ジャーナル: Environ Sci Technol / 年: 2024 タイトル: Influence of Amino Acid Substitutions in Capsid Proteins of Coxsackievirus B5 on Free Chlorine and Thermal Inactivation. 著者: Shotaro Torii / Jérôme Gouttenoire / Kiruthika Kumar / Aleksandar Antanasijevic / Tamar Kohn / 要旨: The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions ...The sensitivity of enteroviruses to disinfectants varies among genetically similar variants and coincides with amino acid changes in capsid proteins, although the effect of individual substitutions remains unknown. Here, we employed reverse genetics to investigate how amino acid substitutions in coxsackievirus B5 (CVB5) capsid proteins affect the virus' sensitivity to free chlorine and heat treatment. Of ten amino acid changes observed in CVB5 variants with free chlorine resistance, none significantly reduced the chlorine sensitivity, indicating a minor role of the capsid composition in chlorine sensitivity of CVB5. Conversely, a subset of these amino acid changes located at the C-terminal region of viral protein 1 led to reduced heat sensitivity. Cryo-electron microscopy revealed that these changes affect the assembly of intermediate viral states (altered and empty particles), suggesting that the mechanism for reduced heat sensitivity could be related to improved molecular packing of CVB5, resulting in greater stability or altered dynamics of virus uncoating during infection.
A: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1 B: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1 C: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
A: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1 B: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1 C: Coxsackievirus B5 (mutant CVB5F.cas.genogroupB) in particle A state - VP1
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登録者が定義した集合体
根拠: 電子顕微鏡法, Icosahedral symmetry operators are in the header of uploaded PDB file.
名称: Coxsackievirus B5 / タイプ: VIRUS / 詳細: Particle A state of the CVB5 virion capsid / Entity ID: all / 由来: RECOMBINANT
由来(天然)
生物種: Coxsackievirus B5 (コクサッキーウイルス)
由来(組換発現)
生物種: Homo sapiens (ヒト) / 細胞: BGMK
ウイルスについての詳細
中空か: NO / エンベロープを持つか: NO / 単離: STRAIN / タイプ: VIRION
天然宿主
生物種: Homo sapiens
ウイルス殻
名称: Capsid VP1-4 / 直径: 300 nm
緩衝液
pH: 7.5 / 詳細: TBS
緩衝液成分
ID
濃度
名称
式
Buffer-ID
1
25mM
Tris-HCl
Tris-HCl
1
2
150mM
Sodiumchloride
NaCl
1
試料
濃度: 4.5 mg/ml / 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES 詳細: Inactivated by formaldehyde. Purified using a combination of sucrose gradient and size-exclusion chromatography.
急速凍結
装置: FEI VITROBOT MARK IV / 凍結剤: ETHANE / 湿度: 100 % / 凍結前の試料温度: 283 K 詳細: 3ul of sample applied. Blotting time varied. Blotting force = 0. Total blots = 1.