ジャーナル: Proc Natl Acad Sci U S A / 年: 2023 タイトル: Fine structure and assembly pattern of a minimal myophage Pam3. 著者: Feng Yang / Yong-Liang Jiang / Jun-Tao Zhang / Jie Zhu / Kang Du / Rong-Cheng Yu / Zi-Lu Wei / Wen-Wen Kong / Ning Cui / Wei-Fang Li / Yuxing Chen / Qiong Li / Cong-Zhao Zhou / 要旨: The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail ...The myophage possesses a contractile tail that penetrates its host cell envelope. Except for investigations on the bacteriophage T4 with a rather complicated structure, the assembly pattern and tail contraction mechanism of myophage remain largely unknown. Here, we present the fine structure of a freshwater cyanophage Pam3, which has an icosahedral capsid of ~680 Å in diameter, connected via a three-section neck to an 840-Å-long contractile tail, ending with a three-module baseplate composed of only six protein components. This simplified baseplate consists of a central hub-spike surrounded by six wedge heterotriplexes, to which twelve tail fibers are covalently attached via disulfide bonds in alternating upward and downward configurations. In vitro reduction assays revealed a putative redox-dependent mechanism of baseplate assembly and tail sheath contraction. These findings establish a minimal myophage that might become a user-friendly chassis phage in synthetic biology.
1: pam3 tube protein 2: pam3 tube protein A: Pam3 connector protein B: Pam3 connector protein C: Pam3 connector protein D: Pam3 connector protein E: Pam3 connector protein F: Pam3 connector protein G: Pam3 terminator protein H: Pam3 terminator protein I: Pam3 terminator protein J: Pam3 terminator protein K: Pam3 terminator protein L: Pam3 terminator protein M: Pam3 sheath protein N: Pam3 sheath protein O: Pam3 sheath protein P: Pam3 sheath protein Q: Pam3 sheath protein R: Pam3 sheath protein S: Pam3 sheath protein T: Pam3 sheath protein U: Pam3 sheath protein V: Pam3 sheath protein W: Pam3 sheath protein X: Pam3 sheath protein Y: pam3 tube protein Z: pam3 tube protein a: Pam3 adaptor protein b: Pam3 adaptor protein c: Pam3 adaptor protein d: Pam3 adaptor protein e: Pam3 adaptor protein f: Pam3 adaptor protein g: Pam3 adaptor protein h: Pam3 adaptor protein i: Pam3 adaptor protein j: Pam3 adaptor protein k: Pam3 adaptor protein l: Pam3 adaptor protein m: pam3 tube protein n: pam3 tube protein o: pam3 tube protein p: pam3 tube protein q: pam3 tube protein r: pam3 tube protein s: pam3 tube protein t: pam3 tube protein u: pam3 tube protein v: pam3 tube protein w: pam3 tube protein x: pam3 tube protein y: pam3 tube protein z: pam3 tube protein