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Open data
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Basic information
Entry | Database: PDB / ID: 8j2g | ||||||
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Title | Human ZnT1-D47N/D255N mutant | ||||||
![]() | Proton-coupled zinc antiporter SLC30A1 | ||||||
![]() | TRANSPORT PROTEIN / zinc exporter | ||||||
Function / homology | ![]() negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / detoxification of cadmium ion / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion import into organelle / zinc:proton antiporter activity / glutamatergic postsynaptic density / regulation of postsynaptic density protein 95 clustering ...negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / detoxification of cadmium ion / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion import into organelle / zinc:proton antiporter activity / glutamatergic postsynaptic density / regulation of postsynaptic density protein 95 clustering / zinc ion transmembrane transporter activity / zinc ion transport / zinc ion transmembrane transport / negative regulation of calcium ion import / negative regulation of neurotransmitter secretion / positive regulation of dendritic spine morphogenesis / intracellular zinc ion homeostasis / calcium ion import / postsynaptic density, intracellular component / calcium channel inhibitor activity / T-tubule / postsynaptic density membrane / cytoplasmic vesicle membrane / Schaffer collateral - CA1 synapse / intracellular calcium ion homeostasis / basolateral plasma membrane / nuclear membrane / in utero embryonic development / defense response to bacterium / dendrite / Golgi apparatus / endoplasmic reticulum / membrane / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||
![]() | Sun, L. / Liu, X. / Sun, C. / He, B. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structure of the human ZnT1-D47N/D255N double mutant Authors: Sun, L. / Liu, X. / Sun, C. / He, B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.5 KB | Display | ![]() |
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PDB format | ![]() | 95.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 35949MC M: map data used to model this data C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Components
#1: Protein | Mass: 55360.633 Da / Num. of mol.: 2 / Mutation: D47N,D255N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: Human ZnT1-D47N/D255N mutant homodimer / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Molecular weight | Experimental value: NO |
Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() |
Buffer solution | pH: 7.4 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Specimen support | Grid material: GOLD / Grid mesh size: 300 divisions/in. / Grid type: Quantifoil |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: ![]() |
Electron lens | Mode: DIFFRACTION / Nominal defocus max: 2300 nm / Nominal defocus min: 1500 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
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Processing
EM software | Name: PHENIX / Version: 1.13_2998: / Category: model refinement |
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CTF correction | Type: NONE |
3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 550472 / Symmetry type: POINT |