Type: D-saccharide, beta linking / Mass: 221.208 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Formula: C8H15NO6
Identifier
Type
Program
DGlcpNAcb
CONDENSED IUPAC CARBOHYDRATE SYMBOL
GMML 1.0
N-acetyl-b-D-glucopyranosamine
COMMON NAME
GMML 1.0
b-D-GlcpNAc
IUPAC CARBOHYDRATE SYMBOL
PDB-CARE 1.0
GlcNAc
SNFG CARBOHYDRATE SYMBOL
GMML 1.0
Has ligand of interest
N
-
Experimental details
-
Experiment
Experiment
Method: ELECTRON MICROSCOPY
EM experiment
Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction
-
Sample preparation
Component
ID
Name
Type
Entity ID
Parent-ID
Source
1
C3b-albicin dimeric complex
COMPLEX
#1-#3
0
MULTIPLESOURCES
2
Albicin
COMPLEX
#1
1
RECOMBINANT
3
ComplementC3betachain, ComplementC3balpha' chain
COMPLEX
#2-#3
1
NATURAL
Molecular weight
Experimental value: NO
Source (natural)
ID
Entity assembly-ID
Organism
Ncbi tax-ID
1
2
Anopheles albimanus (mosquito)
7167
2
3
Homo sapiens (human)
9606
Source (recombinant)
Organism: Escherichia coli (E. coli)
Buffer solution
pH: 7.4
Specimen
Conc.: 1.5 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES Details: Complex was formed by incubation of C3b, Factor B, Factor D and albicin in vitro and was monodisperse
Specimen support
Grid material: GOLD / Grid type: C-flat-1.2/1.3
Vitrification
Cryogen name: ETHANE
-
Electron microscopy imaging
Microscopy
Model: TFS GLACIOS
Electron gun
Electron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
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