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- PDB-8t9d: CryoEM structure of TR-TRAP -

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Basic information

Entry
Database: PDB / ID: 8t9d
TitleCryoEM structure of TR-TRAP
Components
  • (Mediator of RNA polymerase II transcription subunit ...) x 25
  • Unknown Peptide
KeywordsSTRUCTURAL PROTEIN / TR-TRAP / mediator / CKM module
Function / homology
Function and homology information


positive regulation of mediator complex assembly / positive regulation of T cell extravasation / CKM complex / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development ...positive regulation of mediator complex assembly / positive regulation of T cell extravasation / CKM complex / negative regulation of smooth muscle cell differentiation / enucleate erythrocyte development / positive regulation of type II interferon-mediated signaling pathway / regulation of RNA biosynthetic process / androgen biosynthetic process / positive regulation of G0 to G1 transition / retinal pigment epithelium development / mammary gland branching involved in thelarche / G0 to G1 transition / thyroid hormone receptor signaling pathway / core mediator complex / regulation of vitamin D receptor signaling pathway / ventricular trabecula myocardium morphogenesis / thyroid hormone generation / nuclear retinoic acid receptor binding / mediator complex / positive regulation of keratinocyte differentiation / Generic Transcription Pathway / embryonic heart tube development / cellular response to thyroid hormone stimulus / positive regulation of hepatocyte proliferation / embryonic hindlimb morphogenesis / triglyceride homeostasis / nuclear vitamin D receptor binding / peroxisome proliferator activated receptor binding / positive regulation of chromatin binding / limb development / lens development in camera-type eye / positive regulation of intracellular estrogen receptor signaling pathway / embryonic hemopoiesis / nuclear thyroid hormone receptor binding / megakaryocyte development / histone acetyltransferase binding / cellular response to steroid hormone stimulus / cellular response to hepatocyte growth factor stimulus / cortical actin cytoskeleton / termination of RNA polymerase II transcription / RSV-host interactions / LBD domain binding / epithelial cell proliferation involved in mammary gland duct elongation / fat cell differentiation / skeletal muscle cell differentiation / mammary gland branching involved in pregnancy / somatic stem cell population maintenance / monocyte differentiation / blastocyst development / general transcription initiation factor binding / negative regulation of neuron differentiation / hematopoietic stem cell differentiation / embryonic placenta development / negative regulation of keratinocyte proliferation / positive regulation of transcription initiation by RNA polymerase II / animal organ regeneration / erythrocyte development / nuclear retinoid X receptor binding / nuclear receptor-mediated steroid hormone signaling pathway / negative regulation of fibroblast proliferation / ubiquitin ligase complex / RNA polymerase II preinitiation complex assembly / keratinocyte differentiation / cellular response to epidermal growth factor stimulus / peroxisome proliferator activated receptor signaling pathway / RORA activates gene expression / lactation / Regulation of lipid metabolism by PPARalpha / BMAL1:CLOCK,NPAS2 activates circadian gene expression / Activation of gene expression by SREBF (SREBP) / positive regulation of erythrocyte differentiation / nuclear receptor coactivator activity / liver development / cholesterol homeostasis / promoter-specific chromatin binding / nuclear estrogen receptor binding / nuclear receptor binding / transcription initiation at RNA polymerase II promoter / positive regulation of transcription elongation by RNA polymerase II / transcription coregulator activity / Heme signaling / mRNA transcription by RNA polymerase II / Transcriptional activation of mitochondrial biogenesis / cell morphogenesis / brain development / PPARA activates gene expression / Cytoprotection by HMOX1 / chromatin DNA binding / transcription coactivator binding / DNA-directed 5'-3' RNA polymerase activity / Transcriptional regulation of white adipocyte differentiation / Nuclear Receptor transcription pathway / protein import into nucleus / transcription corepressor activity / ubiquitin protein ligase activity / Circadian Clock / actin binding / angiogenesis / DNA-binding transcription factor binding / Estrogen-dependent gene expression
Similarity search - Function
MID domain of medPIWI / MID domain of medPIWI / Mediator complex subunit Med13, C-terminal / Mediator complex subunit 13 C-terminal domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 ...MID domain of medPIWI / MID domain of medPIWI / Mediator complex subunit Med13, C-terminal / Mediator complex subunit 13 C-terminal domain / Mediator complex, subunit Med25, PTOV domain / Mediator complex, subunit Med25, synapsin 1 / Mediator of RNA polymerase II transcription subunit 25, von Willebrand factor type A domain / Mediator complex subunit 25, PTOV domain superfamily / Mediator complex subunit 25 PTOV activation and synapsin 2 / Mediator complex subunit 25 synapsin 1 / Mediator complex subunit 25 von Willebrand factor type A / Mediator complex, subunit Med29, metazoa / Mediator complex, subunit Med24, / : / Mediator complex subunit 24 N-terminal / Mediator complex subunit 29 / Mediator complex subunit 16, central helical bridge / Mediator complex, subunit Med23 / Mediator complex subunit 23 / Mediator of RNA polymerase II transcription subunit 15 / : / : / ARC105/Med15 mediator subunit N-terminal KIX domain / ARC105/Med15 mediator subunit central domain / ARC105/Med15 mediator subunit C-terminal domain / Mediator complex, subunit Med6, metazoa/plant / Mediator complex, subunit Med30, metazoa / Mediator complex, subunit Med28 / Mediator of RNA polymerase II transcription subunit 9, metazoan / Mediator complex subunit 30 / Mediator complex subunit 28 / Mediator complex, subunit Med27 / Mediator complex subunit 27 / : / : / Mediator complex subunit 16, C-terminal / Mediator complex, subunit Med16 / Mediator complex subunit 16, N-terminal / Mediator complex, subunit Med10 / Transcription factor subunit Med10 of Mediator complex / Mediator of RNA polymerase II transcription subunit 9 / RNA polymerase II transcription mediator complex subunit 9 / Mediator complex, subunit Med4 / Vitamin-D-receptor interacting Mediator subunit 4 / Mediator complex, subunit Med31 / Mediator complex, subunit Med21 / Mediator of RNA polymerase II, subunit Med31 superfamily / Mediator complex, subunit Med7 superfmaily / SOH1 / Subunit 21 of Mediator complex / Mediator complex, subunit Med7 / Mediator complex, subunit Med7/Med21-like / MED7 protein / Mediator complex, subunit Med20 / Mediator complex, subunit Med14 / TATA-binding related factor (TRF) of subunit 20 of Mediator complex / Mediator complex subunit MED14 / Soluble quinoprotein glucose/sorbosone dehydrogenase / Mediator complex, subunit Med6 / Mediator complex, subunit Med17 / Mediator complex, subunit Med6 superfamily / MED6 mediator sub complex component / Subunit 17 of Mediator complex / Mediator of RNA polymerase II transcription subunit 22 / Mediator complex, subunit Med8, fungi/metazoa / Mediator complex, subunit Med11 / Surfeit locus protein 5 subunit 22 of Mediator complex / Mediator of RNA polymerase II transcription complex subunit 8 / Mediator complex protein / Mediator complex, subunit Med18 / Med18 protein / Mediator complex, subunit Med1 / Mediator of RNA polymerase II transcription subunit 1 / Coactivator CBP, KIX domain superfamily / von Willebrand factor A-like domain superfamily / Trp-Asp (WD) repeats profile. / Trp-Asp (WD) repeats circular profile. / WD domain, G-beta repeat / WD40 repeats / WD40 repeat / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily
Similarity search - Domain/homology
Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 8 ...Mediator of RNA polymerase II transcription subunit 7 / Mediator of RNA polymerase II transcription subunit 14 / Mediator of RNA polymerase II transcription subunit 24 / Mediator of RNA polymerase II transcription subunit 6 / Mediator of RNA polymerase II transcription subunit 21 / Mediator of RNA polymerase II transcription subunit 22 / Mediator of RNA polymerase II transcription subunit 1 / Mediator of RNA polymerase II transcription subunit 27 / Mediator of RNA polymerase II transcription subunit 25 / Mediator of RNA polymerase II transcription subunit 8 / Mediator of RNA polymerase II transcription subunit 30 / Mediator of RNA polymerase II transcription subunit 15 / Mediator of RNA polymerase II transcription subunit 10 / Mediator of RNA polymerase II transcription subunit 18 / Mediator of RNA polymerase II transcription subunit 28 / Mediator of RNA polymerase II transcription subunit 20 / Mediator of RNA polymerase II transcription subunit 4 / Mediator of RNA polymerase II transcription subunit 17 / Mediator of RNA polymerase II transcription subunit 9 / Mediator of RNA polymerase II transcription subunit 29 / Mediator of RNA polymerase II transcription subunit 11 / Mediator of RNA polymerase II transcription subunit 13 / Mediator of RNA polymerase II transcription subunit 23 / Mediator of RNA polymerase II transcription subunit 16 / Mediator of RNA polymerase II transcription subunit 31
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.66 Å
AuthorsZhao, H. / Asturias, F.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)R01-67167 United States
CitationJournal: Mol Cell / Year: 2024
Title: An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II.
Authors: Haiyan Zhao / Jiaqin Li / Yufei Xiang / Sohail Malik / Supriya V Vartak / Giovana M B Veronezi / Natalie Young / McKayla Riney / Jens Kalchschmidt / Andrea Conte / Seol Kyoung Jung / ...Authors: Haiyan Zhao / Jiaqin Li / Yufei Xiang / Sohail Malik / Supriya V Vartak / Giovana M B Veronezi / Natalie Young / McKayla Riney / Jens Kalchschmidt / Andrea Conte / Seol Kyoung Jung / Srinivas Ramachandran / Robert G Roeder / Yi Shi / Rafael Casellas / Francisco J Asturias /
Abstract: The essential Mediator (MED) coactivator complex plays a well-understood role in regulation of basal transcription in all eukaryotes, but the mechanism underlying its role in activator-dependent ...The essential Mediator (MED) coactivator complex plays a well-understood role in regulation of basal transcription in all eukaryotes, but the mechanism underlying its role in activator-dependent transcription remains unknown. We investigated modulation of metazoan MED interaction with RNA polymerase II (RNA Pol II) by antagonistic effects of the MED26 subunit and the CDK8 kinase module (CKM). Biochemical analysis of CKM-MED showed that the CKM blocks binding of the RNA Pol II carboxy-terminal domain (CTD), preventing RNA Pol II interaction. This restriction is eliminated by nuclear receptor (NR) binding to CKM-MED, which enables CTD binding in a MED26-dependent manner. Cryoelectron microscopy (cryo-EM) and crosslinking-mass spectrometry (XL-MS) revealed that the structural basis for modulation of CTD interaction with MED relates to a large intrinsically disordered region (IDR) in CKM subunit MED13 that blocks MED26 and CTD interaction with MED but is repositioned upon NR binding. Hence, NRs can control transcription initiation by priming CKM-MED for MED26-dependent RNA Pol II interaction.
History
DepositionJun 23, 2023Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jul 3, 2024Provider: repository / Type: Initial release
Revision 1.1Jul 17, 2024Group: Data collection / Database references / Category: citation / citation_author / em_admin
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year / _em_admin.last_update

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Mediator of RNA polymerase II transcription subunit 1
B: Mediator of RNA polymerase II transcription subunit 4
C: Mediator of RNA polymerase II transcription subunit 6
D: Mediator of RNA polymerase II transcription subunit 7
E: Mediator of RNA polymerase II transcription subunit 8
F: Mediator of RNA polymerase II transcription subunit 9
G: Mediator of RNA polymerase II transcription subunit 10
H: Mediator of RNA polymerase II transcription subunit 11
I: Mediator of RNA polymerase II transcription subunit 14
J: Mediator of RNA polymerase II transcription subunit 15
K: Mediator of RNA polymerase II transcription subunit 16
L: Mediator of RNA polymerase II transcription subunit 17
M: Mediator of RNA polymerase II transcription subunit 18
O: Mediator of RNA polymerase II transcription subunit 20
P: Mediator of RNA polymerase II transcription subunit 21
Q: Mediator of RNA polymerase II transcription subunit 22
R: Mediator of RNA polymerase II transcription subunit 23
S: Mediator of RNA polymerase II transcription subunit 24
T: Mediator of RNA polymerase II transcription subunit 25
V: Mediator of RNA polymerase II transcription subunit 27
W: Mediator of RNA polymerase II transcription subunit 28
X: Mediator of RNA polymerase II transcription subunit 29
Y: Mediator of RNA polymerase II transcription subunit 30
Z: Mediator of RNA polymerase II transcription subunit 31
a: Unknown Peptide
9: Mediator of RNA polymerase II transcription subunit 13


Theoretical massNumber of molelcules
Total (without water)1,529,69226
Polymers1,529,69226
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author&software
  • Evidence: electron microscopy, not applicable
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1

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Components

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Mediator of RNA polymerase II transcription subunit ... , 25 types, 25 molecules ABCDEFGHIJKLMOPQRSTVWXYZ9

#1: Protein Mediator of RNA polymerase II transcription subunit 1 / Activator-recruited cofactor 205 kDa component / ARC205 / Mediator complex subunit 1 / Peroxisome ...Activator-recruited cofactor 205 kDa component / ARC205 / Mediator complex subunit 1 / Peroxisome proliferator-activated receptor-binding protein / PBP / PPAR-binding protein / Thyroid hormone receptor-associated protein complex 220 kDa component / Trap220 / Thyroid receptor-interacting protein 2 / TR-interacting protein 2 / TRIP-2 / Vitamin D receptor-interacting protein complex component DRIP205 / p53 regulatory protein RB18A


Mass: 168697.328 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: Q15648
#2: Protein Mediator of RNA polymerase II transcription subunit 4 / Activator-recruited cofactor 36 kDa component / ARC36 / Mediator complex subunit 4 / TRAP/SMCC/PC2 ...Activator-recruited cofactor 36 kDa component / ARC36 / Mediator complex subunit 4 / TRAP/SMCC/PC2 subunit p36 subunit / Vitamin D3 receptor-interacting protein complex 36 kDa component / DRIP36


Mass: 29781.424 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: Q9NPJ6
#3: Protein Mediator of RNA polymerase II transcription subunit 6 / Activator-recruited cofactor 33 kDa component / ARC33 / Mediator complex subunit 6 / hMed6 / Renal ...Activator-recruited cofactor 33 kDa component / ARC33 / Mediator complex subunit 6 / hMed6 / Renal carcinoma antigen NY-REN-28


Mass: 28464.627 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / Cell line: HELA / References: UniProt: O75586
#4: Protein Mediator of RNA polymerase II transcription subunit 7 / hMED7 / Activator-recruited cofactor 34 kDa component / ARC34 / Cofactor required for Sp1 ...hMED7 / Activator-recruited cofactor 34 kDa component / ARC34 / Cofactor required for Sp1 transcriptional activation subunit 9 / CRSP complex subunit 9 / Mediator complex subunit 7 / RNA polymerase transcriptional regulation mediator subunit 7 homolog / Transcriptional coactivator CRSP33


Mass: 27285.281 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O43513
#5: Protein Mediator of RNA polymerase II transcription subunit 8 / Activator-recruited cofactor 32 kDa component / ARC32 / Mediator complex subunit 8


Mass: 29112.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96G25
#6: Protein Mediator of RNA polymerase II transcription subunit 9 / Mediator complex subunit 9


Mass: 16422.750 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NWA0
#7: Protein Mediator of RNA polymerase II transcription subunit 10 / Mediator complex subunit 10 / Transformation-related gene 17 protein / TRG-17 / Transformation- ...Mediator complex subunit 10 / Transformation-related gene 17 protein / TRG-17 / Transformation-related gene 20 protein / TRG-20


Mass: 15710.969 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BTT4
#8: Protein Mediator of RNA polymerase II transcription subunit 11 / Mediator complex subunit 11


Mass: 13145.829 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9P086
#9: Protein Mediator of RNA polymerase II transcription subunit 14 / Activator-recruited cofactor 150 kDa component / ARC150 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 150 kDa component / ARC150 / Cofactor required for Sp1 transcriptional activation subunit 2 / CRSP complex subunit 2 / Mediator complex subunit 14 / RGR1 homolog / hRGR1 / Thyroid hormone receptor-associated protein complex 170 kDa component / Trap170 / Transcriptional coactivator CRSP150 / Vitamin D3 receptor-interacting protein complex 150 kDa component / DRIP150


Mass: 160787.594 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O60244
#10: Protein Mediator of RNA polymerase II transcription subunit 15 / Activator-recruited cofactor 105 kDa component / ARC105 / CTG repeat protein 7a / Mediator complex ...Activator-recruited cofactor 105 kDa component / ARC105 / CTG repeat protein 7a / Mediator complex subunit 15 / Positive cofactor 2 glutamine/Q-rich-associated protein / PC2 glutamine/Q-rich-associated protein / TPA-inducible gene 1 protein / TIG-1 / Trinucleotide repeat-containing gene 7 protein


Mass: 86830.102 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96RN5
#11: Protein Mediator of RNA polymerase II transcription subunit 16 / Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone ...Mediator complex subunit 16 / Thyroid hormone receptor-associated protein 5 / Thyroid hormone receptor-associated protein complex 95 kDa component / Trap95 / Vitamin D3 receptor-interacting protein complex 92 kDa component / DRIP92


Mass: 96908.055 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y2X0
#12: Protein Mediator of RNA polymerase II transcription subunit 17 / Activator-recruited cofactor 77 kDa component / ARC77 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 77 kDa component / ARC77 / Cofactor required for Sp1 transcriptional activation subunit 6 / CRSP complex subunit 6 / Mediator complex subunit 17 / Thyroid hormone receptor-associated protein complex 80 kDa component / Trap80 / Transcriptional coactivator CRSP77 / Vitamin D3 receptor-interacting protein complex 80 kDa component / DRIP80


Mass: 72984.047 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NVC6
#13: Protein Mediator of RNA polymerase II transcription subunit 18 / Mediator complex subunit 18 / p28b


Mass: 23693.730 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9BUE0
#14: Protein Mediator of RNA polymerase II transcription subunit 20 / Mediator complex subunit 20 / TRF-proximal protein homolog / hTRFP


Mass: 23243.762 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H944
#15: Protein Mediator of RNA polymerase II transcription subunit 21 / Mediator complex subunit 21 / RNA polymerase II holoenzyme component SRB7 / RNAPII complex ...Mediator complex subunit 21 / RNA polymerase II holoenzyme component SRB7 / RNAPII complex component SRB7 / hSrb7


Mass: 15575.234 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q13503
#16: Protein Mediator of RNA polymerase II transcription subunit 22 / Mediator complex subunit 22 / Surfeit locus protein 5 / Surf-5


Mass: 22243.713 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q15528
#17: Protein Mediator of RNA polymerase II transcription subunit 23 / Activator-recruited cofactor 130 kDa component / ARC130 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 130 kDa component / ARC130 / Cofactor required for Sp1 transcriptional activation subunit 3 / CRSP complex subunit 3 / Mediator complex subunit 23 / Protein sur-2 homolog / hSur-2 / Transcriptional coactivator CRSP130 / Vitamin D3 receptor-interacting protein complex 130 kDa component / DRIP130


Mass: 156652.156 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9ULK4
#18: Protein Mediator of RNA polymerase II transcription subunit 24 / Activator-recruited cofactor 100 kDa component / ARC100 / Cofactor required for Sp1 transcriptional ...Activator-recruited cofactor 100 kDa component / ARC100 / Cofactor required for Sp1 transcriptional activation subunit 4 / CRSP complex subunit 4 / Mediator complex subunit 24 / Thyroid hormone receptor-associated protein 4 / Thyroid hormone receptor-associated protein complex 100 kDa component / Trap100 / hTRAP100 / Vitamin D3 receptor-interacting protein complex 100 kDa component / DRIP100


Mass: 110430.164 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O75448
#19: Protein Mediator of RNA polymerase II transcription subunit 25 / Activator interaction domain-containing protein 1 / Activator-recruited cofactor 92 kDa component / ...Activator interaction domain-containing protein 1 / Activator-recruited cofactor 92 kDa component / ARC92 / Mediator complex subunit 25 / p78


Mass: 78230.344 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q71SY5
#20: Protein Mediator of RNA polymerase II transcription subunit 27 / Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator ...Cofactor required for Sp1 transcriptional activation subunit 8 / CRSP complex subunit 8 / Mediator complex subunit 27 / P37 TRAP/SMCC/PC2 subunit / Transcriptional coactivator CRSP34


Mass: 35478.742 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q6P2C8
#21: Protein Mediator of RNA polymerase II transcription subunit 28 / Endothelial-derived protein 1 / Mediator complex subunit 28 / Merlin and Grb2-interacting ...Endothelial-derived protein 1 / Mediator complex subunit 28 / Merlin and Grb2-interacting cytoskeletal protein / Magicin / Tumor angiogenesis marker EG-1


Mass: 19540.107 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9H204
#22: Protein Mediator of RNA polymerase II transcription subunit 29 / Intersex-like protein / Mediator complex subunit 29


Mass: 21089.801 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9NX70
#23: Protein Mediator of RNA polymerase II transcription subunit 30 / Mediator complex subunit 30 / TRAP/Mediator complex component TRAP25 / Thyroid hormone receptor- ...Mediator complex subunit 30 / TRAP/Mediator complex component TRAP25 / Thyroid hormone receptor-associated protein 6 / Thyroid hormone receptor-associated protein complex 25 kDa component / Trap25


Mass: 20301.229 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q96HR3
#24: Protein Mediator of RNA polymerase II transcription subunit 31 / Mediator complex subunit 31 / Mediator complex subunit SOH1 / hSOH1


Mass: 15826.116 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9Y3C7
#26: Protein Mediator of RNA polymerase II transcription subunit 13 / Activator-recruited cofactor 250 kDa component / ARC250 / Mediator complex subunit 13 / Thyroid ...Activator-recruited cofactor 250 kDa component / ARC250 / Mediator complex subunit 13 / Thyroid hormone receptor-associated protein 1 / Thyroid hormone receptor-associated protein complex 240 kDa component / Trap240 / Vitamin D3 receptor-interacting protein complex component DRIP250 / DRIP250


Mass: 239535.906 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: Q9UHV7

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Protein/peptide , 1 types, 1 molecules a

#25: Protein/peptide Unknown Peptide


Mass: 1720.111 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: mediator complex with transcription factor TR / Type: COMPLEX / Entity ID: all / Source: NATURAL
Source (natural)Organism: Homo sapiens (human)
Buffer solutionpH: 7.9
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Talos Arctica / Image courtesy: FEI Company
MicroscopyModel: FEI TECNAI ARCTICA
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Nominal defocus max: 35000 nm / Nominal defocus min: 800 nm
Image recordingElectron dose: 100 e/Å2 / Film or detector model: GATAN K3 (6k x 4k)

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Processing

CTF correctionType: NONE
3D reconstructionResolution: 4.66 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 31505 / Symmetry type: POINT

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