of cytosolic calcium ion concentration / steroid binding / hippocampal mossy fiber to CA3 synapse / Peptide ligand-binding receptors / positive regulation of release of sequestered calcium ion into cytosol / dendritic shaft / cellular response to estradiol stimulus / G protein-coupled receptor activity / mitochondrial membrane / cellular response to glucose stimulus / positive regulation of protein localization to plasma membrane / Olfactory Signaling Pathway / Activation of the phototransduction cascade / cytoplasmic vesicle membrane / G beta:gamma signalling through PLC beta / Presynaptic function of Kainate receptors / Thromboxane signalling through TP receptor / G-protein activation / trans-Golgi network / G protein-coupled acetylcholine receptor signaling pathway / Activation of G protein gated Potassium channels / Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits / adenylate cyclase-activating G protein-coupled receptor signaling pathway / positive regulation of insulin secretion / Prostacyclin signalling through prostacyclin receptor / Glucagon signaling in metabolic regulation / G beta:gamma signalling through CDC42 / recycling endosome / negative regulation of ERK1 and ERK2 cascade / ADP signalling through P2Y purinoceptor 12 / G beta:gamma signalling through BTK / Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1) / Sensory perception of sweet, bitter, and umami (glutamate) taste / photoreceptor disc membrane / Adrenaline,noradrenaline inhibits insulin secretion / Glucagon-type ligand receptors / Vasopressin regulates renal water homeostasis via Aquaporins / negative regulation of inflammatory response / G alpha (z) signalling events / cellular response to catecholamine stimulus / Glucagon-like Peptide-1 (GLP1) regulates insulin secretion / ADORA2B mediated anti-inflammatory cytokines production / sensory perception of taste / ADP signalling through P2Y purinoceptor 1 / adenylate cyclase-activating dopamine receptor signaling pathway / G beta:gamma signalling through PI3Kgamma / cellular response to prostaglandin E stimulus / vasodilation / Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding / GPER1 signaling / G-protein beta-subunit binding / Inactivation, recovery and regulation of the phototransduction cascade / heterotrimeric G-protein complex / G alpha (12/13) signalling events / extracellular vesicle / signaling receptor complex adaptor activity / Thrombin signalling through proteinase activated receptors (PARs) / GTPase binding / retina development in camera-type eye / presynaptic membrane / phospholipase C-activating G protein-coupled receptor signaling pathway / Ca2+ pathway / nuclear envelope / cellular response to tumor necrosis factor / nervous system development / G alpha (i) signalling events / positive regulation of cytosolic calcium ion concentration / fibroblast proliferation 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) synthetic construct (人工物) | ||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 3.2 Å | ||||||
データ登録者 | Liu, H. / Xu, P. / Xu, H.E. | ||||||
資金援助 | 中国, 1件
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引用 | ジャーナル: Cell Res / 年: 2024 タイトル: Structural and functional evidence that GPR30 is not a direct estrogen receptor. 著者: Heng Liu / Shimeng Guo / Antao Dai / Peiyu Xu / Xin Li / Sijie Huang / Xinheng He / Kai Wu / Xinyue Zhang / Dehua Yang / Xin Xie / H Eric Xu / | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8xoi.cif.gz | 235.2 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8xoi.ent.gz | 180.2 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8xoi.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8xoi_validation.pdf.gz | 1.2 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8xoi_full_validation.pdf.gz | 1.2 MB | 表示 | |
XML形式データ | 8xoi_validation.xml.gz | 37.1 KB | 表示 | |
CIF形式データ | 8xoi_validation.cif.gz | 55.2 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/xo/8xoi ftp://data.pdbj.org/pub/pdb/validation_reports/xo/8xoi | HTTPS FTP |
-関連構造データ
関連構造データ | 38530MC 8xofC 8xogC 8xohC 8xojC M: このデータのモデリングに利用したマップデータ C: 同じ文献を引用 (文献) |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
登録構造単位 |
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1 |
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-要素
#1: タンパク質 | 分子量: 41632.219 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) |
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#2: タンパク質 | 分子量: 38744.371 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GNB1 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: P62873 |
#3: 抗体 | 分子量: 26293.299 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) synthetic construct (人工物) 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) |
#4: タンパク質 | 分子量: 7861.143 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GNG2 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: P59768 |
#5: タンパク質 | 分子量: 42291.457 Da / 分子数: 1 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: GPER1, CEPR, CMKRL2, DRY12, GPER, GPR30 発現宿主: Spodoptera frugiperda (ツマジロクサヨトウ) 参照: UniProt: Q99527 |
-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法 |
-試料調製
構成要素 |
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由来(天然) |
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由来(組換発現) | 生物種: Spodoptera frugiperda (ツマジロクサヨトウ) | ||||||||||||||||||||||||
緩衝液 | pH: 7.4 | ||||||||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | ||||||||||||||||||||||||
急速凍結 | 凍結剤: ETHANE |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: DARK FIELD / 最大 デフォーカス(公称値): 2000 nm / 最小 デフォーカス(公称値): 1000 nm |
撮影 | 電子線照射量: 50 e/Å2 フィルム・検出器のモデル: GATAN K3 BIOQUANTUM (6k x 4k) |
-解析
CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3次元再構成 | 解像度: 3.2 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 536691 / 対称性のタイプ: POINT | ||||||||||||||||||||||||
拘束条件 |
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