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-基本情報
登録情報 | データベース: PDB / ID: 8a2y | |||||||||||||||
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タイトル | Cryo-EM structure of F-actin in the Ca2+-ADP-Pi nucleotide state. | |||||||||||||||
要素 | Actin, alpha skeletal muscle | |||||||||||||||
キーワード | STRUCTURAL PROTEIN / actin / cytoskeleton / filament / nucleotide state | |||||||||||||||
機能・相同性 | 機能・相同性情報 cytoskeletal motor activator activity / tropomyosin binding / myosin heavy chain binding / mesenchyme migration / troponin I binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / actin filament bundle assembly / striated muscle thin filament ...cytoskeletal motor activator activity / tropomyosin binding / myosin heavy chain binding / mesenchyme migration / troponin I binding / filamentous actin / actin filament bundle / skeletal muscle thin filament assembly / actin filament bundle assembly / striated muscle thin filament / skeletal muscle myofibril / actin monomer binding / skeletal muscle fiber development / stress fiber / titin binding / actin filament polymerization / filopodium / actin filament / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / calcium-dependent protein binding / lamellipodium / cell body / hydrolase activity / protein domain specific binding / calcium ion binding / positive regulation of gene expression / magnesium ion binding / ATP binding / identical protein binding / cytoplasm 類似検索 - 分子機能 | |||||||||||||||
生物種 | Oryctolagus cuniculus (ウサギ) | |||||||||||||||
手法 | 電子顕微鏡法 / 単粒子再構成法 / クライオ電子顕微鏡法 / 解像度: 2.15 Å | |||||||||||||||
データ登録者 | Oosterheert, W. / Klink, B.U. / Belyy, A. / Pospich, S. / Raunser, S. | |||||||||||||||
資金援助 | European Union, ドイツ, 4件
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引用 | ジャーナル: Nature / 年: 2022 タイトル: Structural basis of actin filament assembly and aging. 著者: Wout Oosterheert / Björn U Klink / Alexander Belyy / Sabrina Pospich / Stefan Raunser / 要旨: The dynamic turnover of actin filaments (F-actin) controls cellular motility in eukaryotes and is coupled to changes in the F-actin nucleotide state. It remains unclear how F-actin hydrolyses ATP and ...The dynamic turnover of actin filaments (F-actin) controls cellular motility in eukaryotes and is coupled to changes in the F-actin nucleotide state. It remains unclear how F-actin hydrolyses ATP and subsequently undergoes subtle conformational rearrangements that ultimately lead to filament depolymerization by actin-binding proteins. Here we present cryo-electron microscopy structures of F-actin in all nucleotide states, polymerized in the presence of Mg or Ca at approximately 2.2 Å resolution. The structures show that actin polymerization induces the relocation of water molecules in the nucleotide-binding pocket, activating one of them for the nucleophilic attack of ATP. Unexpectedly, the back door for the subsequent release of inorganic phosphate (P) is closed in all structures, indicating that P release occurs transiently. The small changes in the nucleotide-binding pocket after ATP hydrolysis and P release are sensed by a key amino acid, amplified and transmitted to the filament periphery. Furthermore, differences in the positions of water molecules in the nucleotide-binding pocket explain why Ca-actin shows slower polymerization rates than Mg-actin. Our work elucidates the solvent-driven rearrangements that govern actin filament assembly and aging and lays the foundation for the rational design of drugs and small molecules for imaging and therapeutic applications. | |||||||||||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 8a2y.cif.gz | 396.5 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb8a2y.ent.gz | 274.5 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 8a2y.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 8a2y_validation.pdf.gz | 1.6 MB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 8a2y_full_validation.pdf.gz | 1.6 MB | 表示 | |
XML形式データ | 8a2y_validation.xml.gz | 64.3 KB | 表示 | |
CIF形式データ | 8a2y_validation.cif.gz | 97 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/a2/8a2y ftp://data.pdbj.org/pub/pdb/validation_reports/a2/8a2y | HTTPS FTP |
-関連構造データ
関連構造データ | 15108MC 8a2rC 8a2sC 8a2tC 8a2uC 8a2zC C: 同じ文献を引用 (文献) M: このデータのモデリングに利用したマップデータ |
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類似構造データ | 類似検索 - 機能・相同性F&H 検索 |
-リンク
-集合体
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非結晶学的対称性 (NCS) | NCSドメイン:
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