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Yorodumi- PDB-8g6v: Hepatitis B virus capsid bound to importin alpha1/beta heterodimer -
+Open data
-Basic information
Entry | Database: PDB / ID: 8g6v | ||||||
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Title | Hepatitis B virus capsid bound to importin alpha1/beta heterodimer | ||||||
Components | Core protein Cp183 | ||||||
Keywords | VIRUS LIKE PARTICLE / complex formed by viral capsid and importin | ||||||
Function / homology | Hepatitis B virus, capsid N-terminal / Hepatitis core protein, putative zinc finger / Hepatitis core antigen / Viral capsid core domain supefamily, Hepatitis B virus / Hepatitis core antigen / virus-mediated perturbation of host defense response / structural molecule activity / extracellular region / External core antigen Function and homology information | ||||||
Biological species | Hepatitis B virus | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 3.4 Å | ||||||
Authors | Yang, R. / Cingolani, G. | ||||||
Funding support | United States, 1items
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Citation | Journal: To Be Published Title: Structure of Hepatitis B virus capsid bound to importin alpha1/beta heterodimer Authors: Yang, R. / Cingolani, G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 8g6v.cif.gz | 337.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb8g6v.ent.gz | 278.8 KB | Display | PDB format |
PDBx/mmJSON format | 8g6v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 8g6v_validation.pdf.gz | 1.4 MB | Display | wwPDB validaton report |
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Full document | 8g6v_full_validation.pdf.gz | 1.4 MB | Display | |
Data in XML | 8g6v_validation.xml.gz | 62.2 KB | Display | |
Data in CIF | 8g6v_validation.cif.gz | 92.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/g6/8g6v ftp://data.pdbj.org/pub/pdb/validation_reports/g6/8g6v | HTTPS FTP |
-Related structure data
Related structure data | 29785MC 7umiC 8g5vC 8g8yC M: map data used to model this data C: citing same article (ref.) |
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Similar structure data | Similarity search - Function & homologyF&H Search |
-Links
-Assembly
Deposited unit |
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1 |
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-Components
#1: Protein | Mass: 21146.217 Da / Num. of mol.: 12 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Hepatitis B virus / Production host: Escherichia coli (E. coli) / References: UniProt: W6CP35 |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
-Sample preparation
Component | Name: Complex of Cp183 when bound to importin alpha1/beta at the quasi-sixfold pore Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: Hepatitis B virus |
Source (recombinant) | Organism: Escherichia coli (E. coli) |
Buffer solution | pH: 8 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
-Electron microscopy imaging
Experimental equipment | Model: Titan Krios / Image courtesy: FEI Company |
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Microscopy | Model: FEI TITAN KRIOS |
Electron gun | Electron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM |
Electron lens | Mode: BRIGHT FIELD / Nominal defocus max: 2000 nm / Nominal defocus min: 800 nm |
Image recording | Electron dose: 1.25 e/Å2 / Film or detector model: GATAN K3 (6k x 4k) |
-Processing
Software | Name: PHENIX / Version: 1.20.1_4487: / Classification: refinement | ||||||||||||||||||||||||
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CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||
3D reconstruction | Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 16181 / Symmetry type: POINT | ||||||||||||||||||||||||
Refine LS restraints |
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