ジャーナル: Proc Natl Acad Sci U S A / 年: 2023 タイトル: Fine structure and assembly pattern of a minimal myophage Pam3. 著者: Feng Yang / Yong-Liang Jiang / Jun-Tao Zhang / Jie Zhu / Kang Du / Rong-Cheng Yu / Zi-Lu Wei / Wen-Wen Kong / Ning Cui / Wei-Fang Li / Yuxing Chen / Qiong Li / Cong-Zhao Zhou / 要旨: The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail ...The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology.
A: Pam3 portal protein B: Pam3 portal protein C: Pam3 portal protein D: Pam3 portal protein E: Pam3 portal protein F: Pam3 portal protein G: Pam3 portal protein H: Pam3 portal protein I: Pam3 portal protein J: Pam3 portal protein K: Pam3 portal protein L: Pam3 portal protein a: Pam3 adaptor protein b: Pam3 adaptor protein c: Pam3 adaptor protein d: Pam3 adaptor protein e: Pam3 adaptor protein f: Pam3 adaptor protein g: Pam3 adaptor protein h: Pam3 adaptor protein i: Pam3 adaptor protein j: Pam3 adaptor protein k: Pam3 adaptor protein l: Pam3 adaptor protein