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Open data
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Basic information
Entry | Database: PDB / ID: 8ojg | ||||||
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Title | Structure of the MlaCD complex (2:6 stoichiometry) | ||||||
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![]() | LIPID TRANSPORT / Outer membrane / phospholipids / gram-negative bacteria | ||||||
Function / homology | ![]() phospholipid transporter activity / phospholipid binding / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 4.38 Å | ||||||
![]() | Wotherspoon, P. / Bui, S. / Sridhar, P. / Bergeron, J.R.C. / Knowles, T.J. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The structure of the MlaC-MlaD complex reveals novel insights into periplasmic phospholipid transport processes Authors: Wotherspoon, P. / Bui, S. / Sridhar, P. / Ratkeviciute, G. / Colburn, J. / Johnston, H. / Cooper, B.F. / Bryant, J.A. / Hughes, G.W. / Stransfeld, P.J. / Bergeron, J.R.C. / Knowles, T.J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 235.7 KB | Display | ![]() |
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PDB format | ![]() | 182.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 42.4 KB | Display | |
Data in CIF | ![]() | 60.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 16913MC ![]() 8oj4C M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 19593.133 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 23989.559 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Gene: mlaC, ttg2, A9X72_02830, AC065_15340, ACN81_18440, ACU57_14440, AM464_08590, B6R12_000281, B6R15_000958, BANRA_04208, BB540_06745, BG944_002131, BGM66_001562, BHS81_19205, BJI68_19310, BJJ90_ ...Gene: mlaC, ttg2, A9X72_02830, AC065_15340, ACN81_18440, ACU57_14440, AM464_08590, B6R12_000281, B6R15_000958, BANRA_04208, BB540_06745, BG944_002131, BGM66_001562, BHS81_19205, BJI68_19310, BJJ90_02765, BK292_10215, BK383_15970, BKL28_000244, BMT50_12830, BMT91_11605, BTQ06_11875, BUO55_000263, BvCmsKKP061_00889, BvCmsSIP010_02570, BXT93_02555, C0P57_001815, C1Q91_000884, C5N07_18065, CA593_10340, CCS08_10440, CDL36_04615, CF22_002953, CG692_07305, CG831_001214, CR538_03065, CRM83_23495, CTR35_000707, CV83915_02906, CWS33_08380, CX938_002795, D6T60_15395, D9H94_03530, DIV22_02970, DS732_23700, DTL43_04300, E0I52_08600, E4K51_05485, E5H86_10400, E6D34_05750, EAI46_04525, EC95NR1_02576, EIZ93_11795, EN85_002300, ERS139208_02240, FJQ40_07020, FKO60_06245, FOI11_019790, FOI11_03390, FPS11_10310, FTV92_21225, FV293_08370, FVB16_04435, FWK02_19640, G3V95_08780, G4A38_08310, G4A47_07700, G9448_17585, GAI89_03480, GAJ12_14365, GIB53_00725, GKF89_14370, GNW61_09670, GOP14_001713, GOP25_03385, GP965_13290, GP979_08050, GQA06_06925, GQM04_01370, GQM13_09425, GQM17_12880, GQM21_01470, GRW05_12305, GRW24_07810, GRW56_06765, GRW57_06230, GUC01_10050, HHH44_002790, HKA49_001544, HLQ92_10680, HLZ50_05480, HMV95_13990, HV209_15220, HVW43_19180, HVY77_03000, HX136_03665, I6H00_21450, I6H02_11475, J0541_003227, J4S20_002658, J5U05_001208, JFD_04121, JNP96_24765, KQ660_11265, NCTC10090_03636, NCTC10764_03981, NCTC10865_00785, NCTC10974_00681, NCTC11126_02903, NCTC11127_03108, NCTC11181_02846, NCTC11341_01769, NCTC4450_02582, NCTC8179_06069, NCTC8333_00602, NCTC8500_00485, NCTC8622_00354, NCTC8960_03187, NCTC9050_03717, NCTC9071_00594, NCTC9111_00973, NCTC9117_00876, NCTC9706_02843, NEP60_07295, RG28_21080, SAMEA3472044_03307, SAMEA3752557_01850, SAMEA3753106_02392, TUM18780_05300, WR15_24435 Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: MlaCD (2:6 stoichiometry) / Type: COMPLEX / Entity ID: #2, #1 / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 500 nm |
Image recording | Electron dose: 40 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
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3D reconstruction | Resolution: 4.38 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 58259 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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