+Open data
-Basic information
Entry | Database: EMDB / ID: EMD-35949 | |||||||||
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Title | Human ZnT1-D47N/D255N mutant | |||||||||
Map data | ||||||||||
Sample |
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Keywords | zinc exporter / TRANSPORT PROTEIN | |||||||||
Function / homology | Function and homology information negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / zinc ion import into organelle / detoxification of cadmium ion / zinc:proton antiporter activity / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion transmembrane transporter activity / zinc ion transport ...negative regulation of zinc ion transmembrane import / zinc export across plasma membrane / detoxification of zinc ion / zinc ion import into organelle / detoxification of cadmium ion / zinc:proton antiporter activity / Zinc efflux and compartmentalization by the SLC30 family / cadmium ion transmembrane transport / zinc ion transmembrane transporter activity / zinc ion transport / zinc ion transmembrane transport / negative regulation of calcium ion import / negative regulation of neurotransmitter secretion / intracellular zinc ion homeostasis / calcium ion import / calcium channel inhibitor activity / T-tubule / postsynaptic density membrane / Schaffer collateral - CA1 synapse / cytoplasmic vesicle membrane / intracellular calcium ion homeostasis / basolateral plasma membrane / nuclear membrane / in utero embryonic development / defense response to bacterium / Golgi apparatus / endoplasmic reticulum / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||
Biological species | Homo sapiens (human) | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
Authors | Sun L / Liu X / Sun C / He B | |||||||||
Funding support | China, 1 items
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Citation | Journal: To Be Published Title: Structure of the human ZnT1-D47N/D255N double mutant Authors: Sun L / Liu X / Sun C / He B | |||||||||
History |
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-Structure visualization
Supplemental images |
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-Downloads & links
-EMDB archive
Map data | emd_35949.map.gz | 28.6 MB | EMDB map data format | |
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Header (meta data) | emd-35949-v30.xml emd-35949.xml | 12.7 KB 12.7 KB | Display Display | EMDB header |
Images | emd_35949.png | 121.6 KB | ||
Filedesc metadata | emd-35949.cif.gz | 5.2 KB | ||
Others | emd_35949_half_map_1.map.gz emd_35949_half_map_2.map.gz | 27.9 MB 27.9 MB | ||
Archive directory | http://ftp.pdbj.org/pub/emdb/structures/EMD-35949 ftp://ftp.pdbj.org/pub/emdb/structures/EMD-35949 | HTTPS FTP |
-Related structure data
Related structure data | 8j2gMC M: atomic model generated by this map C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
EMDB pages | EMDB (EBI/PDBe) / EMDataResource |
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-Map
File | Download / File: emd_35949.map.gz / Format: CCP4 / Size: 30.5 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) | ||||||||||||||||||||||||||||||||||||
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Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.1 Å | ||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Half map: #1
File | emd_35949_half_map_1.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Half map: #2
File | emd_35949_half_map_2.map | ||||||||||||
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Projections & Slices |
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Density Histograms |
-Sample components
-Entire : Human ZnT1-D47N/D255N mutant homodimer
Entire | Name: Human ZnT1-D47N/D255N mutant homodimer |
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Components |
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-Supramolecule #1: Human ZnT1-D47N/D255N mutant homodimer
Supramolecule | Name: Human ZnT1-D47N/D255N mutant homodimer / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all |
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Source (natural) | Organism: Homo sapiens (human) |
-Macromolecule #1: Proton-coupled zinc antiporter SLC30A1
Macromolecule | Name: Proton-coupled zinc antiporter SLC30A1 / type: protein_or_peptide / ID: 1 / Number of copies: 2 / Enantiomer: LEVO |
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Source (natural) | Organism: Homo sapiens (human) |
Molecular weight | Theoretical: 55.360633 KDa |
Recombinant expression | Organism: Homo sapiens (human) |
Sequence | String: MGCWGRNRGR LLCMLALTFM FMVLEVVVSR VTSSLAMLSD SFHMLSNVLA LVVALVAERF ARRTHATQKN TFGWIRAEVM GALVNAIFL TGLCFAILLE AIERFIEPHE MQQPLVVLGV GVAGLLVNVL GLCLFHHHSG FSQDSGHGHS HGGHGHGHGL P KGPRVKST ...String: MGCWGRNRGR LLCMLALTFM FMVLEVVVSR VTSSLAMLSD SFHMLSNVLA LVVALVAERF ARRTHATQKN TFGWIRAEVM GALVNAIFL TGLCFAILLE AIERFIEPHE MQQPLVVLGV GVAGLLVNVL GLCLFHHHSG FSQDSGHGHS HGGHGHGHGL P KGPRVKST RPGSSDINVA PGEQGPDQEE TNTLVANTSN SNGLKLDPAD PENPRSGDTV EVQVNGNLVR EPDHMELEED RA GQLNMRG VFLHVLGNAL GSVIVVVNAL VFYFSWKGCS EGDFCVNPCF PDPCKAFVEI INSTHASVYE AGPCWVLYLD PTL CVVMVC ILLYTTYPLL KESALILLQT VPKQIDIRNL IKELRNVEGV EEVHELHVWQ LAGSRIIATA HIKCEDPTSY MEVA KTIKD VFHNHGIHAT TIQPEFASVG SKSSVVPCEL ACRTQCALKQ CCGTLPQAPS GKDAEKTPAV SISCLELSNN LEKKP RRTK AENIPAVVIE IKNMPNKQPE SSL UniProtKB: Proton-coupled zinc antiporter SLC30A1 |
-Experimental details
-Structure determination
Method | cryo EM |
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Processing | single particle reconstruction |
Aggregation state | particle |
-Sample preparation
Buffer | pH: 7.4 |
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Grid | Model: Quantifoil / Material: GOLD / Mesh: 300 |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN |
Electron optics | Illumination mode: OTHER / Imaging mode: DIFFRACTION / Nominal defocus max: 2.3000000000000003 µm / Nominal defocus min: 1.5 µm |
Image recording | Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 50.0 e/Å2 |
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
-Image processing
Startup model | Type of model: INSILICO MODEL |
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Initial angle assignment | Type: MAXIMUM LIKELIHOOD |
Final angle assignment | Type: MAXIMUM LIKELIHOOD |
Final reconstruction | Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 550472 |