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Structure paper

TitleFine structure and assembly pattern of a minimal myophage Pam3.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 120, Issue 4, Page e2213727120, Year 2023
Publish dateJan 24, 2023
AuthorsFeng Yang / Yong-Liang Jiang / Jun-Tao Zhang / Jie Zhu / Kang Du / Rong-Cheng Yu / Zi-Lu Wei / Wen-Wen Kong / Ning Cui / Wei-Fang Li / Yuxing Chen / Qiong Li / Cong-Zhao Zhou /
PubMed AbstractThe myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail ...The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology.
External linksProc Natl Acad Sci U S A / PubMed:36656854 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution3.0 - 3.96 Å
Structure data

33799

7yfw

EMDB-33799, PDB-7yfw:
Cyanophage Pam3 fiber
Method: EM (single particle) / Resolution: 3.96 Å

33802

7yfz

EMDB-33802, PDB-7yfz:
Cyanophage Pam3 baseplate proteins
Method: EM (single particle) / Resolution: 3.19 Å

34678

8hdr

EMDB-34678, PDB-8hdr:
Cyanophage Pam3 neck
Method: EM (single particle) / Resolution: 3.66 Å

34679

8hds

EMDB-34679, PDB-8hds:
Cyanophage Pam3 portal-adaptor
Method: EM (single particle) / Resolution: 3.57 Å

34680

8hdt

EMDB-34680, PDB-8hdt:
Cyanophage Pam3 capsid asymmetric unit
Method: EM (single particle) / Resolution: 3.17 Å

34681

8hdw

EMDB-34681, PDB-8hdw:
Cyanophage Pam3 Sheath-tube
Method: EM (helical sym.) / Resolution: 3.0 Å

Source
  • uncultured cyanophage (environmental samples)
KeywordsVIRAL PROTEIN / fiber / VIRUS / baseplate / portal-adaptor / capsid asymmetric unit / Sheath-tube

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