National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
R01 AI136680
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
AI27690
米国
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)
U54AI150472
米国
引用
ジャーナル: Sci Adv / 年: 2022 タイトル: Cryo-EM structure of the HIV-1 Pol polyprotein provides insights into virion maturation. 著者: Jerry Joe E K Harrison / Dario Oliveira Passos / Jessica F Bruhn / Joseph D Bauman / Lynda Tuberty / Jeffrey J DeStefano / Francesc Xavier Ruiz / Dmitry Lyumkis / Eddy Arnold / 要旨: Key proteins of retroviruses and other RNA viruses are translated and subsequently processed from polyprotein precursors by the viral protease (PR). Processing of the HIV Gag-Pol polyprotein yields ...Key proteins of retroviruses and other RNA viruses are translated and subsequently processed from polyprotein precursors by the viral protease (PR). Processing of the HIV Gag-Pol polyprotein yields the HIV structural proteins and enzymes. Structures of the mature enzymes PR, reverse transcriptase (RT), and integrase (IN) aided understanding of catalysis and design of antiretrovirals, but knowledge of the Pol precursor architecture and function before PR cleavage is limited. We developed a system to produce stable HIV-1 Pol and determined its cryo-electron microscopy structure. RT in Pol has a similar arrangement to the mature RT heterodimer, and its dimerization may draw together two PR monomers to activate proteolytic processing. HIV-1 thus may leverage the dimerization interfaces in Pol to regulate assembly and maturation of polyprotein precursors.
分子量: 119289.867 Da / 分子数: 2 / 断片: PR-RT portion, residues 479-1447 / 変異: D131A, L765D, F766D / 由来タイプ: 組換発現 由来: (組換発現) Human immunodeficiency virus type 1 group M subtype B (isolate BH10) (ヒト免疫不全ウイルス) 株: isolate BH10 / 遺伝子: gag-pol / 発現宿主: Escherichia coli BL21(DE3) (大腸菌) / 株 (発現宿主): BL21(DE3) / 参照: UniProt: P03366
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実験情報
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実験
実験
手法: 電子顕微鏡法
EM実験
試料の集合状態: PARTICLE / 3次元再構成法: 単粒子再構成法
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試料調製
構成要素
名称: PR-RT portion of HIV-1 Pol / タイプ: COMPLEX 詳細: 3D reconstruction of the HIV-1 Pol polyprotein comprising the PR-RT portion Entity ID: all / 由来: RECOMBINANT
分子量
値: 0.13 MDa / 実験値: NO
由来(天然)
生物種: Human immunodeficiency virus type 1 BH10 (ヒト免疫不全ウイルス)