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- PDB-8tga: Complex of NPR1 ectodomain and REGN5381 Fab in an active-like sta... -
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Open data
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Basic information
Entry | Database: PDB / ID: 8tga | ||||||
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Title | Complex of NPR1 ectodomain and REGN5381 Fab in an active-like state with no ANP bound | ||||||
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![]() | IMMUNE SYSTEM / Atrial natriuretic peptide / antibody / activator / pulmonary hypertension | ||||||
Function / homology | ![]() ANPR-A receptor complex / natriuretic peptide receptor activity / positive regulation of cGMP-mediated signaling / body fluid secretion / receptor guanylyl cyclase signaling pathway / positive regulation of renal sodium excretion / peptide receptor activity / guanylate cyclase / cGMP biosynthetic process / guanylate cyclase activity ...ANPR-A receptor complex / natriuretic peptide receptor activity / positive regulation of cGMP-mediated signaling / body fluid secretion / receptor guanylyl cyclase signaling pathway / positive regulation of renal sodium excretion / peptide receptor activity / guanylate cyclase / cGMP biosynthetic process / guanylate cyclase activity / Physiological factors / regulation of vascular permeability / hormone binding / positive regulation of urine volume / G protein-coupled peptide receptor activity / adenylate cyclase activity / dopamine metabolic process / cGMP-mediated signaling / peptide hormone binding / blood vessel diameter maintenance / negative regulation of angiogenesis / negative regulation of smooth muscle cell proliferation / negative regulation of cell growth / regulation of blood pressure / cell surface receptor signaling pathway / receptor complex / protein kinase activity / GTP binding / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.65 Å | ||||||
![]() | Franklin, M.C. / Romero Hernandez, A. | ||||||
Funding support | 1items
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![]() | ![]() Title: Complex of NPR1 ectodomain and REGN5381 Fab in an active-like state with no ANP bound Authors: Franklin, M.C. / Romero Hernandez, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 352.2 KB | Display | ![]() |
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PDB format | ![]() | 240.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 56 KB | Display | |
Data in CIF | ![]() | 82.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 41234MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 52263.723 Da / Num. of mol.: 2 / Fragment: ectodomain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Antibody | Mass: 23323.898 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Antibody | Mass: 24818.770 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Active-like complex of NPR1 ectodomain and REGN5381 Fab, with no ANP present Type: COMPLEX / Entity ID: all / Source: MULTIPLE SOURCES |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7.5 |
Specimen | Conc.: 1.6 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2500 nm / Nominal defocus min: 1000 nm |
Image recording | Electron dose: 41 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
EM software |
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||
Particle selection | Num. of particles selected: 1312179 | ||||||||||||
Symmetry | Point symmetry: C1 (asymmetric) | ||||||||||||
3D reconstruction | Resolution: 3.65 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 495330 / Symmetry type: POINT | ||||||||||||
Refinement | Cross valid method: NONE |