+
Open data
-
Basic information
Entry | Database: PDB / ID: 8p4i | ||||||
---|---|---|---|---|---|---|---|
Title | Cyanide dihydratase from Bacillus pumilus C1 | ||||||
![]() | Cyanide dihydratase | ||||||
![]() | HYDROLASE / Cyanide degrading enzyme | ||||||
Function / homology | ![]() nitrilase activity / detoxification of nitrogen compound / nitrile hydratase activity Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.83 Å | ||||||
![]() | Mulelu, A.E. / Reitz, J. / van Rooyen, J.M. / Scheffer, M. / Frangakis, A.S. / Dlamini, L.S. / Woodward, J.D. / Benedik, M.J. / Sewell, B.T. | ||||||
Funding support | ![]()
| ||||||
![]() | ![]() Title: The Role of Histidine Residues in the Oligomerization of Cyanide Dihydratase from Bacillus pumilus C1 Authors: Mulelu, A.E. / Reitz, J. / van Rooyen, J. / Scheffer, M. / Frangakis, A.S. / Dlamini, L.S. / Woodward, J.D. / Benedik, M.J. / Sewell, B.T. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 900.9 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 765.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
---|
-Related structure data
Related structure data | ![]() 17409MC ![]() 8c5iC C: citing same article ( M: map data used to model this data |
---|---|
Similar structure data | Similarity search - Function & homology ![]() |
-
Links
-
Assembly
Deposited unit | ![]()
|
---|---|
1 |
|
-
Components
#1: Protein | Mass: 35941.418 Da / Num. of mol.: 17 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
---|
-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
---|---|
EM experiment | Aggregation state: FILAMENT / 3D reconstruction method: helical reconstruction |
-
Sample preparation
Component | Name: Wildtype cyanide dihydratase from Bacillus pumilus C1 / Type: COMPLEX Details: Homo-oligomeric 18mer expressed recombinantly without a purification tag. Entity ID: all / Source: RECOMBINANT |
---|---|
Molecular weight | Value: 0.666 kDa/nm / Experimental value: YES |
Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 5.4 |
Buffer component | Conc.: 50 mM / Name: citrate / Formula: C6H8O7 |
Specimen | Conc.: 0.2 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: COPPER / Grid type: Quantifoil R2/2 |
Vitrification | Instrument: FEI VITROBOT MARK I / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 277.15 K |
-
Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
---|---|
Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: OTHER / Nominal defocus max: 2500 nm / Nominal defocus min: 500 nm |
Specimen holder | Cryogen: NITROGEN |
Image recording | Electron dose: 59.4 e/Å2 / Detector mode: COUNTING / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
-
Processing
EM software |
| |||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | |||||||||||||||||||||||||
Helical symmerty | Angular rotation/subunit: -77 ° / Axial rise/subunit: 16.9 Å / Axial symmetry: C2 | |||||||||||||||||||||||||
3D reconstruction | Resolution: 3.83 Å / Resolution method: FSC 0.5 CUT-OFF / Num. of particles: 120943 / Symmetry type: HELICAL | |||||||||||||||||||||||||
Atomic model building | Protocol: AB INITIO MODEL / Space: REAL | |||||||||||||||||||||||||
Refine LS restraints |
|