[English] 日本語
![](img/lk-miru.gif)
- PDB-8wcp: Cryo-EM structure of human disease-associated P301L Tau amyloid f... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 8wcp | ||||||
---|---|---|---|---|---|---|---|
Title | Cryo-EM structure of human disease-associated P301L Tau amyloid fibril from mouse brain | ||||||
![]() |
| ||||||
![]() | PROTEIN FIBRIL / amyloid | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.28 Å | ||||||
![]() | Liu, K.E. / Zhao, W.B. / Liu, C. | ||||||
Funding support | 1items
| ||||||
![]() | ![]() Title: Cryo-EM structures reveal variant Tau amyloid fibrils between the rTg4510 mouse model and sporadic human tauopathies. Authors: Wanbing Zhao / Kaien Liu / Yun Fan / Qinyue Zhao / Youqi Tao / Mengwei Zhang / Linhua Gan / Wenbo Yu / Bo Sun / Dan Li / Cong Liu / Jian Wang / ![]() | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 58.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 35.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 1 MB | Display | |
Data in XML | ![]() | 26.8 KB | Display | |
Data in CIF | ![]() | 36.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 40089.898 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Protein/peptide | Mass: 869.063 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Protein/peptide | Mass: 783.958 Da / Num. of mol.: 3 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-
Sample preparation
Component | Name: Tau / Type: TISSUE / Entity ID: all / Source: NATURAL |
---|---|
Source (natural) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2400 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 55 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-
Processing
CTF correction | Type: NONE |
---|---|
Helical symmerty | Angular rotation/subunit: -1.36 ° / Axial rise/subunit: 4.78 Å / Axial symmetry: C1 |
3D reconstruction | Resolution: 3.28 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 92188 / Symmetry type: HELICAL |