+Open data
-Basic information
Entry | Database: PDB / ID: 8hrm | ||||||
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Title | Cryo-EM structure of streptavidin | ||||||
Components | Streptavidin | ||||||
Keywords | CYTOSOLIC PROTEIN / Complex | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Streptomyces avidinii (bacteria) | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 2.56 Å | ||||||
Authors | Xu, J. / Liu, N. / Wang, H.W. | ||||||
Funding support | China, 1items
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Citation | Journal: To Be Published Title: Self-assembled monolayers guided free-standing atomic-crystal/molecule superstructure Authors: Zheng, L. / Xu, J. / Wang, W. / Gao, X. / Liu, N. / Wang, H.W. / Peng, H.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8hrm.cif.gz | 94.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8hrm.ent.gz | 73.5 KB | Display | PDB format |
PDBx/mmJSON format | 8hrm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8hrm_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 8hrm_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 8hrm_validation.xml.gz | 24.4 KB | Display | |
Data in CIF | 8hrm_validation.cif.gz | 34.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hr/8hrm ftp://data.pdbj.org/pub/pdb/validation_reports/hr/8hrm | HTTPS FTP |
-Related structure data
Related structure data | 34978MC 8hriC 8hrjC 8hrkC 8hrlC 8hrnC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 12596.641 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces avidinii (bacteria) / Production host: Escherichia coli (E. coli) / References: UniProt: P22629 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: streptavidin / Type: COMPLEX / Details: on graphene/SAMs membranes / Entity ID: #1 / Source: RECOMBINANT |
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Source (natural) | Organism: Streptomyces avidinii (bacteria) |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 7.8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 1500 nm / Nominal defocus min: 1200 nm |
Image recording | Electron dose: 50 e/Å2 / Film or detector model: GATAN K3 BIOQUANTUM (6k x 4k) |
-Processing
CTF correction | Type: NONE |
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3D reconstruction | Resolution: 2.56 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 99871 / Symmetry type: POINT |