- PDB-8w77: Human Consensus Olfactory Receptor OR52c in apo state, OR52c only -
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基本情報
登録情報
データベース: PDB / ID: 8w77
タイトル
Human Consensus Olfactory Receptor OR52c in apo state, OR52c only
要素
Human Consensus Olfactory Receptor OR52c in apo state, receptor only,Soluble cytochrome b562
キーワード
MEMBRANE PROTEIN / Olfactory Receptor / G Protein / GPCR / Olfactory GPCR
機能・相同性
Cytochrome b562 / Cytochrome b562 / Cytochrome c/b562 / electron transport chain / periplasmic space / electron transfer activity / iron ion binding / heme binding / Soluble cytochrome b562
ジャーナル: Nat Commun / 年: 2023 タイトル: Understanding the molecular mechanisms of odorant binding and activation of the human OR52 family. 著者: Chulwon Choi / Jungnam Bae / Seonghan Kim / Seho Lee / Hyunook Kang / Jinuk Kim / Injin Bang / Kiheon Kim / Won-Ki Huh / Chaok Seok / Hahnbeom Park / Wonpil Im / Hee-Jung Choi / 要旨: Structural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of ...Structural and mechanistic studies on human odorant receptors (ORs), key in olfactory signaling, are challenging because of their low surface expression in heterologous cells. The recent structure of OR51E2 bound to propionate provided molecular insight into odorant recognition, but the lack of an inactive OR structure limited understanding of the activation mechanism of ORs upon odorant binding. Here, we determined the cryo-electron microscopy structures of consensus OR52 (OR52), a representative of the OR52 family, in the ligand-free (apo) and octanoate-bound states. The apo structure of OR52 reveals a large opening between transmembrane helices (TMs) 5 and 6. A comparison between the apo and active structures of OR52 demonstrates the inward and outward movements of the extracellular and intracellular segments of TM6, respectively. These results, combined with molecular dynamics simulations and signaling assays, shed light on the molecular mechanisms of odorant binding and activation of the OR52 family.